Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method

Pharmacokinetics studies of domperidone generally analyze plasma matrix samples. The present work aimed to develop and validate a rapid and simple reversed phase-HPLC method for quantifying domperidone in plasma matrices. The chromatographic method implemented: 1. Luna Phenomenex® C18 (250 mm × 4.6 mm i.d; 5 µm) column, 2. isocratic mobile phase mixture of phosphate buffer 0.02 M:acetonitrile (70:30, v/v) with a flow rate of 1 mL/min, 3. UV detection at 285 nm. Domperidone and propranolol hydrochloride (as internal standard) were extracted from the deproteinated plasma sample. The method linearity was 0.998 in the range concentration of 15–200 ng/mL. The percentage of accuracy error was between -8.49–4.31%, while the percentage coefficient variation of precision ranged between 5.11–14.24%. This proposed method was simple, rapid (separation time less than 10 min), and selective. The validation parameters responses satisfied the method's requirements to determine domperidone in a plasma sample.

LUPEOL VALIDATION AND QUANTIFICATION IN HETEROPOGON CONTORTUS (L.) BEAUV. (SPEAR GRASS) THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

Objective: This is aimed to study the chromatographic evaluation of triterpenoid, i.e., lupeol from methanolic extracts of leaves, stem, and inflorescence of Heteropogon contortus.Methods: The high-performance thin-layer chromatography (HPTLC) densitometry determination of lupeol was performed using optimized mobile phase toluene:methanol:formic acid (7:3:0.3 v/v) with a derivatization of freshly prepared anisaldehyde-sulfuric acid. For densitometry measurements, the plates were scanned at 530 nm absorbance/reflectance wavelength. Quantification of lupeol marker compound in H. contortus leaves, stem, and inflorescence is estimated using 2-12 μg/spot.Results: The appearance of light purple bands on the chromatograms confirmed the lupeol component in plant samples. Further, the confirmation of the compound is done from the densitometric scanning by comparing λmax values. From this, it is reported that lupeol is present in leaf samples, i.e., 10 mg/g of dry wt., while in rest of the two samples, it is found absent.Conclusion: The leaves of H. contortus (spear grass) are a good source of lupeol and can be used as an alternate natural source to synthesize herbal drugs to control cancer and other anti-inflammatory agents. The presently selected HPTLC is validated and most accurate for the quantification and identification of lupeol present in the selected plant. The leaves of the species which are rich in lupeol can be used in pharmaceutical industry.