scholarly journals An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical precision testing

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ling Deng ◽  
Xiao-Yi He ◽  
Bin Tang ◽  
Yang Xiang ◽  
Juan-Juan Yue
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Real Time ◽  
Chain Reaction ◽  
Stomach Tissue ◽  
Polymerase Chain Reaction Technology ◽  
Polymerase Chain ◽  
Precision Testing

Helicobacter pylori clarithromycin resistance detected by Etest and TaqMan real-time polymerase chain reaction: a comparative study

Apmis ◽  
2012 ◽  
Vol 120 (9) ◽  
pp. 712-717 ◽  
Author(s):  
Rosa Monno ◽  
Floriana Giorgio ◽  
Panella Carmine ◽  
Leonardo Soleo ◽  
Vittoria Cinquepalmi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Comparative Study ◽  
Real Time ◽  
Chain Reaction ◽  
Clarithromycin Resistance ◽  
Polymerase Chain

scholarly journals Utility of normalized genome quantification of Helicobacter pylori in gastric mucosa using an in-house real-time polymerase chain reaction

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178674 ◽  
Author(s):  
Ana Morilla ◽  
Santiago Melón ◽  
Marta E. Álvarez-Argüelles ◽  
Edisa Armesto ◽  
Henar Villar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Clarithromycin resistance assessed on helicobacter pylori dna sequences by a novel real time polymerase chain reaction

2006 ◽  
Vol 38 ◽  
pp. S39-S40
Author(s):  
V. De Francesco ◽  
E. Ierardi ◽  
M. Margiotta ◽  
A. Zullo ◽  
C. Hassan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Real Time ◽  
Dna Sequences ◽  
Chain Reaction ◽  
Clarithromycin Resistance ◽  
Polymerase Chain

scholarly journals Refluks Helicobacter pylori di mukosa hidung penderita rinosinusitis kronik disertai refluks laringofaring

2019 ◽  
Vol 48 (2) ◽  
pp. 148
Author(s):  
Sinta Sari Ratunanda ◽  
Billy Talakua ◽  
Teti Madiadipoera ◽  
Thaufiq Boesoirie ◽  
Ratna Anggraeni ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Real Time ◽  
Chronic Rhinosinusitis ◽  
Nasal Mucosa ◽  
Nasal Discharge ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Polymerase Chain ◽  
H Pylori

Latar belakang: Rinosinusitis kronik masih menjadi problema di seluruh dunia. Faktor yang berasosiasi dengan Rinosinusitis Kronik (RSK) diduga multifaktorial, salah satunya adalah refluks laringofaring (RLF). Isi refluks cairan lambung antara lain adalah bakteri Helicobacter pylori (H. pylori) yang dengan patomekanisme refluks, diduga dapat mencapai mukosa laringofaring bahkan sampai mukosa sinonasal, dan menyebabkan RSK. Tujuan: Mendeteksi  H. pylori di mukosa hidung akibat refluks pada penderita RSK disertai RLF. Bila terdeteksi H. pylori, tata laksana harus lebih komprehensif, sehingga diharapkan RSK menjadi terkontrol. Metode: Penelitian deskriptif untuk mengetahui ada tidaknya H. pylori di mukosa sinonasal penderita RSK dengan RLF. Deteksi H. pylori menggunakan teknik quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) dari bahan penyikatan mukosa hidung. Hasil: Didapatkan 86 orang penderita RSK disertai RLF, terdiri dari 30 (35%) pasien laki-laki dan 56 (65,0%) pasien wanita, dengan rerata usia 43,25±6,30 tahun. Keluhan RSK terbanyak adalah hidung tersumbat dengan skor VAS > 7 sebesar 76,8%. Skor nasoendoskopi RSK terbesar pada skor 2 untuk edema mukosa sebesar 65,3% dan skor 2 untuk sekret hidung sebesar 58,2%. Rata-rata skor gejala refluks (SGR) adalah 26,43±4,03 dan rata-rata total skor temuan refluks (STR) adalah 11,28±1,21. Hasil pemeriksaan deteksi H. pylori dengan qRT-PCR, 100% tidak menemukan H. pylori dari penyikatan mukosa hidung. Kesimpulan: Refluks berupa H. pylori tidak ditemukan pada mukosa hidung  penderita RSK disertai RLF. Penelitian lebih lanjut diperlukan dengan menggunakan gabungan beberapa metode pemeriksaan  bersamaan untuk deteksi H. pylori akibat refluks di mukosa sinonasal  penderita RSK disertai RLF.  Background: Chronic rhinosinusitis is presently still a worldwide problem. Assosiating factors  to chronic rhinosinusitis (CRS) are multifactorial, one of them is laryngopharyngeal reflux (LPR). The gastric juice contains Helicobacter pylori (H. pylori), which by pathologic reflux could reach laryngopharyngeal and sinonasal area causing CRS. Purpose: To detect H. pylori in nasal mucosa caused by reflux, which suspected of causing CRS with LPR disease. Should H. pylori be found in nasal mucosa, the management of the disease must be comprehensive to enable  controlling CRS. Methods: A descriptive study to detect H. pylori in nasal mucosa CRS with LPR patients, using Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) through nasal brushing. Results: Eighty-six CRS with LPR patients as study objects consisted of 30 (35%) male, and 56 (65%) female, the age mean was 43.25±6.3 years old. Visual Analoque Scale (VAS) score for nasal obstruction more than 7 was the highest complaint (76.8%). Nasal endoscopic score of mucosal edema (65.3%) and nasal discharge (58,2%) had score 2. The average total score reflux symptom index (RSI) was 26.43±4.03 and the total score reflux finding score (RFS) was 11.28±1.21. H. pylori detection found negative 100% in CRS with LPR specimens. Conclusion: This study did not find reflux containing H. pylori in nasal mucosa of CRS with LPR patients.    Suggesting further study using simultaneously several methods to detect H. pylori in nasal mucosa  CRS with LPR patients.

Sign in / Sign up

Export Citation Format

Share Document