scholarly journals Multiple insecticide resistance mechanisms in Anopheles gambiae s.l. populations from Cameroon, Central Africa

2013 ◽  
Vol 6 (1) ◽  
Author(s):  
Philippe Nwane ◽  
Josiane Etang ◽  
Mouhamadou Chouaїbou ◽  
Jean Claude Toto ◽  
Alphonsine Koffi ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Central Africa ◽  
Resistance Mechanisms ◽  
Anopheles Gambiae S.L

scholarly journals Phenotypic Insecticide Resistance in Anopheles gambiae (Diptera: Culicidae): Specific Characterization of Underlying Resistance Mechanisms Still Matters

2020 ◽  
Author(s):  
Adandé A Medjigbodo ◽  
Luc S Djogbenou ◽  
Aubin A Koumba ◽  
Laurette Djossou ◽  
Athanase Badolo ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Resistance Mechanisms ◽  
Malaria Vectors ◽  
Effective Control ◽  
Anopheles Coluzzii ◽  
Anopheles Gambiae S.S ◽  
Diagnostic Dose ◽  
Pirimiphos Methyl ◽  
Old Adult

Abstract An effective control of malaria vectors requires an extensive knowledge of mechanisms underlying the resistance-phenotypes developed by these vectors against insecticides. We investigated Anopheles gambiae mosquitoes from Benin and Togo for their intensity of insecticide resistance and we discussed the involvement of genotyped mechanisms in the resistance-phenotypes observed. Three- to five-day-old adult mosquitoes emerged from field and laboratory An. gambiae larvae were assayed using WHO tube intensity tests against various doses of deltamethrin: 1× (0.05%); 2× (0.1%); 5× (0.25%); 7.5× (0.375%) and those of pirimiphos-methyl: 0.5× (0.125%); 1× (0.25%). Members of An. gambiae complex were screened in field populations using polymerase chain reaction (PCR) assays. The presence of kdrR(1014F/1014S) and ace-1R(119S) mutations was also investigated using TaqMan and PCR-RFLP techniques, respectively. Anopheles gambiae from field were very resistant to deltamethrin, whereas KisKdr and AcerKdrKis strains displayed 100% mortality rates at 2× the diagnostic dose. In contrast, the field mosquitoes displayed a low resistance-intensity against 1× the diagnostic dose of pirimiphos-methyl, whereas AcerKis and AcerKdrKis strains showed susceptibility at 0.5× the diagnostic dose. Anopheles gambiae s.s., Anopheles coluzzii, and Anopheles arabiensis were identified. Allelic frequencies of kdrR (1014F) and ace-1R (119S) mutations in the field populations varied from 0.65 to 1 and 0 to 0.84, respectively. The field An. gambiae displayed high-resistance levels against deltamethrin and pirimiphos-methyl when compared with those of the laboratory An. gambiae-resistant strains. These results exhibit the complexity of underlying insecticide resistance mechanisms in these field malaria vectors.

scholarly journals High insecticide resistance intensity of Anopheles gambiae (s.l.) and low efficacy of pyrethroid LLINs in Accra, Ghana

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Rebecca Pwalia ◽  
Joannitta Joannides ◽  
Alidu Iddrisu ◽  
Charlotte Addae ◽  
Dominic Acquah-Baidoo ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Anopheles Gambiae S.L

scholarly journals Multiple Insecticide Resistance in Anopheles gambiae s.l. Populations from Burkina Faso, West Africa

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48412 ◽  
Author(s):  
Moussa Namountougou ◽  
Frédéric Simard ◽  
Thierry Baldet ◽  
Abdoulaye Diabaté ◽  
Jean Bosco Ouédraogo ◽  
...  
Keyword(s):  
West Africa ◽  
Insecticide Resistance ◽  
Burkina Faso ◽  
Anopheles Gambiae ◽  
Anopheles Gambiae S.L

The distribution of insecticide resistance in Anopheles gambiae s.l. populations from Cameroon: an update

2009 ◽  
Vol 103 (11) ◽  
pp. 1127-1138 ◽  
Author(s):  
Hamadou N.M. Ndjemaï ◽  
Salomon Patchoké ◽  
Jean Atangana ◽  
Josiane Etang ◽  
Fréderic Simard ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Anopheles Gambiae S.L

scholarly journals Widespread Report of Multiple Insecticide Resistance in Anopheles gambiae s.l. Mos-quitoes in Eight Communities in Southern Gombe, North-Eastern Nigeria

2019 ◽  
Author(s):  
Adedayo Olatunbosun-Oduola ◽  
Ezra Abba ◽  
Olukayode Adelaja ◽  
Adeolu Taiwo-Ande ◽  
Kennedy Poloma-Yoriyo ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Management Strategies ◽  
Resistance Management ◽  
Current Control ◽  
Control Programs ◽  
Anopheles Mosquitoes ◽  
Exposed Population ◽  
Pirimiphos Methyl ◽  
Anopheles Gambiae S.L

