scholarly journals Influence of Frozen Storage Duration and Forcing Temperature on Flowering of Oriental Hybrid Lilies

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1053-1056 ◽  
Author(s):  
Jong Suk Lee ◽  
Mark S. Roh
Keyword(s):  
Air Conditioning ◽  
Floral Development ◽  
Frozen Storage ◽  
High Quality ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Oriental Hybrid Lilies ◽  
Scanning Electron

The effect of long-term storage of lily bulbs at -2 °C (frozen storage) and of high forcing temperatures on plant height and floral abnormalities was investigated with Oriental hybrid lilies from 1998 to 2000. `Acapulco' and `Simplon' bulbs were stored frozen at -2 °C for various lengths of time and were forced in fan- and pad-cooled greenhouses with temperatures ranging from 11 to 31 °C, depending on the season. The same cultivars were also forced in greenhouses and maintained year-round under refrigerated air conditioning with day/night temperatures of 16/15.5 °C or 18.5/18 °C. Floral development immediately after storage and at different intervals thereafter was observed by scanning electron microscopy (SEM). The prolonged frozen storage reduced the number of flowers. High greenhouse forcing temperatures during summer significantly accelerated flowering, resulted in short plants, and increased the number of abnormal flowers. Forcing at a low temperature (15.5 °C) after planting the frozen stored bulbs resulted in longer cut stems than those forced at 25 °C for 30 days after potting. Bulbs can be stored up to 9 months and still produce high-quality Oriental hybrid lilies.

scholarly journals Unconventional effects of long-term storage of microwave-modified chicken egg white lysozyme preparations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grzegorz Leśnierowski ◽  
Tianyu Yang ◽  
Renata Cegielska-Radziejewska
Keyword(s):  
Microwave Radiation ◽  
Egg White ◽  
Process Time ◽  
High Quality ◽  
Chicken Egg ◽  
Egg White Lysozyme ◽  
Physicochemical Changes ◽  
Long Term Storage ◽  
Term Storage

AbstractThermal modification is an effective method that induces significant expansion of the antimicrobial properties and other valuable properties of chicken egg white lysozyme. In our latest research, a new innovative method of enzyme modification was developed, in which microwave radiation was used as an energy source to process liquid lysozyme concentrate (LLC). After modification, high-quality preparations were obtained. However, long-term storage in a concentrated form initiated various processes that caused darkening over time and could also lead to other significant changes to their structure and, consequently, to their functional properties. This necessitated multidirectional research to explain this phenomenon. This paper presents the results of research aimed at assessing the physicochemical changes in the properties of microwave-modified lysozyme in the form of a liquid concentrate after long-term storage under refrigeration conditions. The assessment also considered the conditions under the acidity of the modifying medium and the duration of the microwave modification. The analysis showed that the values of the basic parameters determining the quality and usefulness of the modified enzyme significantly improved during long-term storage of the preparations. The greatest changes were observed in the preparations modified for the longest time and in the most acidic environment (process time 260 s, pH 2.0), the number of oligomers under these conditions increased by 18% after 12 months of holding, and the surface hydrophobicity increased by as much as 31%. In addition, microbiological tests showed that the preparations of microwave-modified lysozyme had an effect on gram-positive bacteria as well as on gram-negative, and this effect was significantly enhanced after 12 months. The results confirm that LLC modification with microwave radiation is a highly efficient method to prepare high-quality and high utility potential lysozyme. Notably, an interesting and important phenomenon was the observation of the unconventional behaviour of the preparations during their long-term storage, which increased their utility potential significantly.

Summer Pruning: An Ecological Alternative to Postharvest Calcium Treatment to Improve Storability of High Quality Apple cv. ‘Reinette du Canada’

2010 ◽  
Vol 16 (4) ◽  
pp. 343-350 ◽  
Author(s):  
M. Guerra ◽  
P.A. Casquero
Keyword(s):  
Titratable Acidity ◽  
Soluble Solids ◽  
High Quality ◽  
Long Term Storage ◽  
Postharvest Application ◽  
Bitter Pit ◽  
Best Parameter ◽  
Term Storage ◽  
Organic Orchards

Two strategies, summer pruning and postharvest Ca treatment, were studied in apple (Malus domestica Borkh) cv. ‘Reinette du Canada’ in order to analyze its effect on the fruit quality during storage. Summer pruning and Ca treatment reduced external and internal bitter-pits; so after 180 days of storage, both treatments decreased external bitter-pit by 10.0% and 16.7%, respectively. Summer pruning influenced color, firmness, total soluble solids and titratable acidity (TA) of fruit during storage, whereas Ca treatment only affected firmness and TA. Fruit from pruned trees had significant lower K and Mg than those from unpruned trees and Ca treatment increased Ca content. Orchard management, by means of summer pruning, combined with Ca postharvest application would be useful to prevent losses due to bitter-pit during storage in commercial orchards. However, in organic orchards, summer pruning would be the ecological alternative to decrease bitter-pit incidence during storage in high quality apple cv. ‘Reinette du Canada’. K/Ca ratio, on the peel at harvest, turned out to be the best parameter to correlate with external and internal bitter-pits during storage; so this ratio would be useful to predict bitter-pit on long-term storage.

