Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

ABSTRACT Malaria is an infectious disease caused by the Plasmodium parasite (class of Sporozoa) that attacks red blood cells. In indonesia, there are 4 (four) species of malaria parasites, namely Plasmodium falciparum as the cause of tropical malaria which often causes brain malaria with death, Plasmodium vivax as the cause of malaria tertiana, Plasmodium malariae as the cause of malaria quartana and Plasmodium ovale as the cause of ovale malaria. This study aims to determine the patient characteristics and treatment patterns in malaria patients at the inpatient installation of the Mimika District Hospital in period June – December 2018. This study was a descriptive study with retrospective data collection against 68 medical records of malaria patients who were hospitalized inclusion criteria. There were more men group than women, 41 patiensts (60,29%). Most of malaria patients are in the 0 – 5 year age group with 30 patients (44,12%). The most common type of malaria is uncomplicated vivax malaria 21 patients (33,82%). The most drug use of patients was Primaquin as many as 57 (83,82%) of 68 patients. The exact percentage of drug use for patients is 100%, right drug 89.71%, right dose 92.65%, timely 97.06% and right route 100%. Keywords : Malaria, Drug Utilization Study, Hospitalization. ABSTRAK Malaria merupakan penyakit menular disebabkan oleh parasit Plasmodium (kelas Sporozoa) yang menyerang sel darah merah. Di Indonesia dikenal 4 (empat) macam spesies parasit malaria yaitu Plasmodium falciparum sebagai penyebab malaria tropika yang sering menyebabkan malaria otak dengan kematian, Plasmodium vivax sebagai penyebab malaria tertiana, Plasmodium malariae sebagai penyebab malaria quartana dan Plasmodium ovale sebagai penyebab malaria ovale. Penelitian ini bertujuan untuk mengetahui karakteristik pasien dan pola pengobatan penyakit malaria pada pasien malaria di instalasi rawat inap RSUD Kabupaten Mimika periode bulan Juni – Desember 2018. Penelitian ini merupakan penelitian deskriptif dengan pengambilan data secara retrospektif terhadap 68 data rekam medik pasien malaria rawat inap yang memenuhi kriteria inklusi. Jumlah pasien laki-laki lebih banyak dibandingkan perempuan yaitu sebanyak 41 pasien (60,29%). Pasien Malaria kebanyakan berada pada kelompok usia 0-5 tahun sebanyak 30 pasien (44,12%). Jenis malaria yang paling sering terjadi adalah Malaria vivax tanpa komplikasi sebanyak 23 pasien (33,82%). Penggunaan obat pasien paling banyak yaitu Primakuin sebanyak 57 (83,82%) dari total 68 pasien. Persentase ketepatan penggunaan obat yang tepat pasien 100%, tepat obat 89,71%, tepat dosis 92.65%, tepat waktu 97,06% dan tepat rute 100%. Kata kunci : Malaria, Studi Penggunaan Obat, Rawat Inap.

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Hacia un mundo libre de malaria: Utopía o realidad

Boletín de Malariología y Salud Ambiental ◽

10.52808/bmsa.7e5.613.001 ◽

2021 ◽

Vol 61 (3) ◽

pp. 373-382

Author(s):

Alberto Sánchez Garrido

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Plasmodium Knowlesi ◽

Plasmodium Malariae ◽

Plasmodium Ovale ◽

Plasmodium Ovale Curtisi ◽

America Latina

La Malaria es una enfermedad causada por un parásito que se transmite a los humanos a través de la picadura de mosquito hembra Anophele. Reportando la WHO en el 2019, 229 millones de casos y 409.000 muertes por la enfermedad en 87 paises del mundo, Existen seis especies de este párasito: Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale wallickeri, Plasmodium ovale curtisi, Plasmodium malariae y Plasmodium knowlesi. Siendo la especie P. falciparum la causante de mayor morbilidad, con tasa entre 10 y 50% de mortalidad por malaria complicada. Alrededor de 108 países han declarado la malaria como enfermedad endémica, pudiendo padecer la enfermedad en cualquier época del año. Sin embargo, en el caso de América Latina hoy en día se vive un estancamiento de la enfermedad, reportándose en países menos de 100 casos autóctonos entre el 2000 y 2019, con algunas excepciones. Esta situación de vulnerabilidad de países como Brasil, Colombia, la frontera Perú-Ecuador, Venezuela, se incrementan ante la presencia activa de la pandemia producto del Covid -19 aunado a restricciones económicas, incremento de la actividad minera, o políticas públicas que ponen en riesgo la sostenibilidad del programa de control de la enfermedad. Para el 2021 la OMS corrobora que existen 87 países con malaria a nivel mundial, de los cuales 24 de ellos habían interrumpido su transmisión autóctona por 3 años. Realidad que consolidad la propuesta tras la experiencia adquirida, que cualquiera que sea la situación epidemiológica de entrada, el trabajo hacia la erradicación de la malaria debe entenderse y atenderse como un proceso continuo donde los propios Estados deben desde su realidad y estrategias propias se articulen con el Plan Estratégico Técnico Mundial Contra la Malaria 2016-2030 propuesto por la OMS. Palabras

