scholarly journals Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Chumbe ◽  
Ray Izquierdo-Lara ◽  
Katherine Calderón ◽  
Manolo Fernández-Díaz ◽  
Vikram N. Vakharia
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Neutralization Test ◽  
Green Fluorescent

Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein

Virus Genes ◽  
2017 ◽  
Vol 53 (3) ◽  
pp. 410-417 ◽  
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Satish Gaikwad ◽  
Dinesh C. Pathak ◽  
Vikram N. Vakharia
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Newcastle Disease Virus Strain ◽  
Green Fluorescent ◽  
Recombinant Newcastle Disease Virus

Characterization of a recombinant Newcastle disease virus expressing the green fluorescent protein

2003 ◽  
Vol 108 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Ines Engel-Herbert ◽  
Ortrud Werner ◽  
Jens P. Teifke ◽  
Teshome Mebatsion ◽  
Thomas C. Mettenleiter ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
Recombinant Newcastle Disease Virus

scholarly journals Expression of Transgenes from Newcastle Disease Virus with a Segmented Genome

2008 ◽  
Vol 82 (6) ◽  
pp. 2692-2698 ◽  
Author(s):  
Qinshan Gao ◽  
Man-Seong Park ◽  
Peter Palese
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Gene Encoding ◽  
Gene Therapy Vectors ◽  
Efficient Replication ◽  
Dsred Gene ◽  
Green Fluorescent ◽  
Segmented Genome

ABSTRACT Paramyxoviruses belong to the Paramyxoviridae family of the order Mononegavirales. They have a nonsegmented negative-stranded RNA genome and can cause a number of diseases in humans and animals. We generated a recombinant Newcastle disease virus (NDV) possessing a two-segmented genome. Each genomic segment is flanked by authentic NDV 3′ and 5′ noncoding termini allowing for efficient replication and transcription. A reporter gene encoding green fluorescent protein (GFP) was inserted into one segment, and a red fluorescent protein dsRed gene was inserted into the other segment in order to easily detect the replication and transcription of segments in infected cells. The rescued viruses grew well and were stable in embryonated chicken eggs over multiple passages. We were able to detect the expression of both reporter genes in the same cell infected with the virus possessing a segmented genome, and viral particles can contain either one or two types of RNA segments. We also rescued a two-segmented virus expressing GFP and the severe acute respiratory syndrome-associated coronavirus spike S protein, which is about 200 kDa. The chimeric virus extends the coding capacity of NDV by 30%, suggesting that the two-segmented NDV can be used for development of vaccines or gene therapy vectors carrying long and multiple transgenes.

scholarly journals Role of Untranslated Regions in Regulation of Gene Expression, Replication, and Pathogenicity of Newcastle Disease Virus Expressing Green Fluorescent Protein

2009 ◽  
Vol 84 (5) ◽  
pp. 2629-2634 ◽  
Author(s):  
Shin-Hee Kim ◽  
Siba K. Samal
Keyword(s):  
Gene Expression ◽  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Untranslated Regions ◽  
Foreign Gene ◽  
Gfp Expression ◽  
Green Fluorescent

ABSTRACT To gain insight into the role of untranslated regions (UTRs) in regulation of foreign gene expression, replication, and pathogenicity of Newcastle disease virus (NDV), a green fluorescent protein (GFP) gene flanked by 5′ and 3′ UTRs of each NDV gene was individually expressed by recombinant NDVs. UTRs of each gene modulated GFP expression positively or negatively. In particular, UTRs of the M and F genes enhanced levels of GFP expression at the junction of the P and M genes without altering replication of NDV, suggesting that UTRs could be used for enhanced expression of a foreign gene by NDV.

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