scholarly journals Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 93 ◽  
Author(s):  
Cora McHugh ◽  
Thomas Flott ◽  
Casey Schooff ◽  
Zyad Smiley ◽  
Michael Puskarich ◽  
...  
Keyword(s):  
Human Serum ◽  
1H Nmr ◽  
Whole Blood ◽  
Nmr Spectra ◽  
High Quality ◽  
Healthy Human ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

Understanding Actinides through the Role of 5f Electrons

MRS Bulletin ◽  
2001 ◽  
Vol 26 (9) ◽  
pp. 684-688 ◽  
Author(s):  
T. Gouder ◽  
F. Wastin ◽  
J. Rebizant ◽  
G.H. Lander
Keyword(s):  
Predictive Power ◽  
Quality Data ◽  
Nuclear Fuels ◽  
High Quality ◽  
High Quality Data ◽  
Long Term Storage ◽  
5F Electrons ◽  
Term Storage

Studies of the actinide elements and compounds were (and are) motivated by the need to characterize their structural and thermodynamic properties for the development of nuclear fuels and the treatment of waste, whether it be for long-term storage or ideas involving transmutation in high-powered accelerators. For the most part, tables giving these data exist, although the data for transuranium compounds are rather sparse. A much more difficult task is to understand the data and develop theories that have predictive power in this part of the periodic table. In doing this, however, we are confronted with the extremely complicated electronic structure of the 5f shell and the great paucity of high-quality data on materials containing transuranium isotopes.

Patterns of lichen photosynthesis and respiration following prolonged frozen storage

1978 ◽  
Vol 56 (17) ◽  
pp. 2119-2123 ◽  
Author(s):  
Douglas W. Larson
Keyword(s):  
Light Intensity ◽  
Low Temperatures ◽  
Physiological Activity ◽  
Frozen Storage ◽  
Experimental Systems ◽  
Long Term Storage ◽  
Photosynthesis And Respiration ◽  
Term Storage ◽  
Respiratory Responses

The effect of prolonged frozen storage on patterns of photosynthesis and respiration in the lichen Alectoria ochroleuca (Hoffm.) Massal. has been examined. The results indicate that this plant not only survives long-term exposure to low temperatures but also that its basic photosynthetic and respiratory responses to temperature, light intensity, and thallus moisture content are altered very little by long-term storage at −60 °C. This maintenance not only of absolute viability but also of the more subtle patterns of physiological activity suggests that such storage may be used to hold lichen material for use in multivariate experimental systems which require replicates having identical field pretreatment.

Changes in the content of organic acids and inorganic anions in sugar beet during long-term storage

2016 ◽  
pp. 760-764 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Paulina Miko ◽  
Magdalena Molska ◽  
Ilona Baszczyk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Storage Conditions ◽  
Denitrifying Bacteria ◽  
Raw Material ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Acetic Acids

Due to the prolongation of the period of the sugar campaign, it is necessary to optimize the storage conditions, so that changes in the quality of the raw material could be minimized. The aim of this study was to determine the effect of storage duration and temperature on changes in the composition of sugar beet. The study presents the changes in the content of glucose, fructose, raffinose, lactic and acetic acids, nitrates and nitrites as well as in the content of the total number of mesophilic bacteria, denitrifying bacteria and spores of denitrifying bacteria during storage under various conditions.

High-Definition Video Recording: Taking Sport Technology to the Operating Room

2018 ◽  
Vol 66 (08) ◽  
pp. 667-669 ◽  
Author(s):  
C. de Sierra ◽  
J. Cuenca-Castillo ◽  
F. Estevez-Cid
Keyword(s):  
Video Recording ◽  
Digital Recording ◽  
High Definition ◽  
High Quality ◽  
Video Sharing ◽  
Long Term Storage ◽  
Digital Contents ◽  
Economical System ◽  
Term Storage

AbstractFilming surgeries for teaching purposes, publications, and patient records has become increasingly popular as the systems for digital recording have evolved, becoming high-quality systems, both smaller and lighter. Digital recording allows long-term storage, retrieval, and database organization. In addition, sharing digital contents has also become easier since video sharing sites and social networks make it possible to upload these contents onto the Internet. We describe a simple and economical system for surgeons to record surgeries in high definition under sterile conditions without any interference with the surgeon's line of vision.

