Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic sites, the defect in repair enzymes led to a decrease of phage survival and a total absence of bacterial DNA degradation and of phage DNA synthesis. These results directly demonstrate the toxic action of apurinic sites on bacteriophage T7 at the intracellular level and its alleviation by DNA repair. In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant.Key words: apurinic sites, DNA repair, T7 phage.

Download Full-text

RNA polymerase synthesis in vitro directed by T7 phage DNA

MGG Molecular & General Genetics ◽

10.1007/bf00276043 ◽

1971 ◽

Vol 110 (1) ◽

pp. 31-35 ◽

Cited By ~ 10

Author(s):

Peter Herrlich ◽

Manfred Schweiger

Keyword(s):

Rna Polymerase ◽

Phage Dna ◽

T7 Phage

Download Full-text

In vitro transcription assay of ozonated T7 phage dna

Environmental and Molecular Mutagenesis ◽

10.1002/em.2850160108 ◽

1990 ◽

Vol 16 (1) ◽

pp. 44-47 ◽

Cited By ~ 5

Author(s):

Catherine Mura ◽

Young Sup Chung ◽

E. R. Nestman

Keyword(s):

In Vitro Transcription ◽

Transcription Assay ◽

Phage Dna ◽

T7 Phage

Download Full-text

Relative roles of T7 RNA polymerase and gene 4 primase for the initiation of T7 phage DNA replication in vivo.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.84.12.3977 ◽

1987 ◽

Vol 84 (12) ◽

pp. 3977-3981 ◽

Cited By ~ 9

Author(s):

K. Sugimoto ◽

Y. Kohara ◽

T. Okazaki

Keyword(s):

Dna Replication ◽

Rna Polymerase ◽

T7 Rna Polymerase ◽

Phage Dna ◽

T7 Phage ◽

Gene 4

Download Full-text

Kinetic and spectrophotometric studies on the renaturation of deoxyribonucleic acid

Biochemical Journal ◽

10.1042/bj1090543 ◽

1968 ◽

Vol 109 (4) ◽

pp. 543-557 ◽

Cited By ~ 18

Author(s):

K. J. Thrower ◽

A. R. Peacocke

Keyword(s):

Ionic Strength ◽

Rate Constant ◽

Base Pairs ◽

Phage Dna ◽

Dna Strands ◽

Entropy Of Activation ◽

T7 Phage ◽

Kinetics Of ◽

Rate Controlling Step ◽

Adenine Thymine

The kinetics of the renaturation of Escherichia coli DNA in 0·4–1·0m-sodium chloride at temperatures from 60° to 90° have been studied. The extent of renaturation was a maximum at 65° to 75° and increased with ionic strength, and the rate constant increased with both ionic strength and temperature. The energy and entropy of activation of renaturation were calculated to be 6–7kcal.mole−1 and −40cal.deg.−1mole−1 respectively. It has been shown that renaturation is a second-order process for 5hr. under most conditions. The results are consistent with a reaction in which the rate-controlling step is the diffusion together of two separated complementary DNA strands and the formation of a nucleus of base pairs between them. The kinetics of the renaturation of T7-phage DNA and Bordetella pertussis DNA have also been studied, and their rates of renaturation related quantitatively to the relative heterogeneity of the DNA samples. By analysis of the spectra of DNA at different stages during renaturation it was shown that initially the renatured DNA was rich in guanine–cytosine base pairs and non-random in base sequence, but that, as equilibrium was approached, the renatured DNA gradually resembled native DNA more closely. The rate constant for the renaturation of guanine–cytosine base pairs was slightly higher than for adenine–thymine base pairs.

Download Full-text

Plastid DNA polymerases from higher plants, Arabidopsis thaliana

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2005.06.052 ◽

2005 ◽

Vol 334 (1) ◽

pp. 43-50 ◽

Cited By ~ 30

Author(s):

Yoko Mori ◽

Seisuke Kimura ◽

Ai Saotome ◽

Nobuyuki Kasai ◽

Norihiro Sakaguchi ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Higher Plants ◽

Dna Polymerases ◽

Plastid Dna

Download Full-text

An Endodeoxyribonuclease Induced after Infection with Phage T7 Bearing Amber Mutations in Genes 3, 5, and 6

Canadian Journal of Biochemistry ◽

10.1139/o72-140 ◽

1972 ◽

Vol 50 (9) ◽

pp. 1016-1023 ◽

Cited By ~ 10

Author(s):

Paul D. Sadowski

Keyword(s):

E Coli ◽

Phage Dna ◽

Phage T7 ◽

T7 Phage

An endodeoxyribonuclease activity was detectable in extracts from E. coli 1200 (su−, Endo I−) cells infected with T7 phage bearing amber mutations in genes 3, 5, and 6. The enzyme was purified 270-fold and it makes predominantly single-stranded breaks in native DNA. The breaks have 3′-hydroxyl and 5′-phosphoryl termini and the endonuclease acts almost as well on denatured DNA as on native DNA. The function of the enzyme is not known but its activity does not appear to be controlled by genes involved in replication or maturation of phage DNA.

Download Full-text