scholarly journals Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Qian Wang ◽  
Yongxin Wang ◽  
Silin Pang ◽  
Jia Zhou ◽  
Jie Cai ◽  
...  
Keyword(s):  
Substance P ◽  
Growth Inhibition ◽  
Hair Follicle ◽  
Nerve Fibers ◽  
Follicle Growth ◽  
Alcohol Extract ◽  
Hair Follicle Growth ◽  
Vernonia Anthelmintica

Abstract Background Vernonia anthelmintica (L.) willd is a traditional urgur herb in China for a long history. Its alcohol extract (AVE) has been proved to promote hair follicle growth in C57BL/6 mice. We conducted this study to investigate the hair-growth effects of AVE in stressed mice and its possible mechanism of action. Methods The hair-follicle growth effects of AVE were examined by in vivo and in vitro study. We exposed C57BL/6 male mice to chronic restraint stress to induce murine hair follicle growth inhibition. The effects of AVE were examined by histological analysis, immunofluorescence for Ki67 and cytokeratin 19 immunoreactivity, western blot assay in tyrosinase and related proteins expressions and immunofluorescence for nerve fibers. In organ culture of mouse vibrissae follicles, we used substance P as a catagen-inducing factor of hair follicle growth, and measured the elongation of hair shafts and expression of neurokinin-1 receptor protein by application of AVE. Results Our results showed that AVE counteract murine hair follicle growth inhibition caused by chronic restraint stress via inducing the conversion of telogen to anagen and inhibiting catagen premature, increasing bulb keratinocytes and bulge stem cells proliferation, promoting melanogenesis, and reducing the numbers of substance P and calcitonin gene-related peptide nerve fibers. Furthermore, AVE also counteracted murine hair follicle growth inhibition caused by substance P in organ culture. Conclusion These results suggest that AVE counteract stress-induced hair follicle growth inhibition in C57BL/6 mice in vivo and in vitro, and may be an effective new candidate for treatment of stress-induced hair loss.

scholarly journals Minoxidil-Coated Lysozyme-Shelled Microbubbes Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo

2021 ◽  
Vol 12 ◽  
Author(s):  
Ai-Ho Liao ◽  
Yu-Jhen Huang ◽  
Ho-Chiao Chuang ◽  
Chih-Hung Wang ◽  
Cheng-Ping Shih ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Animal Experiments ◽  
Follicle Cell ◽  
Follicle Cells ◽  
Follicle Growth ◽  
Dermal Papilla Cells ◽  
The Us ◽  
Hair Follicle Growth

Lysozyme (Lyz) is an antimicrobial peptide, a safe adjunct, and it has been indicated that Lyz can promote vibrissae follicle growth by enhancing the hair-inductive capacity of dermal papilla cells in mice. The present study produced a new type of minoxidil (Mx)-coated antifungal Lyz-shelled microbubble (LyzMB) for inhibiting bacteria and allergies on the oily scalp. The potential of Mx-coated LyzMBs (Mx-LyzMBs) combined with ultrasound (US) and the role of LyzMB fragments in enhancing hair follicle growth were investigated. Mx grafted with LyzMBs were synthesized and the loading efficiency of Mx on cationic LyzMBs was 20.3%. The biological activity of Lyz in skin was determined using an activity assay kit and immunohistochemistry expression, and the activities in the US+Mx-LyzMBs group were 65.8 and 118.5 μU/mL at 6 and 18 h, respectively. In hair follicle cell culture experiments, the lengths of hair follicle cells were significantly enhanced in the US+Mx-LyzMBs group (108.2 ± 11.6 μm) compared to in the US+LyzMBs+Mx group (44.3 ± 9.8 μm) and the group with Mx alone (79.6 ± 12.0 μm) on day 2 (p < 0.001). During 21 days of treatment in animal experiments, the growth rates at days 10 and 14 in the US+Mx-LyzMBs group increased by 19.4 and 65.7%, respectively, and there were significant differences (p < 0.05) between the US+Mx-LyzMBs group and the other four groups. These findings indicate that 1-MHz US (applied at 3 W/cm2, acoustic pressure = 0.266 MPa) for 1 min combined with Mx-LyzMBs can significantly increase more penetration of Mx and LyzMB fragments into skin and enhance hair growth than Mx alone.

Rat hair follicle growth in vitro

1992 ◽  
Vol 127 (6) ◽  
pp. 600-607 ◽  
Author(s):  
M.P. PHILPOTT ◽  
M.R. GREEN ◽  
T. KEALEY
Keyword(s):  
Hair Follicle ◽  
Follicle Growth ◽  
Hair Follicle Growth

Controls of Hair Follicle Cycling

2001 ◽  
Vol 81 (1) ◽  
pp. 449-494 ◽  
Author(s):  
K. S. Stenn ◽  
R. Paus
Keyword(s):  
Growth Factor ◽  
Hair Follicle ◽  
Cell Biology ◽  
Transforming Growth Factor ◽  
Gene Families ◽  
Transforming Growth Factor Β ◽  
Follicle Growth ◽  
Modern Biology ◽  
Hair Follicle Growth

