Evaluation of the effect of probiotic as add-on therapy with conventional therapy and alone in malaria induced mice

Abstract Objective Chloroquine is used as a conventional drug therapy for the treatment of malaria. The existence of resistance to chloroquine shown among various species of Plasmodium leads to the search for more efficacious therapy to treat malaria. Probiotic (Lactobacillus casei) has been tried as an add-on therapy with chloroquine. Probiotics are ingested microorganisms associated with a beneficial effect on humans and other species. The study was done to check the efficacy of L. casei as an add-on therapy along with conventional drug therapy (chloroquine) to treat malaria. Results Probiotic in combination with chloroquine showed complete suppression in parasitemia rate. Representation of parasitemia rate was done using mean ± SD. p < 0.05 is considered as statistically significant. The results showed a reduction in parasitemia with probiotic treatment, which was further confirmed through histological observation of two major organs, the liver and spleen. Interestingly, further suppression of parasitemia and hemosiderosis was observed when probiotic was given along with chloroquine.

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Evaluation of Effect of Probiotics as an Add-on Therapy With Conventional Therapy and Alone in Malaria Induced Mice

10.21203/rs.3.rs-322290/v1 ◽

2021 ◽

Author(s):

Eshani Mahajan ◽

Shweta Sinha ◽

Alka Bhatia ◽

Rakesh Sehgal ◽

Bikash Medhi

Keyword(s):

Drug Therapy ◽

Beneficial Effect ◽

Conventional Therapy ◽

Lactobacillus Casei ◽

Complete Suppression ◽

Histological Observation ◽

Conventional Drug

Abstract Objective: Chloroquine is used as conventional drug therapy for the treatment of malaria. The existence of resistance for chloroquine shown among various species of Plasmodium leads to the search for more efficacious therapy to treat malaria. Probiotics (Lactobacillus casei) have been tried as add-on therapy with chloroquine. Probiotics are ingested microorganisms associated with a beneficial effect on humans and other species. The study was done to check the efficacy of probiotics as an add-on therapy along with conventional drug therapy (chloroquine) to treat malaria.Results: Probiotics in combination with chloroquine showed complete suppression in parasitemia count. Representation of parasitemia count was done using mean ± SD. P<0.05 is considered significant. The results showed a reduction in parasitemia with probiotics treatment, which was further confirmed through histological observation of two major organs liver and spleen. Interestingly, further suppression of parasitemia and hemosiderosis was observed when probiotics were given along with chloroquine.

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Probiotics in gastroenterology – from a different angle

Orvosi Hetilap ◽

10.1556/oh.2013.29547 ◽

2013 ◽

Vol 154 (8) ◽

pp. 294-304 ◽

Cited By ~ 2

Author(s):

György Miklós Buzás

Keyword(s):

Side Effects ◽

Beneficial Effect ◽

Analytical Data ◽

Well Being ◽

Gastrointestinal Diseases ◽

Convincing Evidence ◽

Probiotic Treatment ◽

History Of ◽

Irritable Bowel

After a short overview of the history of probiotics, the author presents the development of human intestinal microflora based on the newest genetic data and the microbiological features of main probiotics. The indications of probiotic administration have been defined and extended in recent years. The author reviews significant results of probiotic treatment in some gastrointestinal diseases based on meta-analytical data. Probiotics are useful in preventing and treating diarrhoea caused by antibiotics and Clostridium difficile caused diarrhoea. In the treatment of Helicobacter pylori infection, preparations containing certain Lactobacillus,Bifidobacterium strains or Saccaromyces boulardii could enhance by 5–10% the rate of successful eradication and reduce the incidence and severity of the side effects. Some symptoms of irritable bowel syndrome and thus the quality of life can be improved by probiotics. Their beneficial effect in ulcerative colitis was proven, while in Crohn’s disease has not yet been defined. The use of probiotics is not included in guidelines, with the exception of the Maastricht IV/Florence consensus. For each disease it is advisable to use probiotics containing strains only with proven beneficial effect. The efficiency of preparations containing mixed strains has not yet been properly investigated. The author reviews the rare but potentially serious side effects of probiotics. In Hungary, there are many probiotic preparations available which can be purchased in pharmacies without prescription: their use is more empirical than evidence-based. The European Food Safety Authority has recently rejected claims for probiotics to be classed as medicines given the lack of convincing evidence on the effects of probiotics on human health and well-being. Clearly, further research is needed to collect evidence which could be incorporated into the international guidelines. Orv. Hetil., 2013, 154, 294–304.

