scholarly journals Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Li-Min Xie ◽  
Geng-Ling Lin ◽  
Hao-Neng Dong ◽  
Ying-Xia Liao ◽  
Ye-Ling Liu ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunochromatographic Assay

scholarly journals Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

2019 ◽  
Author(s):  
Li-Min Xie ◽  
Geng-ling Lin ◽  
Hao-Neng Dong ◽  
Ying-Xia Liao ◽  
Ye-Ling Liu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Pathogenic Fungus ◽  
High Specificity ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Cochrane Library ◽  
Specificity And Sensitivity ◽  
Accuracy Study ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background : Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens. Methods : Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias. Results : A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively. Conclusions : Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

scholarly journals Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

2020 ◽  
Author(s):  
Li-Min Xie ◽  
Geng-ling Lin ◽  
Hao-Neng Dong ◽  
Ying-Xia Liao ◽  
Ye-Ling Liu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Pathogenic Fungus ◽  
High Specificity ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Cochrane Library ◽  
Specificity And Sensitivity ◽  
Accuracy Study ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens. Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

scholarly journals Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

2020 ◽  
Author(s):  
Li-Min Xie ◽  
Geng-ling Lin ◽  
Hao-Neng Dong ◽  
Ying-Xia Liao ◽  
Ye-Ling Liu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Pathogenic Fungus ◽  
High Specificity ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Cochrane Library ◽  
Specificity And Sensitivity ◽  
Accuracy Study ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens. Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias. Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively. Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

scholarly journals Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

2019 ◽  
Author(s):  
Li-Min Xie ◽  
Geng-ling Lin ◽  
Hao-Neng Dong ◽  
Ying-Xia Liao ◽  
Ye-Ling Liu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Pathogenic Fungus ◽  
High Specificity ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Cochrane Library ◽  
Specificity And Sensitivity ◽  
Accuracy Study ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens. Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

scholarly journals Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiayi Wang ◽  
Jinyu Zhou ◽  
Yiqiang Chen ◽  
Xinpei Zhang ◽  
Yongpeng Jin ◽  
...  
Keyword(s):  
Animal Feed ◽  
Animal Production ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Animal Origin ◽  
Quantitative Detection ◽  
One Step ◽  
Assay Time ◽  
Animal Origin Food ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background Colistin (polymyxin E) is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention, treatment, and growth promotion. However, the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings, and its residue in animal-origin food may also pose serious health hazards to humans. Thus, it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food. Results A one-step enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic assay (LFIA) for colistin were developed based on a newly developed monoclonal antibody. The ELISA showed a 50% inhibition value (IC50) of 9.7 ng/mL with assay time less than 60 min, while the LFIA had a strip reader-based detection limit of 0.87 ng/mL in phosphate buffer with assay time less than 15 min. For reducing the non-specific adsorption of colistin onto sample vial, the components of sample extraction solution were optimized and proved to greatly improve the assay accuracy. The spiked recovery experiment showed that the recoveries of colistin from feed, milk and meat samples were in the range of 77.83% to 113.38% with coefficient of variations less than 13% by ELISA analysis and less than 18% by LFIA analysis, respectively. Furthermore, actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental analysis. Conclusions The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food.

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