Nearly 50 years ago, Chase published a review of hair cycling in which he detailed hair growth in the mouse and integrated hair biology with the biology of his day. In this review we have used Chase as our model and tried to put the adult hair follicle growth cycle in perspective. We have tried to sketch the adult hair follicle cycle, as we know it today and what needs to be known. Above all, we hope that this work will serve as an introduction to basic biologists who are looking for a defined biological system that illustrates many of the challenges of modern biology: cell differentiation, epithelial-mesenchymal interactions, stem cell biology, pattern formation, apoptosis, cell and organ growth cycles, and pigmentation. The most important theme in studying the cycling hair follicle is that the follicle is a regenerating system. By traversing the phases of the cycle (growth, regression, resting, shedding, then growth again), the follicle demonstrates the unusual ability to completely regenerate itself. The basis for this regeneration rests in the unique follicular epithelial and mesenchymal components and their interactions. Recently, some of the molecular signals making up these interactions have been defined. They involve gene families also found in other regenerating systems such as fibroblast growth factor, transforming growth factor-β, Wnt pathway, Sonic hedgehog, neurotrophins, and homeobox. For the immediate future, our challenge is to define the molecular basis for hair follicle growth control, to regenerate a mature hair follicle in vitro from defined populations, and to offer real solutions to our patients' problems.

CRH peptides modulate human hair follicle growth in vitro- a preliminary study

2008 ◽  
Vol 13 (9) ◽  
pp. 582-583
Author(s):  
S. Kauser ◽  
A. Slominski ◽  
E. T. Wei ◽  
D. J. Tobin
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Follicle Growth ◽  
Human Hair Follicle ◽  
Preliminary Study ◽  
Hair Follicle Growth

Antimicrobial peptide lysozyme has the potential to promote mouse hair follicle growth in vitro

2015 ◽  
Vol 117 (8) ◽  
pp. 798-802 ◽  
Author(s):  
Yongsheng Su ◽  
Hui Liu ◽  
Jin Wang ◽  
Bojie Lin ◽  
Yong Miao ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Hair Follicle ◽  
Follicle Growth ◽  
Hair Follicle Growth

scholarly journals A question for scientists in tissue physiology.

2018 ◽  
Author(s):  
Stephen Foote
Keyword(s):  
Human Physiology ◽  
Hair Follicle ◽  
Normal Tissue ◽  
Tissue Growth ◽  
Hair Follicles ◽  
Follicle Growth ◽  
Spatial Growth ◽  
Hair Follicle Growth ◽  
Growth Controls

Current research into the often restricted growth of hair follicles, fails to take account of the spatial growth controls that govern all such normal tissue growth in-vivo. Spatial growth controls in this context, have wider implications in Human physiology and disease. So I wish to ask scientists if there is evidence that hair follicle growth is an exception to the rule here?

scholarly journals Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods

2020 ◽  
Vol 33 (5) ◽  
pp. 280-292
Author(s):  
Ehsan Taghiabadi ◽  
Mohammad Ali Nilforoushzadeh ◽  
Nasser Aghdami
Keyword(s):  
Hair Follicle ◽  
Dermal Papilla ◽  
Culture Conditions ◽  
Hair Follicles ◽  
Main Role ◽  
Follicle Growth ◽  
In Vitro Morphogenesis ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth

The dermal papilla comprises mesenchymal cells in hair follicles, which play the main role in regulating hair growth. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) and dermal sheath cells during cell culture is the main factor in in vitro morphogenesis and regeneration of hair follicles. Using common methods for the cultivation of human dermal papilla reduces the maintenance requirements of the inductive capacity of the dermal papilla and the expression of specific dermal papilla biomarkers. Optimizing culture conditions is therefore crucial for DPCs. Moreover, exosomes appear to play a key role in regulating the hair follicle growth through a paracrine mechanism and provide a functional method for treating hair loss. The present review investigated the biology of DPCs, the molecular and cell signaling mechanisms contributing to hair follicle growth in humans, the properties of the dermal papilla, and the effective techniques in maintaining hair inductivity in DPC cultures in humans as well as hair follicle bioengineering.

Substance P decreases fluid absorption in the renal proximal tubule

1997 ◽  
Vol 272 (5) ◽  
pp. R1396-R1401 ◽  
Author(s):  
R. D. Fildes ◽  
J. L. Atkins
Keyword(s):  
Nitric Oxide ◽  
Substance P ◽  
Proximal Tubule ◽  
Nerve Fibers ◽  
The Body ◽  
Afferent Nerve ◽  
Fluid Absorption ◽  
Organ Systems

Substance P is a neuropeptide found principally in the central nervous system and peripheral afferent nerve fibers. It is widely distributed in the body, and its local release is thought to have important effects in the normal physiology and pathophysiology of several organ systems. Substance P releases histamine from mast cells and nitric oxide from endothelial cells. Systemic infusion of substance P causes a brisk natriuresis. Previously, proximal tubule transport in the kidney was measured in vivo during the infusion of substance P. Transport was inhibited. This could have been a direct action of substance P or secondary to the release of histamine or nitric oxide. Therefore, we have now studied the effect of substance P on isolated proximal tubules perfused in vitro. We found that 10(-10) M substance P caused a 50% reduction in the rate of fluid absorption in rabbit proximal straight tubule. This effect was reversible on removal of substance P from the bath. Inhibition of nitric oxide production with NG-monomethyl-L-arginine (10(-4) M) did not influence the action of substance P at 10(-10) M. The sensitivity of the proximal tubule to low concentrations of substance P, together with the recent findings of substance P-containing afferent fibers within the cortex of the kidney, suggest a role for substance P in the control of proximal tubule reabsorption, particularly in pathophysiological conditions associated with increased afferent nerve activity, such as ureteric occlusion.

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