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PLASMODIUM DOMINAN DALAM NYAMUK ANOPHELES BETINA (Anopheles spp.) PADA BEBERAPA TEMPAT DI DISTRIK MANOKWARI BARAT

Jurnal Natural ◽

10.30862/jn.v14i1.13 ◽

2018 ◽

Vol 14 (1) ◽

pp. 29-36

Author(s):

Rina A. Mogea

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Research Result ◽

Plasmodium Species ◽

Plasmodium Malariae ◽

Anopheles Mosquito ◽

Plasmodium Ovale ◽

Aedes Mosquito ◽

Anopheles Farauti ◽

Male Mosquito

Malaria contagious by mosquito Anopheles Betina bringing protozoa parasite in its body (Plasmodium). Plasmodium there are four specieses that is Plasmodium vivax, Plasmodium ovale, Plasmodium malariae and Plasmodium falciparum, but often becomes pathogen that is Plasmodium vivax and Plasmodium falciparum. As for intention of this research is to identify Plasmodium which is dominant at female Anopheles mosquito (Anopheles spp.) and knows distribution pattern of female Anopheles mosquito (Anopheles spp.) in some places in Districts Manokwari Barat. Based on research result done to four locations that is area Amban, Wosi, Sanggeng and Kota is found [by] 1024 mosquito tails. From the amounts only 115 mosquito tails was mosquito Anopheles Betina while the other is mosquito Anopheles male, mosquito Culex and Aedes. Mosquito Anopheles Betina found consisted of 4 species that is Anopheles bancrofti, Anopheles kochi, Anopheles farauti and Anopheles koliensis. Mosquito Anopheles Betina which is dissected, obtained 2 the Plasmodium species in mosquito spit gland is Plasmodium vivax and Plasmodium falciparum, and from calculation by index dominant can be told that both types of this very dominant Plasmodium in Districts Manokwari Barat because its the dominant index > 5%.

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SKRINING PARASIT MALARIA DI WILAYAH KERJA PUSKESMAS BUMI WONOREJO NABIRE-PAPUA TAHUN 2018

(Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) ◽

10.37887/jimkesmas.v5i1.10615 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Lymbran Tina ◽

Fergiana Bagubau ◽

Fifi Nirmala

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Plasmodium Knowlesi ◽

Plasmodium Malariae ◽

Plasmodium Ovale

Abstrak Malaria adalah salah satu penyakit yang disebabkan oleh infeksi parasit yang dapat menyebabkankematian pada manusia. Penyakit infeksi ini disebabkan oleh parasit Plasmodium yang hidup dan berkembangbiak dalam sel darah merah manusia dan ditularkan melalui gigitan nyamuk Anopheles. Ada lima spesiesPlasmodium yang menyerang manusia yaitu : Plasmodium vivax, Plasmodium falciparum, Plasmodium malariae,Plasmodium ovale dan Plasmodium knowlesi. Penelitian ini merupakan jenis penelitian deskriptif denganrancangan penelitian crossectional atau potong lintang. penelitian ini dilakukan pemeriksaan skrining denganmaksud untuk melihat positif atau negatif parasit malaria di dalam sampel darah mengunakan mikroskop.Skrining malaria merupakan suatu usaha untuk mencari dan menemukan penderita penyakit malaria yangtampak gejala klinis melalui suatu tes atau pemeriksaan, yang secara singkat dan sederhana sehingga dapatmemisahkan mereka yang sehat dari mereka yang kemungkinan besar menderita penyakit malaria, yangselanjutnya diproses melalui diagnosis dan pengobatan. Penelitian ini dilakukan di laboratorium puskesmas BumiWonorejo kabupaten Nabire provinsi Papua tahun 2018. Sampel dalam penelitian ini adalah sebanyak 30 orang. Yaitu orang dengan gejala klinis seperti demam periodik, nyeri pada persendian, mual dan tinggal di wilayah kerjapuskesmas Bumi Wonorejo Nabire-Papua serta subjek bersedia di ambil sampel darah. Berdasarkan gambaran hasil pemeriksaan mikroskopis di Puskesmas Bumi Wonorejo Nabire-Papua tahun 2018 pada sediaan darahsubjek penelitian di peroleh positif malaria sebanyak 19 orang ( 63,3%) dan negatif malaria yaitu 11 0rang(36,3%). Sehingga dapat disimpulkan bahwa kasus positif malaria di wilayah kerja Puskesmas Bumi WonorejoNabirePapuatahun2018masihtinggi. Katakunci:skrining,mikroskopis,malari

