Identification of Blast Resistance QTLs Based on Two Advanced Backcross Populations in Rice

Abstract Background: Rice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed. Results: In this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi were as the donor parent to establish two BC 3 F 1 and derived BC 3 F 2 backcross populations in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% during six observation times. And, among Jin23B/QingGuAi population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% during six observation times. Conclusions: Sixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi.

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Identification of blast resistance QTLs based on two ad vanced backcross populations in rice

10.21203/rs.2.24273/v2 ◽

2020 ◽

Author(s):

Haichao Jiang ◽

Yutao Feng ◽

Lei Qiu ◽

Guanjun Gao ◽

Qinglu Zhang ◽

...

Keyword(s):

Phenotypic Variation ◽

Rice Blast ◽

Blast Resistance ◽

Natural Infection ◽

Rice Variety ◽

Resistance Qtls ◽

Backcross Population ◽

Advanced Backcross ◽

Minor Qtls ◽

Observation Times

Abstract Background: Rice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed.Results: In this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi were as the donor parent to establish two BC3F1 and derived BC3F2 backcross population in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% during six observation times. And, among Jin23B/QingGuAi population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% during six observation times.Conclusions: Sixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi.

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Identification of blast resistance QTLs based on two ad vanced backcross populations in rice

10.21203/rs.2.24273/v3 ◽

2020 ◽

Author(s):

Haichao Jiang ◽

Yutao Feng ◽

Lei Qiu ◽

Guanjun Gao ◽

Qinglu Zhang ◽

...

Keyword(s):

Phenotypic Variation ◽

Rice Blast ◽

Blast Resistance ◽

Natural Infection ◽

Rice Variety ◽

Resistance Qtls ◽

Backcross Population ◽

Advanced Backcross ◽

Minor Qtls ◽

Advanced Backcross Population

Abstract Background: Rice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed.Results: In this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi3 were as the donor parent to establish two BC3F1 and derived BC3F2 backcross population in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% in two years. And, among Jin23B/QingGuAi3 population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% in two years.Conclusions: Sixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi3.

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Mapping and evaluating quantitative trait loci for blast resistance under natural infection conditions using an advanced backcross population in rice

Euphytica ◽

10.1007/s10681-014-1347-2 ◽

2015 ◽

Vol 204 (1) ◽

pp. 121-133 ◽

Cited By ~ 2

Author(s):

Haichao Jiang ◽

Bin Yan ◽

Taipin Duan ◽

Yibo Li ◽

Guanjun Gao ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Blast Resistance ◽

Natural Infection ◽

Backcross Population ◽

Advanced Backcross ◽

Advanced Backcross Population ◽

Trait Loci

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Keragaan Sifat Tahan Penyakit Blas dan Agronomi Populasi Silang Balik dan Haploid Ganda Turunan IR64 dan Oryza rufipogon

Buletin Plasma Nutfah ◽

10.21082/blpn.v16n2.2010.p90-95 ◽

2016 ◽

Vol 16 (2) ◽

pp. 90

Author(s):

Dwinita W. Utami ◽

A. Dinar Ambarwati ◽

Aniversari Apriana ◽

Atmitri Sisharmini ◽

Ida Hanarida ◽

...

Keyword(s):

