scholarly journals Validation and quantification of major biomarkers in ‘Mahasudarshan Churna’- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Prabhjot Kaur ◽  
R. C. Gupta ◽  
Abhijit Dey ◽  
Tabarak Malik ◽  
Devendra Kumar Pandey
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Polyherbal Formulation ◽  
Validation And Quantification ◽  
Layer Chromatography

scholarly journals LUPEOL VALIDATION AND QUANTIFICATION IN HETEROPOGON CONTORTUS (L.) BEAUV. (SPEAR GRASS) THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2017 ◽  
Vol 10 (12) ◽  
pp. 392 ◽  
Author(s):  
Navjot Kaur ◽  
Raghbir Chand Gupta
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Marker Compound ◽  
Heteropogon Contortus ◽  
Methanolic Extracts ◽  
Validation And Quantification ◽  
Two Samples ◽  
Layer Chromatography

Objective: This is aimed to study the chromatographic evaluation of triterpenoid, i.e., lupeol from methanolic extracts of leaves, stem, and inflorescence of Heteropogon contortus.Methods: The high-performance thin-layer chromatography (HPTLC) densitometry determination of lupeol was performed using optimized mobile phase toluene:methanol:formic acid (7:3:0.3 v/v) with a derivatization of freshly prepared anisaldehyde-sulfuric acid. For densitometry measurements, the plates were scanned at 530 nm absorbance/reflectance wavelength. Quantification of lupeol marker compound in H. contortus leaves, stem, and inflorescence is estimated using 2-12 μg/spot.Results: The appearance of light purple bands on the chromatograms confirmed the lupeol component in plant samples. Further, the confirmation of the compound is done from the densitometric scanning by comparing λmax values. From this, it is reported that lupeol is present in leaf samples, i.e., 10 mg/g of dry wt., while in rest of the two samples, it is found absent.Conclusion: The leaves of H. contortus (spear grass) are a good source of lupeol and can be used as an alternate natural source to synthesize herbal drugs to control cancer and other anti-inflammatory agents. The presently selected HPTLC is validated and most accurate for the quantification and identification of lupeol present in the selected plant. The leaves of the species which are rich in lupeol can be used in pharmaceutical industry.

scholarly journals Physico-chemical investigation of a polyherbal formulation - Vidangatandulaadi choorna

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1728-1732
Author(s):  
Amrutha S. Babu ◽  
Mahadevan Subramaniam ◽  
Mahesh C. Kundagol ◽  
James Chacko ◽  
Devipriya Soman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Chemical Study ◽  
Inductively Coupled ◽  
Polyherbal Formulation ◽  
Physico Chemical ◽  
Plasma Mass Spectrometry ◽  
Layer Chromatography

Vidangatandulaadi Choorna is a polyherbal formulation consisting of seven ingredients. Trivrith (Operculina turpethum (Linn.) Silva Manso)  is the chief ingredient responsible for the purgative action of the formulation. This yoga is mentioned in Kalpasthana of Ashtangahridaya, intended for virechana (Purgation). It is useful in Kapha-vatha disorders. Even though many kinds of research have been done to identify the physicochemical constituents of individual drugs in the formulation, no studies were done to identify the physicochemical properties of the formulation. This analysis helps in understanding the mechanism for different pharmacological actions of the formulation. Hence, Physico-chemical study of Vidangatandulaadi Choorna along with high-performance thin-layer chromatography (HPTLC) fingerprinting is done to fix the standards. All the drugs included in the formulation is identified by the botanist and is prepared according to Standards mentioned for the preparation of Choorna mentioned in Ayurvedic Pharmacopoeia of India. The formulation is least encountered, but it has shown its significant action in Dyslipidemia in folklore practices. As there are no Standards mentioned for this formulation, the result observed in the present study may be considered suitable. The data obtained from Physicochemical investigation, high-performance thin-layer chromatography profile and ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) could be used as the standards for the present formulation under study.

scholarly journals STANDARDIZATION OF ANTICONVULSANT POLYHERBAL FORMULATION BY PHARMACOGNOSTICAL, DNA FINGERPRINTING AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY COMBINED WITH CHEMOMETRICS

2016 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Hetal Janani ◽  
Jayanta Kumar Maji ◽  
Ashish Verma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Dna Fingerprinting ◽  
High Performance ◽  
Retardation Factor ◽  
Plant Materials ◽  
Polyherbal Formulation ◽  
Diagnostic Characteristics ◽  
Microscopic Evaluation ◽  
Layer Chromatography

Objective: The combination between the stem bark powder of Chirabilva and Veerataru is used ethno folklore for the management of anti-convulsion by tribal people. To standardise the evidently used polyherbal formulation in a systemic way for supporting the identity, quality, purity, safety and efficacy concern with modern technique.Methods: Microscopic anatomical examination and powder microscopy were performed fresh and dried plant materials respectively. The compound polyherbal formulation is processed by organoleptic characterization, macro-microscopic evaluation, physicochemical, phytochemical testing, DNA fingerprinting and high-performance thin-layer chromatography (HPTLC) profiling employing a standard methodology. Chromatographic fingerprinting after visualisation the data are exploited by multivariate chemometric technique.Results: Results of the experiment provided diagnostic characteristics to identify quality and purity and standardise the polyherbal formulation along with respective ingredients. The RAPD analysis of Dichrostachys cinerea and Holoptelea integrin folia showed some similar bands at the same base pair indicate the presence of genetical identity may take into consideration of the control the ingredients. HPTLC technique utilised to distinguish the ingredient and polyherbal formulation based on the presence or absence of certain target phytochemical (flavonoid, polyphenol, etc.) constituents, manifested as peaks or bands from the chemical fingerprint profiles. Visualized chromatographic profile of polyherbal formulation along with its constituents applying the multivariate chemometric technique is easily discriminated of respective retardation factor in principal component score space.Conclusion: The findings from this study will provide systemic evaluation for this anticonvulsant formulation and also serve as a master document to control the quality of polyherbal formulation.Keywords: Anticonvulsant, Chemometrics, DNA fingerprinting, HPTLC, Pharmacognosy

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