Correction to: Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro

Abstract Background Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents. Methods To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human dental saliva and plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device. Results HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qRT-PCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions. Conclusion We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.

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Use of an Oxygen Planar Optode to Assess the Effect of High Velocity Microsprays on Oxygen Penetration in a Human Dental Biofilms In-Vitro

10.21203/rs.2.13863/v4 ◽

2020 ◽

Author(s):

Yalda Khosravi ◽

Raja Durga Prasad Kandukuri ◽

Sara Palmer ◽

Erin S Gloag ◽

Sergey M. Borisov ◽

...

Keyword(s):

High Velocity ◽

Individual Species ◽

Oxygen Penetration ◽

Copy Numbers ◽

16S Rna ◽

Fluid Jets ◽

Planar Optode ◽

Causative Agents ◽

The Impact

Abstract BackgroundDental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents.MethodsTo investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human dental saliva and plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device.ResultsHVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qRT-PCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions.ConclusionWe concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.

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Use of an Oxygen Planar Optode to Assess the Effect of High Velocity Microsprays on Oxygen Penetration in a Human Dental Biofilms In-Vitro

10.21203/rs.2.13863/v1 ◽

2019 ◽

Author(s):

Yalda Khosravi ◽

Raja Durga Prasad Kandukuri ◽

Sara Palmer ◽

Sergey M. Borisov ◽

Michelle Starke ◽

...

Keyword(s):

High Velocity ◽

Antimicrobial Agents ◽

Individual Species ◽

Oxygen Penetration ◽

16S Rna ◽

Fluid Jets ◽

Planar Optode ◽

Causative Agents ◽

The Impact

Abstract Background Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents. Methods To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human dental saliva and plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device. Results HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qRT-PCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions. Conclusion We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.

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Use of an Oxygen Planar Optode to Assess the Effect of High Velocity Microsprays on Oxygen Penetration in a Human Dental Biofilms In-Vitro

10.21203/rs.2.13863/v3 ◽

2020 ◽

Author(s):

Yalda Khosravi ◽

Raja Durga Prasad Kandukuri ◽

Sara Palmer ◽

Erin Samantha Gloag ◽

Sergey M. Borisov ◽

...

Keyword(s):

High Velocity ◽

Antimicrobial Agents ◽

Individual Species ◽

Oxygen Penetration ◽

16S Rna ◽

Fluid Jets ◽

Planar Optode ◽

Causative Agents ◽

The Impact

Abstract Background Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents. Methods To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human dental saliva and plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device. Results HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qRT-PCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions. Conclusion We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.

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Microstructural and in vitro characterisation of high-velocity suspension flame sprayed (HVSFS) bioactive glass coatings

Journal of the European Ceramic Society ◽

10.1016/j.jeurceramsoc.2009.01.032 ◽

2009 ◽

Vol 29 (11) ◽

pp. 2249-2257 ◽

Cited By ~ 31

Author(s):

G. Bolelli ◽

V. Cannillo ◽

R. Gadow ◽

A. Killinger ◽

L. Lusvarghi ◽

...

Keyword(s):

Bioactive Glass ◽

High Velocity

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Coronary stents cause high velocity fluctuation with a flow acceleration and flow reduction in jailed branches: An in vitro study using laser-Doppler anemometry

Biorheology ◽

10.3233/bir-2012-0617 ◽

2012 ◽

Vol 49 (5-6) ◽

pp. 329-340 ◽

Cited By ~ 6

Author(s):

Jakob Dörler ◽

Matthias Frick ◽

Monika Hilber ◽

Harald Breitfuss ◽

Mohammed N. Abdel-Hadi ◽

...

Keyword(s):

High Velocity ◽

Velocity Fluctuation ◽

Laser Doppler Anemometry ◽

In Vitro Study ◽

Coronary Stents ◽

Laser Doppler ◽

Vitro Study ◽

Flow Acceleration ◽

Flow Reduction

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Microstructure and in vitro behaviour of 45S5 bioglass coatings deposited by high velocity suspension flame spraying (HVSFS)

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-011-4307-6 ◽

2011 ◽

Vol 22 (5) ◽

pp. 1303-1319 ◽

Cited By ~ 36

Author(s):

L. Altomare ◽

D. Bellucci ◽

G. Bolelli ◽

B. Bonferroni ◽

V. Cannillo ◽

...

Keyword(s):

High Velocity ◽

Flame Spraying ◽

45S5 Bioglass

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Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.530 ◽

2011 ◽

Vol 493-494 ◽

pp. 530-534 ◽

Cited By ~ 1

Author(s):

Anke Bernstein ◽

Norbert Suedkamp ◽

Hermann Otto Mayr ◽

R. Gadow ◽

A. Killinger ◽

...

Keyword(s):

High Velocity ◽

Cell Attachment ◽

Ceramic Coatings ◽

In Vitro Cytotoxicity ◽

Cellular Growth ◽

Flame Spraying ◽

Biological Fixation ◽

Coating Materials ◽

Identical Cell

Bioceramics used as coatings show different biocompatibility and bioactive behavior in relation to their chemical and morphological behavior. Bioactive ceramics such as β-tricalcium phosphate (β-TCP) promote and enhance biological fixation. Stable coatings require an optimum between resorption rate, flexural strength and adhesive strength of the coating. Therefore new bioceramic coating materials that ensure the balance between loss of substances and osteointegration need to be designed and investigated. By modifying the high velocity suspension flame spraying (HVSFS) process parameters, five coatings with different materials were obtained. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay after 24, 48 and 72 h. Cells were grown on the materials for 3, 7, 14, and 21 days and counted. Cell morphology, cell attachment, and cell spreading were investigated using fluorescence microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified.

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In Vitro Evaluation of Biologically Derived Hydroxyapatite Coatings Manufactured by High Velocity Suspension Spraying

Journal of Thermal Spray Technology ◽

10.1007/s11666-021-01265-0 ◽

2021 ◽

Author(s):

M. Blum ◽

M. Sayed ◽

E. M. Mahmoud ◽

A. Killinger ◽

R. Gadow ◽

...

Keyword(s):

High Velocity ◽

Inductively Coupled Plasma ◽

Ph Value ◽

Low Cost ◽

Optical Emission ◽

Fish Bone ◽

Emission Spectrometry ◽

Slight Variation ◽

Flame Spray

AbstractThis investigation aims to study a novel biologically derived coating applied on Ti alloy substrates. Obtained from a low-cost fish bone resource, a nanocrystalline hydroxyapatite has been synthesized and converted to an organic suspension. Coating was then manufactured by a high-velocity suspension flame spray process. The microstructure, phase composition, coating thickness, and roughness of hydroxyapatite (HA)-coated samples were studied. The results indicated the presence of both hydroxyapatite and β-tricalcium phosphate phases and the final coating layer was uniform and dense. In vitro bioactivity and biodegradability of the HA/Ti composite samples were estimated by immersion in simulated body fluid. Remarkable reductions in Ca2+ and PO43− ion concentrations were observed as well as low weight loss percentage and a slight variation in the pH value, indicating the generation of an apatite layer on the surface of all studied samples. Scanning electron microscopy, energy-dispersive x-ray analysis, and inductively coupled plasma–optical emission spectrometry confirm these results. Thus biological derived HA coatings are a promising candidate to enhance bioactivity and biodegradability of bone implants. To demonstrate feasibility on commercial medical components, a medical screw was coated and evaluated.

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