Double Haploid ◽

Blast Resistance ◽

Oryza Rufipogon ◽

Screening Tests ◽

Recurrent Parent ◽

Wild Rice Species ◽

Backcross Populations ◽

Haploid Population ◽

The One ◽

Resistance Performance

Blast Resistance Performance of Promising Lines Derived from Backcross and Double Haploid Population Between IR64 and Oryza rufipogon. Developing blast resistance varieties with superior agronomical performance has been the one of the important priorities in rice breeding program. Based on the purpose of this study the double haploid and backcross populations were developed using the most popular cultivar IR64 as recurrent parent and wild rice species Oryza rufipogon (Acc. IRGC 105491) as blast resistance donor parent. This study was initiated to analyze the blast resistance and agronomical performance of double haploid populations (DH_I, DH_II and DH_III) and backcross populations (BC2, BC3, and BC5), based on the green house and field screening tests. The results of statistical analysis showed that the blast resistance performance of DH population were diverse among DH_I, DH_II and DH_III. The smallest diversity was on the DH_III population. The same results were also detected on BC populations. The smallest diversity was on BC5 population. The diversity comparison between DH and BC population showed that DH_III population had smaller variation than BC5. Indicated that DH_III population has the most fixed population. The agronomic performance evaluation of DH_III population selected lines showed that Bio1, Bio2, and Bio8 qualitified as the candidate of promising lines. AbstrakPerakitan varietas tahan blas sebagai galur harapan, merupakan salah satu prioritas dalam program pemuliaan padi. Dalam rangka mendukung program tersebut, telah dilakukan pembentukan populasi haploid ganda (HG) dan silang balik (BC) dengan IR64 sebagai tetua berulang dan Oryza rufipogon (No. aksesi IRGC 105491) sebagai tetua donor gen tahan penyakit blas. Penelitian ini bertujuan menganalisis keragaan tingkat ketahanan galur-galur haploid ganda (HG_I, HG_II, dan HG_III) dan galur-galur silang balik (BC2, BC3, dan BC5) terhadap penyakit blas di rumah kaca dan lapang, sehingga diperoleh kandidat galur harapan. Hasil pengujian beberapa populasi HG dan BC menunjukan bahwa terdapat variasi keragaan yang berbeda-beda. Variasi paling kecil terdapat pada populasi HG_III. Hasil yang sama juga diperoleh pada populasi silang balik (BC2-BC5). Variasi paling kecil terdapat pada populasi BC5. Bila dibandingkan antar populasi HG dan BC, tingkat variasi pada populasi HG_III lebih kecil dibandingkan dengan tingkat variasi pada populasi BC5. Hal ini menunjukkan bahwa tingkat homosigositas paling tinggi terdapat pada populasi HG_III. Berdasarkan evaluasi penampilan agronomis beberapa galur HG_III terpilih, diperoleh tiga galur kandidat galur harapan Bio1, Bio2, dan Bio8.

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Identification of rice sheath blight and blast quantitative trait loci in two different O. sativa/O. nivara advanced backcross populations

Molecular Breeding ◽

10.1007/s11032-013-9843-y ◽

2013 ◽

Vol 31 (4) ◽

pp. 889-907 ◽

Cited By ~ 26

Author(s):

G. C. Eizenga ◽

B. Prasad ◽

A. K. Jackson ◽

M. H. Jia

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Sheath Blight ◽

Rice Sheath Blight ◽

Advanced Backcross ◽

Trait Loci ◽

Backcross Populations

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Large-scale density-based screening for pea weevil resistance in advanced backcross lines derived from cultivated field pea (Pisum sativum) and Pisum fulvum

Crop and Pasture Science ◽

10.1071/cp12225 ◽

2012 ◽

Vol 63 (7) ◽

pp. 612 ◽

Cited By ~ 23

Author(s):

N. Aryamanesh ◽

O. Byrne ◽

D. C. Hardie ◽

T. Khan ◽

K. H. M. Siddique ◽

...

Keyword(s):

Pisum Sativum ◽

Large Scale ◽

Interspecific Cross ◽

Field Pea ◽

Separation Method ◽

Density Separation ◽

Advanced Backcross ◽

Pisum Fulvum ◽

Resistant Lines ◽

Backcross Populations

The pea weevil, Bruchus pisorum, is one of the most intractable pest problems of cultivated field pea (Pisum sativum) in the world. Pesticide application, either as a contact insecticide spray to the field pea crop or fumigation of the harvested seed, is the only available method for its control. The aim of the study was to develop a quick and reliable method to screen for pea weevil resistance and increase efficiency in breeding for this important trait. Backcrossed progenies derived from an interspecific cross between cultivated field pea and its wild relative (Pisum fulvum, source of resistance for pea weevil) were subjected to natural infestation in field plots. Mature seeds were hand-harvested, stored to allow development of adult beetles, and then separated into infested and non-infested using a density separation method in 30% caesium chloride (CsCl). Susceptibility and resistance of the progenies were calculated based on this method and further confirmed by a glasshouse bioassay. Resistance in backcross populations improved considerably through selection of resistant lines using the density separation method. We found that the method using CsCl separation is a useful tool in breeding for pea weevil resistance. We were able to introgress pea weevil resistance from P. fulvum into cultivated field pea through backcrossing to produce several advanced pea weevil resistant lines following this procedure.

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