scholarly journals Plasma amyloid-β oligomerization assay as a pre-screening test for amyloid status

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Rosha Babapour Mofrad ◽  
Philip Scheltens ◽  
SangYun Kim ◽  
Sungmin Kang ◽  
Young Chul Youn ◽  
...  
Keyword(s):  
Medical Information ◽  
Pearson Correlation ◽  
Amyloid Β ◽  
Amyloid Pet ◽  
Operating Characteristics ◽  
Storage Duration ◽  
Long Term Storage ◽  
Storage Effects ◽  
Term Storage

Abstract Objective We assessed the performance of plasma amyloid oligomerization tendency (OAβ) as a marker for abnormal amyloid status. Additionally, we examined long-term storage effects on plasma OAβ. Methods We included 399 subjects regardless of clinical diagnosis from the Amsterdam Dementia Cohort and European Medical Information Framework for AD project (age, 63.8 ± 6.6; 44% female). Amyloid status was determined by visual read on positron emission tomography (PET; nabnormal = 206). Plasma OAβ was measured using the multimer detection system (MDS). Long-term storage effects on MDS-OAβ were assessed using general linear models. Associations between plasma MDS-OAβ and Aβ-PET status were assessed using logistic regression and receiver operating characteristics analyses. Correlations between plasma MDS-OAβ and CSF biomarker levels were evaluated using Pearson correlation analyses. Results MDS-OAβ was higher in individuals with abnormal amyloid, and it identified abnormal Aβ-PET with an area under the curve (AUC) of 0.74 (95% CI, 0.67–0.81), especially in samples with a storage duration < 4 years. Combining APOEe4 and age with plasma MDS-OAβ revealed an AUC of 81% for abnormal amyloid PET status (95% CI, 74–87%). Plasma MDS-OAβ correlated negatively with MMSE (r = − 0.29, p < .01) and CSF Aβ42 (r = − 0.20, p < 0.05) and positively with CSF Tau (r = 0.20, p = 0.01). Conclusions Plasma MDS-OAβ combined with APOEe4 and age accurately identifies brain amyloidosis in a large Aβ-confirmed population. Using plasma MDS-OAβ as a screener reduced the costs and number of PET scans needed to screen for amyloidosis, which is relevant for clinical trials. Additionally, plasma MDS-OAβ levels appeared affected by long-term storage duration, which could be of interest for others measuring plasma Aβ biomarkers.

scholarly journals Plasma Amyloid-β Oligomerization Assay as a Pre-Screening Test for Amyloid Status

2020 ◽  
Author(s):  
Rosha Babapour Mofrad ◽  
Philip Scheltens ◽  
SangYun Kim ◽  
Sungmin Kang ◽  
Young Chul Youn ◽  
...  
Keyword(s):  
Medical Information ◽  
Pearson Correlation ◽  
Amyloid Β ◽  
Amyloid Pet ◽  
Operating Characteristics ◽  
Storage Duration ◽  
Long Term Storage ◽  
Storage Effects ◽  
Term Storage

Abstract Background: We assessed the performance of plasma amyloid oligomerization tendency (OAβ) as a marker for abnormal amyloid status. Additionally, we examined long-term storage effects on plasma OAβ. Methods: We included 399 subjects regardless of clinical diagnosis from the Amsterdam Dementia Cohort and European Medical Information Framework for AD project (age:63.8±6.6; 44% female). Amyloid status was determined by visual read on Positron Emission Tomography (PET; n abnormal= 206). Plasma OAβ was measured using the Multimer Detection System (MDS). Long-term storage effects on MDS-OAβ were assessed using General Linear Models. Associations between plasma MDS-OAβ and Aβ-PET status were assessed using logistic regression and receiver operating characteristics analyses. Correlations between plasma MDS-OAβ and CSF biomarker levels were evaluated using Pearson correlation analysesResults MDS-OAβ was higher in in individuals with abnormal amyloid, and it identified abnormal Aβ-PET with an Area Under the Curve (AUC) of 0.74 (95%CI: 0.67-0.81), especially in samples with short-term storage duration. Combining APOEe4 and age with plasma MDS-OAβ revealed an AUC of 81% for abnormal amyloid PET status (95%CI:74%-87%). Plasma MDS-OAβ correlated negatively with MMSE (r=-0.29,p<.01), and CSF Aβ42 (r=-0.20,p<0.05), and positively with CSF t-Tau (r=0.20,p=0.01).Conclusions: Plasma MDS-OAβ combined with APOEe4 and age, accurately identifies brain amyloidosis in a large Aβ-confirmed population. Using plasma MDS-OAβ as a screener reduced the costs and number of PET scans needed to screen for amyloidosis, which is relevant for clinical trials. Additionally, plasma MDS-OAβ levels appeared affected by long-term storage duration, which could be of interest for others measuring plasma Aβ biomarkers.

