scholarly journals Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Fahimeh Shooraj ◽  
Bahman Mirzaei ◽  
Seyed Fazlollah Mousavi ◽  
Farzaneh Hosseini
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Genomic Analysis ◽  
Clonal Diversity ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Genomic Diversity ◽  
Biochemical Tests ◽  
Genetic Origin ◽  
Susceptibility Tests

Abstract Objectives Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence of H. influenzae carriage, to characterize antibiotic susceptibility, and to explore genetic diversity of H. influenzae isolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure. Results Out of 328 nasopharynx swabs, 73 strains were identified as H. influenzae. Among H. influenzae isolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn’t show any significant correlation with the clonality of strains.

scholarly journals Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children

2019 ◽  
Author(s):  
Fahimeh Shooraj ◽  
Bahman Mirzaei(Former Corresponding Author) ◽  
Seyed Fazlollah Mousavi(New Corresponding Author) ◽  
Farzaneh Hosseini
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Genomic Analysis ◽  
Clonal Diversity ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Genomic Diversity ◽  
Biochemical Tests ◽  
Genetic Origin ◽  
Susceptibility Tests

Abstract Objectives: Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence of H. influenzae carriage, to characterize antibiotic susceptibility, and to explore genetic diversity of H. influenzae isolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure. Results: Out of 328 nasopharynx swabs,73 strains were identified as H. influenzae. Among H. influenzae isolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn’t show any significant correlation with the clonality of strains.

scholarly journals Clonal diversity of Haemophilus Influenzae carriage isolated from under the age of 3 years children

2019 ◽  
Author(s):  
Fahimeh Shooraj ◽  
Bahman Mirzaei ◽  
Seyed Fazlollah Mousavi ◽  
Farzaneh Hosseini
Keyword(s):  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Genomic Analysis ◽  
Clonal Diversity ◽  
Invasive Disease ◽  
Genomic Diversity ◽  
Isolation Rate ◽  
Biochemical Tests ◽  
Lack Of Information ◽  
Susceptibility Tests

Abstract Objectives: Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. Due to lack of information about nasopharyngeal isolation rate, epidemiology, antimicrobial resistance and genetic diversity of H. influenzae isolate from carries were assigned as main goals. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure Results: Out of 328 nasopharynx swabs, 73 strains were identified as H. influenzae. Susceptibility testing showed that levofloxacin is the most effective antibiotic against and the least effect belonged to tetracycline. Resistance to chloramphenicol (42.2%) and ampicillin (43.3%) was also observed. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn’t show any significant correlation with the clonality of strains.

scholarly journals Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate

2015 ◽  
Vol 144 (3) ◽  
pp. 576-581 ◽  
Author(s):  
M. MOHAMMED ◽  
N. DELAPPE ◽  
J. O'CONNOR ◽  
P. McKEOWN ◽  
P. GARVEY ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Gel Electrophoresis ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Outbreak Strain ◽  
Salmonella Dublin ◽  
Source Of Infection ◽  
Human Salmonellosis

SUMMARYSalmonella enterica subsp. enterica serovar Dublin is an uncommon cause of human salmonellosis; however, a relatively high proportion of cases are associated with invasive disease. The serotype is associated with cattle. A geographically diffuse outbreak of S. Dublin involving nine patients occurred in Ireland in 2013. The source of infection was not identified. Typing of outbreak associated isolates by pulsed-field gel electrophoresis (PFGE) was of limited value because PFGE has limited discriminatory power for S. Dublin. Whole genome sequencing (WGS) showed conclusively that the isolates were closely related to each other, to an apparently unrelated isolate from 2011 and distinct from other isolates that were not readily distinguishable by PFGE.

scholarly journals Investigation of genetic diversity of Salmonella enterica strains isolated from patients by Pulsed Field Gel Electrophoresis

2011 ◽  
Vol 5 (S1) ◽  
Author(s):  
Mohsen Ghafari ◽  
Bita Bakhshi ◽  
Mohamad Reza Pour Shafi ◽  
Nour Amir Mozafari ◽  
Mona Salimi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Association of IS1016 with the hia Adhesin Gene and Biotypes V and I in Invasive Nontypeable Haemophilus influenzae

2008 ◽  
Vol 76 (11) ◽  
pp. 5221-5227 ◽  
Author(s):  
Sarah W. Satola ◽  
Brooke Napier ◽  
Monica M. Farley
Keyword(s):  
Genetic Diversity ◽  
Haemophilus Influenzae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Division I ◽  
Emerging Infections ◽  
Nontypeable Haemophilus Influenzae ◽  
Insertion Element ◽  
Pulsed Field ◽  
Slide Agglutination

ABSTRACT A subset of invasive nontypeable Haemophilus influenzae (NTHI) strains has evidence of IS1016, an insertion element associated with division I H. influenzae capsule serotypes. We examined IS1016-positive invasive NTHI isolates collected as part of Active Bacterial Core Surveillance within the Georgia Emerging Infections Program for the presence or absence of hmw1 and hmw2 (two related adhesin genes that are common in NTHI but absent in encapsulated H. influenzae) and hia (homologue of hsf, an encapsulated H. influenzae adhesin gene). Isolates were serotyped using slide agglutination, confirmed as NTHI strains using PCR capsule typing, and biotyped. Two hundred twenty-nine invasive NTHI isolates collected between August 1998 and December 2006 were screened for IS1016; 22/229 (9.6%) were positive. Nineteen of 201 previously identified IS1016-positive invasive NTHI isolates collected between January 1989 and July 1998 were also examined. Forty-one IS1016-positive and 56 randomly selected IS1016-negative invasive NTHI strains were examined. The hia adhesin was present in 39 of 41 (95%) IS1016-positive NTHI strains and 1 of 56 (1.8%) IS1016-negative NTHI strains tested; hmw (hmw1, hmw2, or both) was present in 50 of 56 (89%) IS1016-negative NTHI isolates but in only 5 of 41 (12%; all hmw2) IS1016-positive NTHI isolates. IS1016-positive NTHI strains were more often biotype V (P < 0.001) or biotype I (P = 0.04) than IS1016-negative NTHI strains, which were most often biotype II. Pulsed-field gel electrophoresis revealed the expected genetic diversity of NTHI with some clustering based on IS1016, hmw or hia, and biotypes. A significant association of IS1016 with biotypes V and I and the presence of hia adhesins was found among invasive NTHI. IS1016-positive NTHI strains may represent a unique subset of NTHI strains, with characteristics more closely resembling those of encapsulated H. influenzae.

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