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CONVENTIONAL DRUG THERAPY IN INFLAMMATORY BOWEL DISEASE

Gastroenterology Clinics of North America ◽

10.1016/s0889-8553(21)00208-9 ◽

1995 ◽

Vol 24 (3) ◽

pp. 509-521

Author(s):

Mark G. Griffin ◽

Philip B. Miner

Keyword(s):

Inflammatory Bowel Disease ◽

Drug Therapy ◽

Bowel Disease ◽

Conventional Drug ◽

Inflammatory Bowel

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BAYESIAN META-ANALYSIS ON MEDICAL DEVICES: APPLICATION TO IMPLANTABLE CARDIOVERTER DEFIBRILLATORS

International Journal of Technology Assessment in Health Care ◽

10.1017/s0266462312000037 ◽

2012 ◽

Vol 28 (2) ◽

pp. 115-124 ◽

Cited By ~ 4

Author(s):

Ji-Hee Youn ◽

Joanne Lord ◽

Karla Hemming ◽

Alan Girling ◽

Martin Buxton

Keyword(s):

Drug Therapy ◽

Expert Opinion ◽

Probability Distributions ◽

Meta Analysis ◽

Mixed Treatment Comparison ◽

Implantable Cardioverter Defibrillators ◽

Class Iii ◽

Treatment Comparison ◽

Relative Risks ◽

Conventional Drug

Objectives: The aim of this study is to describe and illustrate a method to obtain early estimates of the effectiveness of a new version of a medical device.Methods: In the absence of empirical data, expert opinion may be elicited on the expected difference between the conventional and modified devices. Bayesian Mixed Treatment Comparison (MTC) meta-analysis can then be used to combine this expert opinion with existing trial data on earlier versions of the device. We illustrate this approach for a new four-pole implantable cardioverter defibrillator (ICD) compared with conventional ICDs, Class III anti-arrhythmic drugs, and conventional drug therapy for the prevention of sudden cardiac death in high risk patients. Existing RCTs were identified from a published systematic review, and we elicited opinion on the difference between four-pole and conventional ICDs from experts recruited at a cardiology conference.Results: Twelve randomized controlled trials were identified. Seven experts provided valid probability distributions for the new ICDs compared with current devices. The MTC model resulted in estimated relative risks of mortality of 0.74 (0.60–0.89) (predictive relative risk [RR] = 0.77 [0.41–1.26]) and 0.83 (0.70–0.97) (predictive RR = 0.84 [0.55–1.22]) with the new ICD therapy compared to Class III anti-arrhythmic drug therapy and conventional drug therapy, respectively. These results showed negligible differences from the preliminary results for the existing ICDs.Conclusions: The proposed method incorporating expert opinion to adjust for a modification made to an existing device may play a useful role in assisting decision makers to make early informed judgments on the effectiveness of frequently modified healthcare technologies.

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Oral administration of Lactobacillus casei Shirota can ameliorate the adverse effect of an acute aflatoxin exposure in Sprague Dawley rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000513 ◽

2018 ◽

Vol 88 (3-4) ◽

pp. 199-208

Author(s):

Elham Nikbakht ◽

Rosita Jamaluddin ◽

S. Mohd Redzwan ◽

Saman Khalesi

Keyword(s):

Adverse Effect ◽

Lactobacillus Casei ◽

Health Effect ◽

Acute Exposure ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Lactobacillus Casei Shirota ◽

Probiotic Treatment ◽

Significant Difference ◽

Aflatoxin B

Abstract. Aflatoxin B1 (AFB1) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Lactobacillus casei Shirota strain (LcS) induction in an acute exposure to AFB1 in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB1 only (n = 9); AFB1 treated with LcS (n = 9); and control (no AFB1 exposure) (n = 6) groups. The blood AFB1 level of rats treated with LcS was slightly lower than the untreated AFB1 induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g–1). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB1 induced rats. However, a trend for a significant difference was only observed in ALT of AFB1 induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, p = 0.06). Rats’ body weight decreased in all animals force-fed with AFB1 with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB1 in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.

