scholarly journals Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Celosia Lukman ◽  
Christopher Yonathan ◽  
Stella Magdalena ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
High Efficiency ◽  
Multiplicity Of Infection ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Pathogenic Escherichia Coli ◽  
Family Based

Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.

scholarly journals Genome sequence and characterization of five bacteriophages infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2

2020 ◽  
Author(s):  
Aël Hardy ◽  
Vikas Sharma ◽  
Larissa Kever ◽  
Julia Frunzke
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Viral Diversity ◽  
Isolation And Characterization ◽  
Phage Evolution ◽  
Transmission Electron ◽  
Family Based

AbstractStreptomyces are well-known antibiotic producers, and are also characterized by a complex morphological differentiation. Streptomyces, like all bacteria, are confronted with the constant threat of phage predation, which in turn shapes bacterial evolution. However, despite significant sequencing efforts recently, relatively few phages infecting Streptomyces have been characterized compared to other genera. Here, we present the isolation and characterization of five novel Streptomyces phages. All five phages belong to the Siphoviridae family, based on their morphology as determined by transmission electron microscopy. Genome sequencing revealed that four of them were temperate phages, while one had a lytic lifestyle. Moreover, one of the newly sequenced phages shows very little homology to already described phages, highlighting the still largely untapped viral diversity. Altogether, this study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.

scholarly journals Genome Sequence and Characterization of Five Bacteriophages Infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1065
Author(s):  
Aël Hardy ◽  
Vikas Sharma ◽  
Larissa Kever ◽  
Julia Frunzke
Keyword(s):  
Electron Microscopy ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Viral Diversity ◽  
Isolation And Characterization ◽  
Phage Evolution ◽  
Transmission Electron ◽  
Family Based ◽  
Siphoviridae Family

Streptomyces are well-known antibiotic producers, also characterized by a complex morphological differentiation. Streptomyces, like all bacteria, are confronted with the constant threat of phage predation, which in turn shapes bacterial evolution. However, despite significant sequencing efforts recently, relatively few phages infecting Streptomyces have been characterized compared to other genera. Here, we present the isolation and characterization of five novel Streptomyces phages. All five phages belong to the Siphoviridae family, based on their morphology as determined by transmission electron microscopy. Genome sequencing and life style predictions suggested that four of them were temperate phages, while one had a lytic lifestyle. Moreover, one of the newly sequenced phages shows very little homology to already described phages, highlighting the still largely untapped viral diversity. Altogether, this study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.

Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

2019 ◽  
Vol 82 (1) ◽  
pp. 164-167 ◽  
Author(s):  
PATRICK KINDLE ◽  
MAGDALENA NÜESCH-INDERBINEN ◽  
NICOLE CERNELA ◽  
ROGER STEPHAN
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Pcr Amplification ◽  
The United States ◽  
The European Union ◽  
Conventional Pcr ◽  
E Coli ◽  
Isolation And Characterization

ABSTRACT Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour samples were analyzed. After enrichment, the samples were screened for stx1 and stx2 by the Assurance GDS MPX ID assay. STEC strains were isolated and serotyped by the E. coli SeroGenoTyping AS-1 kit. The determination of stx subtypes was performed with conventional PCR amplification. Screening for eae, aggR, elt, and estIa/Ib was performed by real-time PCR. Nine (12.9%) of the flour samples tested positive for stx by PCR. STEC was recovered from eight (88.9%) of the positive samples. Two isolates were STEC O11:H48 harboring stx1c/stx1d, two were O146:H28 containing stx2b, one was O103:H2 containing stx1a and eae, and three were O nontypeable: Ont:H12 (stx2a), Ont:H14 (stx2a/stx2g), and Ont:H31 (stx1c/stx1d). STEC O103 belongs to the “top five” serogroups of human pathogenic STEC in the European Union, and STEC O146 is frequently isolated from diseased humans in Switzerland. Our results show that flour may be contaminated with a variety of STEC serogroups. Consumption of raw or undercooked flour may constitute a risk for STEC infection.

scholarly journals Isolation and Characterization of Type 1 Fimbriae from a Chicken Pathogenic Escherichia coli Serotype O78.

1992 ◽  
Vol 54 (6) ◽  
pp. 1145-1149 ◽  
Author(s):  
Tsutomu SEKIZAKI ◽  
Shigeru MIYAZAKI ◽  
Hiroya ITO ◽  
Tamae ASAWA ◽  
Isao NONOMURA
Keyword(s):  
Escherichia Coli ◽  
Type 1 Fimbriae ◽  
Isolation And Characterization ◽  
Pathogenic Escherichia Coli

scholarly journals Isolation and Characterization of a Bacteriophage That Hosts on Avian-pathogenic Escherichia coli (APEC)

2019 ◽  
Vol 18 (5) ◽  
pp. 223-230
Author(s):  
Haya Fakhouri ◽  
Orjowan Juneidi ◽  
Hana Al Mohtaseb ◽  
Murad Ishnaiwer ◽  
Fawzi Al- Razem
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Isolation And Characterization ◽  
Pathogenic Escherichia Coli

scholarly journals Isolation and Characterization of a New T-Even Bacteriophage, CEV1, and Determination of Its Potential To Reduce Escherichia coli O157:H7 Levels in Sheep

2006 ◽  
Vol 72 (9) ◽  
pp. 6405-6410 ◽  
Author(s):  
Raul R. Raya ◽  
Peter Varey ◽  
Rebecca A. Oot ◽  
Michael R. Dyen ◽  
Todd R. Callaway ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Oral Dose ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Isolation And Characterization ◽  
Unit Reduction

ABSTRACT Bacteriophage CEV1 was isolated from sheep resistant to Escherichia coli O157:H7 colonization. In vitro, CEV1 efficiently infected E. coli O157:H7 grown both aerobically and anaerobically. In vivo, sheep receiving a single oral dose of CEV1 showed a 2-log-unit reduction in intestinal E. coli O157:H7 levels within 2 days compared to levels in the controls.

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