Air-drying and long-term storage effects on β-glucosidase, acid phosphatase and arylsulfatase activities in a tropical Savannah Oxisol

2015 ◽  
Vol 93 ◽  
pp. 68-77 ◽  
Author(s):  
André Alves de Castro Lopes ◽  
Djalma Martinhão Gomes de Sousa ◽  
Fábio Bueno dos Reis Junior ◽  
Ieda Carvalho Mendes
Keyword(s):  
Acid Phosphatase ◽  
Air Drying ◽  
Long Term Storage ◽  
Storage Effects ◽  
Term Storage

Changes in the content of organic acids and inorganic anions in sugar beet during long-term storage

2016 ◽  
pp. 760-764 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Paulina Miko ◽  
Magdalena Molska ◽  
Ilona Baszczyk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Storage Conditions ◽  
Denitrifying Bacteria ◽  
Raw Material ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Acetic Acids

Due to the prolongation of the period of the sugar campaign, it is necessary to optimize the storage conditions, so that changes in the quality of the raw material could be minimized. The aim of this study was to determine the effect of storage duration and temperature on changes in the composition of sugar beet. The study presents the changes in the content of glucose, fructose, raffinose, lactic and acetic acids, nitrates and nitrites as well as in the content of the total number of mesophilic bacteria, denitrifying bacteria and spores of denitrifying bacteria during storage under various conditions.

Storage duration increases oxidation products in peanut seeds

2021 ◽  
pp. 1-3
Author(s):  
Brandon Tonnis ◽  
Ming Li Wang ◽  
Edward Huang ◽  
Uttam Bhattarai ◽  
Shyam Tallury
Keyword(s):  
Oxidation Products ◽  
Oil Composition ◽  
Storage Duration ◽  
Plant Materials ◽  
Long Term Storage ◽  
Time Out ◽  
Total Oil ◽  
Changes Over Time ◽  
Term Storage

Abstract Seeds kept in long-term storage are essential for maintaining genetic resources of crops and other plant materials in seed banks and national germplasm systems. But seeds undergo chemical changes over time as part of the ageing process that result in reduced germination rates and seedling normalcy. For example, oilseed crops such as peanuts are particularly vulnerable to oxidation and rancidification due to their high oil content. To test the effect of storage time on seed oil in peanuts, we grew different accessions and harvested fresh seeds to compare the oil composition of new seeds with seeds aged in storage for varying lengths of time. Out of the nine fatty acids detected and measured by gas chromatography, five including oleic, gadoleic, behenic, lignoceric and cerotic acids differed significantly between new and old seeds. Additionally, old seeds accumulated up to four oxidation products together averaging about 1% of the total oil composition. The concentration of these oxidation products was positively correlated with the age of the seeds, increasing linearly up to more than 6% of the total in the oldest seeds. The presence and concentration of oxidation products measured using simple chromatography techniques can be used as an initial indication of quality and viability in older seed inventories stored in germplasm repositories.