Storage duration increases oxidation products in peanut seeds

2021 ◽  
pp. 1-3
Author(s):  
Brandon Tonnis ◽  
Ming Li Wang ◽  
Edward Huang ◽  
Uttam Bhattarai ◽  
Shyam Tallury
Keyword(s):  
Oxidation Products ◽  
Oil Composition ◽  
Storage Duration ◽  
Plant Materials ◽  
Long Term Storage ◽  
Time Out ◽  
Total Oil ◽  
Changes Over Time ◽  
Term Storage

Abstract Seeds kept in long-term storage are essential for maintaining genetic resources of crops and other plant materials in seed banks and national germplasm systems. But seeds undergo chemical changes over time as part of the ageing process that result in reduced germination rates and seedling normalcy. For example, oilseed crops such as peanuts are particularly vulnerable to oxidation and rancidification due to their high oil content. To test the effect of storage time on seed oil in peanuts, we grew different accessions and harvested fresh seeds to compare the oil composition of new seeds with seeds aged in storage for varying lengths of time. Out of the nine fatty acids detected and measured by gas chromatography, five including oleic, gadoleic, behenic, lignoceric and cerotic acids differed significantly between new and old seeds. Additionally, old seeds accumulated up to four oxidation products together averaging about 1% of the total oil composition. The concentration of these oxidation products was positively correlated with the age of the seeds, increasing linearly up to more than 6% of the total in the oldest seeds. The presence and concentration of oxidation products measured using simple chromatography techniques can be used as an initial indication of quality and viability in older seed inventories stored in germplasm repositories.

scholarly journals FTA Cards for Preservation of Nucleic Acids for Molecular Assays: A Review on the Use of Cytologic/Tissue Samples

2018 ◽  
Vol 142 (3) ◽  
pp. 308-312 ◽  
Author(s):  
Gilda da Cunha Santos
Keyword(s):  
Nucleic Acids ◽  
Molecular Techniques ◽  
Long Distance ◽  
High Quality ◽  
Molecular Assays ◽  
Fta Cards ◽  
Long Term Storage ◽  
Term Storage

Context.— Traditional methods for storing histologic and cytologic specimens for future use in molecular assays have consisted of either snap-freezing with cryopreservation or formalin-fixing, paraffin-embedding the samples. Although snap-freezing with cryopreservation is recommended for better preservation of nucleic acids, the infrastructure and space required for archiving impose challenges for high-volume pathology laboratories. Cost-effective, long-term storage at room temperature; relatively easy shipment; and standardized handling can be achieved with formalin-fixed, paraffin-embedded samples, but formalin fixation induces fragmentation and chemical modification of nucleic acids. Advances in next-generation sequencing platforms, coupled with an increase in diagnostic, prognostic, and predictive molecular biomarkers have created a demand for high-quality nucleic acids. To address issues of the quality of nucleic acid and logistics in sample acquisition, alternatives for specimen preservation and long-term storage have been described and include novel universal tissue fixatives, stabilizers, and technologies. Objective.— To collect, retrieve, and review information from studies describing the use of nucleic acids recovered from cytologic/tissue specimens stored on Flinders Technology Associates (FTA, GE Whatman, Maidstone, Kent, United Kingdom) cards for downstream molecular applications. Data Sources.— An electronic literature search in the PubMed (National Center for Biotechnology Information, Bethesda, Maryland) database allowed the selection of manuscripts addressing the use of FTA cards for storage of cytologic samples for molecular analysis. Only articles published in English were retrieved. Conclusions.— The use of FTA cards is a versatile method for fostering multicenter, international collaborations and clinical trials that require centralized testing, long-distance shipment, and high-quality nucleic acids for molecular techniques. Studies with controlled temperature are required to test the quality of recovered RNA after long-term storage.

Diapause induction and termination in Indian strains of Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

2017 ◽  
Vol 149 (5) ◽  
pp. 607-615 ◽  
Author(s):  
Enakshi Ghosh ◽  
Chandish R. Ballal
Keyword(s):  
Large Scale ◽  
Biocontrol Agent ◽  
Storage Temperature ◽  
Adult Emergence ◽  
Diapause Induction ◽  
Storage Duration ◽  
Trichogramma Chilonis ◽  
Long Term Storage ◽  
Term Storage

AbstractThe role of temperature in diapause induction was studied as a mode of long-term storage of Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae). Three different strains of this widely used biocontrol agent (T. chilonis Nilgiris strain, T. chilonis Kodaikanal strain, and T. chilonis 15 °C strain) reared on the factitious host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) were used for this experiment. Except T. chilonis laboratory strain, all the other strains could successfully undergo diapause at their pre-pupal stage. Maximum percentage of healthy pre-pupae were recorded in the three strains by providing a pre-storage temperature of 10 °C for 35 days with eight hours of photophase wherein 75–87% could enter into diapause. Further, at a maintenance temperature of 5 °C with 24 hours of scotophase, diapause could be maintained. Diapause could be terminated after six months of storage with 23–36% of adult emergence. However, there was significant reduction in longevity and parasitism rate of the emerged adults. Considering superior biological parameters, 95 days of storage (including pre-storage duration) could provide around 60% adult emergence. Successful long-term storage of T. chilonis strains through diapause induction can facilitate commercial insectaries in stockpiling this biocontrol agent for large-scale field releases. This is the first study on successful induction and termination of diapause in T. chilonis strains and evaluating their performance attributes.

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