The Proliferation of Germ Cells in the Testes of Bull Calves and Young Bulls

The aim of the present study was to evaluate the effects of gonadotropin administration at initiation of inhibin passive immunisation in Jersey bull calves (age 27 � 5 days) on testicular morphology and development. Primary treatments consisted of control (keyhole limpet haemocyanin, KLH; n = 9) or immunisation against inhibin (INH; n = 9). Subsets of calves were randomly assigned within primary treatments (TRT) to receive saline ( n = 3 per TRT), follicle-stimulating hormone (FSH; n = 3 per TRT) or gonadotrophin-releasing hormone (GnRH, n = 3 per TRT). The right testis was removed (age 118 � 5 days) to determine volumes of testicular components and cell numbers per testis using stereology. Data were analysed using the MIXED procedure of the SAS program. Antibody titres against inhibin were increased in INH bulls compared with KLH bulls (P < 0.05). In addition, a significant immunisation � hormone treatment interaction was noted for the number of germ cells. Administration of FSH at the time of initial immunisation against inhibin significantly increased the number of germ cells (92.2 � 9 � 106 cells) compared with INH+saline bulls (54.9 � 10 � 106 cells), with INH+GnRH bulls having an intermediate number of cells (64.5 � 9 � 106 cells; P < 0.05). These results suggest that gonadotropin administration at the time of inhibin immunisation increases the number of germ cells in the testis.

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Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation

Cell and Tissue Research ◽

10.1007/s00441-007-0445-z ◽

2007 ◽

Vol 330 (2) ◽

pp. 321-329 ◽

Cited By ~ 42

Author(s):

Muren Herrid ◽

Rhonda J. Davey ◽

Jonathan R. Hill

Keyword(s):

Germ Cell ◽

Cell Transplantation ◽

Germ Cells ◽

Germ Cell Transplantation ◽

Bull Calves

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Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008256x ◽

1978 ◽

Vol 36 (2) ◽

pp. 340-341

Author(s):

Rita Meyer ◽

Zoltan Posalaky ◽

Dennis Mcginley

Keyword(s):

Organ Culture ◽

Tight Junctions ◽

Sertoli Cell ◽

Germ Cells ◽

Sertoli Cells ◽

Barrier Function ◽

Cell Junction ◽

Intramembranous Particles ◽

Junctional Complexes ◽

Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

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Comparative Morphology of Intercellular Bridges Between Developing Germ Cells of the Ovary

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068631 ◽

1970 ◽

Vol 28 ◽

pp. 328-329

Author(s):

J. R. Ruby ◽

R. F. Dyer ◽

R. G. Skalko ◽

R. F. Gasser ◽

E. P. Volpe

Keyword(s):

Germ Cells ◽

Rana Pipiens ◽

Comparative Morphology ◽

Dense Material ◽

Individual Species ◽

Microscope Examination ◽

Electron Dense Material ◽

Intercellular Bridges ◽

Cytoplasmic Side ◽

Intercellular Connections

An electron microscope examination of fetal ovaries has revealed that developing germ cells are connected by intercellular bridges. In this investigation several species have been studied including human, mouse, chicken, and tadpole (Rana pipiens). These studies demonstrate that intercellular connections are similar in morphology regardless of the species.Basically, all bridges are characterized by a band of electron-dense material on the cytoplasmic side of the tri-laminar membrane surrounding the connection (Fig.l). This membrane is continuous with the plasma membrane of the conjoined cells. The dense material, however, never extends beyond the limits of the bridge. Variations in the configuration of intercellular connections were noted in all ovaries studied. However, the bridges in each individual species usually exhibits one structural characteristic seldom found in the others. For example, bridges in the human ovary very often have large blebs projecting from the lateral borders whereas the sides of the connections in the mouse gonad merely demonstrate a slight convexity.