Diapause induction and termination in Indian strains of Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

2017 ◽  
Vol 149 (5) ◽  
pp. 607-615 ◽  
Author(s):  
Enakshi Ghosh ◽  
Chandish R. Ballal
Keyword(s):  
Large Scale ◽  
Biocontrol Agent ◽  
Storage Temperature ◽  
Adult Emergence ◽  
Diapause Induction ◽  
Storage Duration ◽  
Trichogramma Chilonis ◽  
Long Term Storage ◽  
Term Storage

AbstractThe role of temperature in diapause induction was studied as a mode of long-term storage of Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae). Three different strains of this widely used biocontrol agent (T. chilonis Nilgiris strain, T. chilonis Kodaikanal strain, and T. chilonis 15 °C strain) reared on the factitious host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) were used for this experiment. Except T. chilonis laboratory strain, all the other strains could successfully undergo diapause at their pre-pupal stage. Maximum percentage of healthy pre-pupae were recorded in the three strains by providing a pre-storage temperature of 10 °C for 35 days with eight hours of photophase wherein 75–87% could enter into diapause. Further, at a maintenance temperature of 5 °C with 24 hours of scotophase, diapause could be maintained. Diapause could be terminated after six months of storage with 23–36% of adult emergence. However, there was significant reduction in longevity and parasitism rate of the emerged adults. Considering superior biological parameters, 95 days of storage (including pre-storage duration) could provide around 60% adult emergence. Successful long-term storage of T. chilonis strains through diapause induction can facilitate commercial insectaries in stockpiling this biocontrol agent for large-scale field releases. This is the first study on successful induction and termination of diapause in T. chilonis strains and evaluating their performance attributes.

scholarly journals Influence of Frozen Storage Duration and Forcing Temperature on Flowering of Oriental Hybrid Lilies

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1053-1056 ◽  
Author(s):  
Jong Suk Lee ◽  
Mark S. Roh
Keyword(s):  
Air Conditioning ◽  
Floral Development ◽  
Frozen Storage ◽  
High Quality ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Oriental Hybrid Lilies ◽  
Scanning Electron

The effect of long-term storage of lily bulbs at -2 °C (frozen storage) and of high forcing temperatures on plant height and floral abnormalities was investigated with Oriental hybrid lilies from 1998 to 2000. `Acapulco' and `Simplon' bulbs were stored frozen at -2 °C for various lengths of time and were forced in fan- and pad-cooled greenhouses with temperatures ranging from 11 to 31 °C, depending on the season. The same cultivars were also forced in greenhouses and maintained year-round under refrigerated air conditioning with day/night temperatures of 16/15.5 °C or 18.5/18 °C. Floral development immediately after storage and at different intervals thereafter was observed by scanning electron microscopy (SEM). The prolonged frozen storage reduced the number of flowers. High greenhouse forcing temperatures during summer significantly accelerated flowering, resulted in short plants, and increased the number of abnormal flowers. Forcing at a low temperature (15.5 °C) after planting the frozen stored bulbs resulted in longer cut stems than those forced at 25 °C for 30 days after potting. Bulbs can be stored up to 9 months and still produce high-quality Oriental hybrid lilies.

scholarly journals Response of Broccoli Transplants to LED Light during Short and Long-Term Storage

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1009
Author(s):  
Renata Wojciechowska ◽  
Edward Kunicki ◽  
Olga Długosz-Grochowska ◽  
Anna Kołton
Keyword(s):  
Light Treatment ◽  
Physiological Parameters ◽  
Storage Duration ◽  
Led Light ◽  
Light Spectra ◽  
Remote Locations ◽  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

In broccoli production a big challenge is preparation of seedlings, which must be delivered during a strictly defined period to vegetable producers that are often in distant places. The goal of the present study was to investigate the response of 5-week old broccoli transplants cv. Parthenon F1 to 2 and 6 weeks of storage at 4 °C under two LED light spectra (L1: 29% red 660 nm + 14% red 630 nm + 42% blue 250 nm + 15% green 520 nm and L2: 38% red 660 nm + 18% red 630 nm +26% blue 450 nm + 15% green 520 nm + 3% UV-A 330 nm; photoperiod 16/8 h day/night and PPFD 30 ± 10 µmol m−2 s−1) and darkness. Control 5-week-old seedlings were not stored. Light treatment improved the morphology and physiological parameters of seedlings. The yield of broccoli from control seedlings was similar to that obtained after L2 treatment, regardless of seedling storage duration. The most promising effects were obtained by adding 3% UV-A radiation to the lighting spectrum. The results are applicable to the transport of broccoli transplants to remote locations or storage for several weeks, in cases where it is necessary to delay planting.

Sign in / Sign up

Export Citation Format

Share Document