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The effect of probiotic treatment on elderly patients with distal radius fracture: a prospective double-blind, placebo-controlled randomised clinical trial

Beneficial Microbes ◽

10.3920/bm2016.0067 ◽

2016 ◽

Vol 7 (5) ◽

pp. 631-637 ◽

Cited By ~ 19

Author(s):

M. Lei ◽

L-M. Hua ◽

D-W. Wang

Keyword(s):

Clinical Trial ◽

Elderly Patients ◽

Distal Radius Fracture ◽

Distal Radius ◽

Lactobacillus Casei ◽

Radius Fracture ◽

Double Blind ◽

Double Blind Placebo ◽

Lactobacillus Casei Shirota ◽

Probiotic Treatment

Probiotic treatment has been shown to improve bone formation, increase bone mass density and prevent bone loss. We aimed to assess the effect of probiotic treatment on functional recovery in elderly patients with a distal radius fracture. A total of 417 elderly patients with an acute distal radius fracture were enrolled in this double-blind placebo-controlled clinical trial. They were randomised to receive skimmed milk containing either a commercial probiotic (Lactobacillus casei Shirota) or placebo daily for a period of 6 months after the fracture. Treatment outcomes were the DASH (disabilities of the arm, shoulder and hand) score, pain, complex regional pain syndrome (CRPS) score, active range of motion and grip strength, all of which were measured on a monthly basis. Throughout the duration of the study, DASH score, pain, CRPS score, wrist flexion and grip strength of patients receiving probiotics exhibited a significantly faster pace of improvement than those on placebo, with treatment outcomes of patients receiving Lactobacillus casei Shirota at month 4 at comparable levels with those of patients receiving placebo at month 6. In elderly patients with a fracture of the distal radius, administration of the probiotic could greatly accelerating the healing process.

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Chronotherapy versus conventional drug therapy for hypertension

10.1002/14651858.cd004184 ◽

2003 ◽

Cited By ~ 3

Author(s):

Ping Zhao ◽

P Xu ◽

Chao Min Wan ◽

B Y Li ◽

ZR Wang

Keyword(s):

Drug Therapy ◽

Conventional Drug

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Acupuncture for Pain Management in Cancer: A Systematic Review and Meta-Analysis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/1720239 ◽

2016 ◽

Vol 2016 ◽

pp. 1-13 ◽

Cited By ~ 24

Author(s):

Caiqiong Hu ◽

Haibo Zhang ◽

Wanyin Wu ◽

Weiqing Yu ◽

Yong Li ◽

...

Keyword(s):

Systematic Review ◽

Drug Therapy ◽

Remission Rate ◽

Data Extraction ◽

Meta Analysis ◽

Onset Time ◽

Risk Of Bias ◽

Conventional Drug ◽

Larger Sample

Objective. To evaluate the effectiveness and safety of acupuncture for cancer-related pain. Methods. A systematic review of literatures published from database inception to February 2015 was conducted in eight databases. RCTs involving acupuncture for treatment of cancer-related pain were identified. Two researchers independently performed article selection, data extraction, and quality assessment of data. Results. 1,639 participants in twenty RCTs were analyzed. All selected RCTs were associated with high risk of bias. Meta-analysis indicated that acupuncture alone did not have superior pain-relieving effects as compared with conventional drug therapy. However, as compared with the drug therapy alone, acupuncture plus drug therapy resulted in increased pain remission rate, shorter onset time of pain relief, longer pain-free duration, and better quality of life without serious adverse effects. However, GRADE analysis revealed that the quality of all outcomes about acupuncture plus drug therapy was very low. Conclusions. Acupuncture plus drug therapy is more effective than conventional drug therapy alone for cancer-related pain. However, multicenter high-quality RCTs with larger sample sizes are needed to provide stronger evidence for the effectiveness of acupuncture in cancer-related pain due to the low data quality of the studies included in the current meta-analysis.

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Treatment of pyoderma with radiant energy of artificial light sources

Kazan medical journal ◽

10.17816/kazmj63914 ◽

1937 ◽

Vol 33 (3) ◽

pp. 291-299

Author(s):

G. I. Kozmodemyansky

Keyword(s):

Drug Therapy ◽

Treatment Time ◽

Radiant Energy ◽

Artificial Light ◽

Light Sources ◽

Weak Effect ◽

Public Funds ◽

Conventional Drug ◽

Aniline Dyes ◽

Water Alcohol

Pyoderma diseases often lead to disability. The use of the most effective methods, which shorten the time of treatment of pyoderma diseases, will improve the health of workers, save many working days and give great savings in public funds. In recent years, the KSMI skin clinic has been successfully using topically solutions (water, alcohol) of aniline dyes and manganese peroxide for pyoderma. Of the negative aspects of this treatment, it should be noted that aniline dyes stain linen and have a weak effect in forms of deep dermal pyoderma (furuncle, carbuncle, hydradenitis). The simultaneous use of phototherapy with aniline dyes and manganese peroxide, as observations have shown, did not give us special advantages over conventional drug therapy in terms of shortening the treatment time.

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