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Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012374x ◽

1992 ◽

Vol 50 (1) ◽

pp. 668-669

Author(s):

Amreek Singh ◽

Warren G. Foster ◽

Anna Dykeman ◽

David C. Villeneuve

Keyword(s):

Germ Cells ◽

Primordial Germ Cells ◽

Surface Epithelium ◽

Morphological Parameters ◽

Comprehensive Investigation ◽

Ovary Surface Epithelium ◽

Rat Ovary ◽

High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

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Electron microscope study of the effects of radiation on insect fertility

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157905 ◽

1990 ◽

Vol 48 (3) ◽

pp. 72-73

Author(s):

Judy Ju-Hu Chiang ◽

Robert Kuo-Cheng Chen

Keyword(s):

Electron Microscopy ◽

Germ Cell ◽

Germ Cells ◽

Electron Microscope Study ◽

Radiation Treatment ◽

Light And Electron Microscopy ◽

Stem Borer ◽

Metabolic Energy ◽

Rice Stem Borer ◽

Effects Of Radiation

Germ cells from the rice stem borer Chilo suppresalis, were examined by light and electron microscopy. Damages to organelles within the germ cells were observed. The mitochondria, which provide the cell with metabolic energy, were seen to disintegrate within the germ cell. Lysosomes within the germ cell were also seen to disintegrate. The subsequent release of hydrolytic enzymesmay be responsible for the destruction of organelles within the germ cell. Insect spermatozoa were seen to lose the ability to move because of radiation treatment. Damage to the centrioles, one of which is in contact with the tail, may be involved in causing sperm immobility.

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1376: Molecular Diagnostic Detection of Haploid Germ Cells in Testicular Biopsy Specimens by Online RT-PCR

The Journal of Urology ◽

10.1016/s0022-5347(18)38601-4 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 362-363

Author(s):

Mark G. Schrader ◽

Markus Muller ◽

Wolfgang Schulze ◽

Steffen Weikert ◽

Kurt Miller

Keyword(s):

Germ Cells ◽

Testicular Biopsy ◽

Molecular Diagnostic ◽

Rt Pcr ◽

Biopsy Specimens ◽

Diagnostic Detection

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INFLUENCE OF HIGH-PROTEIN FEED ADDITIVE ON THE PRODUCTIVITY OF THE MEAT BULL-CALVES

Adaptive Fodder Production ◽

10.33814/afp-2222-5366-2019-3-6-11 ◽

2019 ◽

Vol 2019 (3) ◽

pp. 6-11

Author(s):

Батырхан Абилов ◽

Batyrkhan Abilov ◽

Лариса Пашкова ◽

Larisa Pashkova

Keyword(s):

Hematological Parameters ◽

Physiological State ◽

Average Daily Gain ◽

Live Weight ◽

Feed Additive ◽

North Caucasus ◽

Agricultural Enterprise ◽

Bull Calves ◽

Economic Indices ◽

High Level

The article presents the data obtained as a result of carrying by employees of the Research Institute of sheep and goat breeding, a branch of the FSBSI "North Caucasus FARC", the scientific and economic experience to study the efficiency of a new fodder additive "Organic" on the productive and physiological indices of bull-calves during the completion of growing in the conditions of the farm "Pata" agricultural enterprise in the Karachay-Cherkessia Republic. The statement of the experience and all researches were carried out according to generally accepted methods. In the course of the experiment, the following indices were studied: the chemical compound of forages, the dynamics of a live weight gain in the experimental stock, hematological parameters, control slaughter data, economic indices. Completed researches have shown positive result from the use of the fodder additive "Organic" in bull-calves diets: an increase in the average daily gain of live weight by 25%, a slaughter yield by 1% and a high level of profitability – 27%. Thus, the additional introduction of "Organic" in a diet of young horned cattle in the 2nd experimental group promoted more intensive metabolism, stabilization of a physiological state, increase in productivity and, as a consequence, profitability of beef production. Researches in this direction reveal the latent fodder reserves promoting increase in the productivity of agricultural animals.

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