scholarly journals Nucleic acid amplification-based pathogen detection in the blood of severe sepsis patients

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P43 ◽  
Author(s):  
Frank Bloos ◽  
Svea Sachse ◽  
Karl-Herrmann Schmidt ◽  
Mark Lehmann ◽  
Roland Schmitz ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Nucleic Acid ◽  
Pathogen Detection ◽  
Nucleic Acid Amplification

Limited-Resource Preparable Chitosan Magnetic Particles for Extracting Amplification-Ready Nucleic Acid from Complex Biofluid

The Analyst ◽  
2021 ◽  
Author(s):  
Sayantan Tripathy ◽  
Ashish Chalana ◽  
Arunansu Talukdar ◽  
P.V. Rajesh ◽  
Abhijit Saha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Magnetic Particles ◽  
Genetic Diseases ◽  
Limited Resource ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification

Extraction and concentration of pure nucleic acid from complex biofluids is the prerequisite for nucleic acid amplification test (NAAT) applications in pathogen detection, biowarfare prevention, and genetic diseases. However, conventional...

scholarly journals Nanoparticle labels for pathogen detection through nucleic acid amplification tests

2014 ◽  
Vol 19 (2) ◽  
pp. 299-305 ◽  
Author(s):  
Philip Drake ◽  
Yi-Chang Chen ◽  
Ingo Lehmann ◽  
Pei-Shin Jiang
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests

scholarly journals Nucleic Acid Amplification Strategies for DNA Microarray-Based Pathogen Detection

2004 ◽  
Vol 70 (5) ◽  
pp. 3047-3054 ◽  
Author(s):  
Gary J. Vora ◽  
Carolyn E. Meador ◽  
David A. Stenger ◽  
Joanne D. Andreadis
Keyword(s):  
Nucleic Acid ◽  
Multiplex Pcr ◽  
Dna Microarray ◽  
Pathogen Detection ◽  
Pcr Amplification ◽  
Environmental Water ◽  
Nucleic Acid Amplification ◽  
Klenow Fragment ◽  
Front End ◽  
Random Amplification

ABSTRACT DNA microarray-based screening and diagnostic technologies have long promised comprehensive testing capabilities. However, the potential of these powerful tools has been limited by front-end target-specific nucleic acid amplification. Despite the sensitivity and specificity associated with PCR amplification, the inherent bias and limited throughput of this approach constrain the principal benefits of downstream microarray-based applications, especially for pathogen detection. To begin addressing alternative approaches, we investigated four front-end amplification strategies: random primed, isothermal Klenow fragment-based, φ29 DNA polymerase-based, and multiplex PCR. The utility of each amplification strategy was assessed by hybridizing amplicons to microarrays consisting of 70-mer oligonucleotide probes specific for enterohemorrhagic Escherichia coli O157:H7 and by quantitating their sensitivities for the detection of O157:H7 in laboratory and environmental samples. Although nearly identical levels of hybridization specificity were achieved for each method, multiplex PCR was at least 3 orders of magnitude more sensitive than any individual random amplification approach. However, the use of Klenow-plus-Klenow and φ29 polymerase-plus-Klenow tandem random amplification strategies provided better sensitivities than multiplex PCR. In addition, amplification biases among the five genetic loci tested were 2- to 20-fold for the random approaches, in contrast to >4 orders of magnitude for multiplex PCR. The same random amplification strategies were also able to detect all five diagnostic targets in a spiked environmental water sample that contained a 63-fold excess of contaminating DNA. The results presented here underscore the feasibility of using random amplification approaches and begin to systematically address the versatility of these approaches for unbiased pathogen detection from environmental sources.

scholarly journals Advanced CRISPR-Cas Effector Enzyme-Based Diagnostics for Infectious Diseases, Including COVID-19

Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1356
Author(s):  
Sangha Kwon ◽  
Ha Youn Shin
Keyword(s):  
Nucleic Acid ◽  
Infectious Diseases ◽  
Pathogen Detection ◽  
Diagnostic Techniques ◽  
Diagnostic Methods ◽  
Nucleic Acid Amplification ◽  
Detection Methods ◽  
Molecular Diagnostic ◽  
Nucleic Acid Detection ◽  
Time Accuracy

Rapid and precise diagnostic tests can prevent the spread of diseases, including worldwide pandemics. Current commonly used diagnostic methods include nucleic-acid-amplification-based detection methods and immunoassays. These techniques, however, have several drawbacks in diagnosis time, accuracy, and cost. Nucleic acid amplification methods are sensitive but time-consuming, whereas immunoassays are more rapid but relatively insensitive. Recently developed CRISPR-based nucleic acid detection methods have been found to compensate for these limitations. In particular, the unique collateral enzymatic activities of Cas12 and Cas13 have dramatically reduced the diagnosis times and costs, while improving diagnostic accuracy and sensitivity. This review provides a comprehensive description of the distinct enzymatic features of Cas12 and Cas13 and their applications in the development of molecular diagnostic platforms for pathogen detection. Moreover, it describes the current utilization of CRISPR-Cas-based diagnostic techniques to identify SARS-CoV-2 infection, as well as recent progress in the development of CRISPR-Cas-based detection strategies for various infectious diseases. These findings provide insights into designing effective molecular diagnostic platforms for potential pandemics.

Nucleic acid amplification free biosensors for pathogen detection

2020 ◽  
Vol 153 ◽  
pp. 112049 ◽  
Author(s):  
Yanju Chen ◽  
Cheng Qian ◽  
Chengzhi Liu ◽  
Hong Shen ◽  
Zhijian Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Nucleic Acid Amplification
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