Background: Timely entomological and insecticide resistance monitoring is a key to generating relevant data for vector management. We investigated the insecticide susceptibility status of Anopheles gambiae s.l. in eight rural farming communities in Southern Gombe, Nigeria. Methods: Overall, 3–5 days-old adult female Anopheles mosquitoes reared from field-collected immature stages between September and November, 2014 were exposed to the diagnostic doses of pyrethroids, organophosphate and carbamate insecticides using the Center for Disease Control Bottle bioassay. The observatory knockdown time from exposure to each insecticide was recorded up to two hours. The dead mosquitoes were then identified morphological­ly and by molecular assays. Results: Mortality results showed resistance in An. gambiae s.l. populations to bendiocarb (2.3–100%), deltamethrin (39–70%), pirimiphos-methyl (65–95%), dichloro-diphenyl-trichloroethane (0–38.1%), permethrin (0–46.3%) and lambda-cyhalothrin (42.5–86.4%). The few cases of full susceptibility were observed from lamdacyhalothrin exposed population of An. gambiae s.l. in Banbam and Pantami respectively. An. gambiae 177 (45%) was significantly higher (P< 0.05) than An. arabiensis 64 (16.3%), An. coluzzii 34 (8.7%) and An. gambiae/An. coluzzii hybrid 78 (19.8%). Conclusion: A strong evidence of widespread resistance in the major malaria vector species in Southern Gombe to all common classes of insecticides is a justification for the State Malaria Elimination Programme to consciously con­sider incorporating insecticide resistance management strategies into control programs in order to sustain the future of current control interventions

scholarly journals Functional genetic validation of key genes conferring insecticide resistance in the major African malaria vector, Anopheles gambiae

2019 ◽  
Author(s):  
Adriana Adolfi ◽  
Beth Poulton ◽  
Amalia Anthousi ◽  
Stephanie Macilwee ◽  
Hilary Ranson ◽  
...  
Keyword(s):  
Public Health ◽  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Pyrethroid Resistance ◽  
Control Strategies ◽  
Resistance Mechanisms ◽  
Effective Control ◽  
Cross Resistance ◽  
Toxic Metabolite ◽  
Control Programs

ABSTRACTResistance in Anopheles gambiae to members of all four major classes (pyrethroids, carbamates, organochlorines and organophosphates) of public health insecticides limits effective control of malaria transmission in Africa. Increased expression of detoxifying enzymes has been associated with resistance, but direct functional validation in An. gambiae has been lacking. Here we perform transgenic analysis using the GAL4/UAS system to examine insecticide resistance phenotypes conferred by increased expression of the three genes - Cyp6m2, Cyp6p3 and Gste2 - most often found upregulated in resistant An. gambiae. We report the first evidence in An. gambiae that organophosphate and organochlorine resistance is conferred by overexpression of GSTE2 in a broad tissue profile. Pyrethroid and carbamate resistance is bestowed by similar Cyp6p3 overexpression, and Cyp6m2 confers only pyrethroid resistance when overexpressed in the same tissues. Conversely, such Cyp6m2 overexpression increases susceptibility to the organophosphate malathion, presumably due to conversion to a more toxic metabolite. No resistant phenotypes are conferred when either Cyp6 gene overexpression is restricted to the midgut or oenocytes, answering long standing questions related to the importance of these tissues in resistance to contact insecticides. Validation of genes conferring resistance provides markers to guide control strategies, and the observed negative cross-resistance due to Cyp6m2 gives credence to proposed dual insecticide strategies to overcome pyrethroid resistance. These trasnsgenic An. gambiae resistant lines are being used to test potential liabilities in new active compounds early in development.SIGNIFICANCE STATEMENTInsecticide resistance in Anopheles gambiae mosquitoes can derail malaria control programs, and to overcome it we need to discover the underlying molecular basis. Here, for the first time, we characterise three genes most often associated with insecticide resistance directly by their overproduction in genetically modified An. gambiae. We show that overexpression of each gene confers resistance to representatives of at least one insecticide class and, taken together, the three genes provide cross-resistance to all four major insecticide classes currently used in public health. These data validate the candidate genes as markers to monitor the spread of resistance in mosquito populations. The modified mosquitoes produced are also valuable tools to pre-screen new insecticides for potential liabilities to existing resistance mechanisms.

scholarly journals The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Emma Collins ◽  
Natasha M. Vaselli ◽  
Moussa Sylla ◽  
Abdoul H. Beavogui ◽  
James Orsborne ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Malaria Prevalence ◽  
Anopheles Gambiae S.L ◽  
The Relationship

scholarly journals Insecticide resistance mechanisms associated with different environments in the malaria vector Anopheles gambiae: a case study in Tanzania

2014 ◽  
Vol 13 (1) ◽  
pp. 28 ◽  
Author(s):  
Theresia E Nkya ◽  
Idir Akhouayri ◽  
Rodolphe Poupardin ◽  
Bernard Batengana ◽  
Franklin Mosha ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Resistance Mechanisms
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