Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato

Soil salinity is one of the major environmental stresses that restrict the growth and development of tomato (Solanum lycopersicum L.) worldwide. In Arabidopsis, the calcium signaling pathway mediated by calcineurin B-like protein 4 (CBL4) and CBL-interacting protein kinase 24 (CIPK24) plays a critical role in salt stress response. In this study, we identified and isolated two tomato genes similar to the Arabidopsis genes, designated as SlCBL4 and SlCIPK24, respectively. Bimolecular fluorescence complementation (BiFC) and pull-down assays indicated that SlCBL4 can physically interact with SlCIPK24 at the plasma membrane of plant cells in a Ca2+-dependent manner. Overexpression of SlCBL4 or superactive SlCIPK24 mutant (SlCIPK24M) conferred salt tolerance to transgenic tomato (cv. Moneymaker) plants. In particular, the SlCIPK24M-overexpression lines displayed dramatically enhanced tolerance to high salinity. It is notable that the transgenic plants retained higher contents of Na+ and K+ in the roots compared to the wild-type tomato under salt stress. Taken together, our findings clearly suggest that SlCBL4 and SlCIPK24 are functional orthologs of the Arabidopsis counterpart genes, which can be used or engineered to produce salt-tolerant tomato plants.

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Faculty Opinions recommendation of The structure of the Arabidopsis thaliana SOS3: molecular mechanism of sensing calcium for salt stress response.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1024162.286767 ◽

2005 ◽

Author(s):

Ramón Serrano

Keyword(s):

Arabidopsis Thaliana ◽

Salt Stress ◽

Stress Response ◽

Molecular Mechanism ◽

Salt Stress Response

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Progress in understanding salt stress response in plants using biotechnological tools

Journal of Biotechnology ◽

10.1016/j.jbiotec.2021.02.007 ◽

2021 ◽

Vol 329 ◽

pp. 180-191

Author(s):

Ulkar İbrahimova ◽

Pragati Kumari ◽

Saurabh Yadav ◽

Anshu Rastogi ◽

Michal Antala ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Salt Stress Response

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Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii

BMC Genomics ◽

10.1186/1471-2164-13-215 ◽

2012 ◽

Vol 13 (1) ◽

pp. 215 ◽

Cited By ~ 51

Author(s):

Guido Mastrobuoni ◽

Susann Irgang ◽

Matthias Pietzke ◽

Heike E Aßmus ◽

Markus Wenzel ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Chlamydomonas Reinhardtii ◽

Salt Stress Response ◽

Photosynthetic Organism

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Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana

Molecular and Cellular Biology ◽

10.1128/mcb.00429-07 ◽

2007 ◽

Vol 27 (22) ◽

pp. 7771-7780 ◽

Cited By ~ 122

Author(s):

Paul E. Verslues ◽

Giorgia Batelli ◽

Stefania Grillo ◽

Fernanda Agius ◽

Yong-Sig Kim ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Stress Responses ◽

Double Mutant ◽

Nucleoside Diphosphate Kinase ◽

Salt Resistance ◽

Interacting Proteins ◽

Salt Stress Response ◽

Oxygen Signaling ◽

Nucleoside Diphosphate Kinase 2

ABSTRACT SOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and stress signaling through its interactions with proteins involved in membrane transport and in regulation of stress responses. We have identified additional SOS2-interacting proteins that suggest a connection between SOS2 and reactive oxygen signaling. SOS2 was found to interact with the H2O2 signaling protein nucleoside diphosphate kinase 2 (NDPK2) and to inhibit its autophosphorylation activity. A sos2-2 ndpk2 double mutant was more salt sensitive than a sos2-2 single mutant, suggesting that NDPK2 and H2O2 are involved in salt resistance. However, the double mutant did not hyperaccumulate H2O2 in response to salt stress, suggesting that it is altered signaling rather than H2O2 toxicity alone that is responsible for the increased salt sensitivity of the sos2-2 ndpk2 double mutant. SOS2 was also found to interact with catalase 2 (CAT2) and CAT3, further connecting SOS2 to H2O2 metabolism and signaling. The interaction of SOS2 with both NDPK2 and CATs reveals a point of cross talk between salt stress response and other signaling factors including H2O2.

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