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Protozoal infection: Malaria

10.1093/med/9780199568741.003.0317 ◽

2018 ◽

Author(s):

Nick Beeching ◽

Lynsey Goodwin ◽

Joanna Peters

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Human Disease ◽

Plasmodium Knowlesi ◽

Plasmodium Malariae ◽

Plasmodium Ovale ◽

Protozoal Infection

Malaria is a devastating protozoal infection that affects more than 200 million people worldwide each year, killing up to a million. Five species of Plasmodium are recognized causes of human disease: Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, and Plasmodium knowlesi.

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Malaria serology data from the Guiana shield: first insight in IgG antibody responses to Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae antigens in Suriname

Malaria Journal ◽

10.1186/s12936-020-03434-y ◽

2020 ◽

Vol 19 (1) ◽

Author(s):

Mergiory Y. Labadie-Bracho ◽

Farah T. van Genderen ◽

Malti R. Adhin

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Reference Population ◽

Plasmodium Malariae ◽

Dried Blood Spots ◽

Igg Antibody ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Malaria Burden ◽

Seroprevalence Data

Abstract Background Suriname has accomplished a steep decline in malaria burden, even reaching elimination levels. Plasmodium serology data are not available for Suriname and even extremely scarce within the region, therefore malaria serology testing was introduced, country customized cut-off values were determined and a study was performed to explore the antibody status for Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae. Methods A cross-sectional survey was conducted between July 2017 and March 2018 in two areas of the interior with different malaria settings: Stoelmanseiland, representing Maroon villages and Benzdorp, a gold mining area, with mostly Brazilian miners. Dried blood spots (DBS) were collected (n = 197) and antibody presence against seven Plasmodium antigens was detected using a multiplex bead-based, IgG antibody assay. Demographic information was gathered through a questionnaire. Country customized cut-off values were generated from a Surinamese malaria-naïve reference population (n = 50). Results Serological analysis for the reference population revealed cut-off values ranging from 14 MFI for LSA-1 to 177 MFI for PmMSP-119. Seroprevalence against any of the three MSP-119 antibodies was similar in both regions and surpassed 75%. Single seropositivity against PfMSP-119 antibodies was higher in Stoelmanseiland (27.0%) than Benzdorp (9.3%), in line with the historical malaria burden of Stoelmanseiland, while the reverse was observed for PvMSP-119 antibodies. Despite sporadic reports of P. malariae infections, PmMSP-119 antibody presence was 39.6%. A more detailed examination of P. falciparum serology data displayed a higher seroprevalence in villagers (90.7%) than in Brazilians (64.6%) and a highly diverse antigenic response with 22 distinct antibody combinations. Conclusions The results on the malaria antibody signature of Maroon villagers and Brazilian miners living in Suriname displayed a high Plasmodium seroprevalence, especially for P. falciparum in villagers, still reflecting the historical malaria burden. The seroprevalence data for both regions and the observed combinations of P. falciparum antibodies provided a valuable dataset from a historically important region to the international malaria serology knowledge. First insight in malaria serology data for Suriname indicated that the use of other target groups and assessment of age-dependent seroprevalence are required to successfully use malaria serology as tool in the national elimination strategy.

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PO 8418 NEW MALARIA EPIDEMIOLOGY IN COASTAL LAGOON OF BENIN: PLASMODIUM INFECTION IN ANOPHELES MELAS

BMJ Global Health ◽

10.1136/bmjgh-2019-edc.90 ◽

2019 ◽

Vol 4 (Suppl 3) ◽

pp. A35.1-A35

Author(s):

Claudiane Adigbonon ◽

Benoît S Assogba ◽

Luc S Djogbenou

Keyword(s):

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Malaria Transmission ◽

Coastal Lagoon ◽

Plasmodium Malariae ◽

Dry Season ◽

Anopheles Coluzzii ◽

Plasmodium Ovale ◽

Malaria Epidemiology ◽

Tropical Areas

IntroductionMalaria is a worldwide disease affecting many people particularly in the tropical and sub-tropical areas. It is caused by Plasmodium parasites and essentially transmitted by female mosquitoes belonging to the Anopheles genus. Our understanding of the infectivity of these vectors to Plasmodium is necessary to design sustainable strategies for their control. This aspect remains unknown in the coastal and lagoon area of Benin where Anopheles melas and Anopheles coluzzii are sympatric. This study aims to investigate the infectivity of these two vectors to Plasmodium to understand their role in malaria transmission in southern Benin.MethodsInsecticide spray catch technique was used to collect females in 80 houses randomly selected in our study site. Three hundred and twenty (320) females were identified using PCR–species technique, Plasmodium infection was determined by the TaqMan method during the dry season. This assay detects all four malaria-causing species and discriminates Plasmodium falciparum from Plasmodium ovale, Plasmodium vivax and Plasmodium malariae (OVM).ResultsDuring the dry season, the sporozoïte rates were 0.2% and 0.3% for Anopheles melas and Anopheles coluzzii, respectively. However, we observed that positivity to the OVM (one of Plasmodium ovale, Plasmodium vivax and Plasmodium malariae species) was significantly higher in Anopheles melas (95%) than in Anopheles coluzzii (33.33%) (Chi-sq=15 857, df=1, p<0.001). These results indicated that Anopheles melas is more infected by one of the species Plasmodium ovale, Plamodium vivax and Plasmodium malariae than by Plasmodium falciparum, contrarily to Anopheles coluzzii.ConclusionThese findings reinforce the debate on the role of Anopheles melas in malaria transmission in coastal lagoon areas of Benin.

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"DIAGNOSA KEPERAWATAN PADA PASIEN PENYAKIT MALARIA"

10.31219/osf.io/vzua6 ◽

2020 ◽

Author(s):

Awina Milla Shilmy Sitorus

Keyword(s):

Plasmodium Falciparum ◽

World Health Organization ◽

Plasmodium Vivax ◽

Plasmodium Knowlesi ◽

Plasmodium Malariae ◽

World Health ◽

Plasmodium Ovale ◽

Health Organization

Diagnosis Keperawatan merupakan keputusan klinik tentang respon individu, keluarga dan masyarakat tentang masalah kesehatan aktual atau potensial, dimana berdasarkan pendidikan dan pengalamannya, perawat secara akuntabilitas dapat mengidentifikasi dan memberikan intervensi secara pasti untuk menjaga, menurunkan, membatasi, mencegah dan merubah status kesehatan klien (Yeni, 2008). Untuk dapat merumuskan diagnosa keperawatan dibutuhkan kemampuan analisis yang tinggi sehingga diperlukan sumber daya manusia yang capable dan mempunyai motivasi kuat untuk maju serta berpandangan maju (futuristic).Sampai saat ini malaria masih menjadi masalah kesehatan masyarakat Indonesia. Menurut World Health Organization (WHO), malaria adalah penyakit yang disebabkan oleh parasit plasmodium yang ditularkan kepada manusia melalui gigitan nyamuk Anopheles betina yang terinfeksi. Di Indonesia malaria pada manusia disebabkan oleh lima jenis spesies Plasmodium yaitu, Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae dan pada tahun 2012 ditemukan Plasmodium knowlesi khusus di Kalimantan Selatan. Penyakit ini tersebar luas di berbagai daerah, dengan derajat infeksi yang bervariasi. Pada bulan Juli-Agustus 2002, sejumlah daerah di Jawa Tengah dan Yogyakarta dilaporkan terserang wabah malaria. Di beberapa daerah yang telah belasan tahun tidak ada kasus malaria, tiba-tiba menjadi endemis kembali. Berdasarkan Survei Kesehatan Rumah Tangga (SKRT) 2012, di Indonesia setiap tahunnya terdapat sekitar 15 juta penderita malaria klinis yang mengakibatkan 30.000 orang meninggal dunia.

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