Regeneration of full-thickness skin defects by differentiated adipose-derived stem cells into fibroblast-like cells by fibroblast-conditioned medium

In recent years, the bioactive factors were utilized in exercise and athletic skin injuries. In this research, the concentrated conditioned medium of hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The conditioned medium of hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of protein in freeze-dried conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated hypoxia-preconditioned conditioned medium can reduce the wound size and accelerate the skin wound healing. The concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for wound care in clinical application.

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Adipose-Derived Stem Cells Improve Engraftment of Full-Thickness Skin Grafts by Increasing Angiogenesis

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2015.07.260 ◽

2015 ◽

Vol 221 (4) ◽

pp. S112

Author(s):

Michael S. Hu ◽

Wan Xing Hong ◽

Zeshaan N. Maan ◽

Min Hu ◽

Andrew S. Zimmermann ◽

...

Keyword(s):

Stem Cells ◽

Adipose Derived Stem Cells ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin

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The Effects of Stromal Vascular Fraction（SVF）Cells and Adipose-Derived Stem Cells (ADSCs) in Full-Thickness Skin Grafts

10.21203/rs.3.rs-633452/v1 ◽

2021 ◽

Author(s):

Zhou Feng ◽

Fei Miaomiao ◽

Gong Lunli ◽

Wang Guobao ◽

Zhang Jun ◽

...

Keyword(s):

Stem Cells ◽

Survival Rate ◽

Stromal Vascular Fraction ◽

Adipose Derived Stem Cells ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Masson Staining ◽

Better Than

Abstract Background: To investigate the role and difference of autologous stromal vascular fraction（SVF）cells and allogeneic adipose-derived stem cells(ADSCs) in full-thickness skin graft.Methods: SVF cells and ADSCs were prepared from the inguinal fat pad of the rats, and full-thickness skin on the back of the rats were transplanted in situ. then, SVF, ADSCs and PBS were injected under the graft. Gross survival, H-E staining, Masson staining, CD31 and VEGF immunofluorescence were observed on 3d, 7d and 14d respectively.Results: the survival rate of SVF group was the best, followed by ADSCs group (p<0.05). the H-E and Masson staining structures of the SVF group were better than those of the other two groups under the microscope. CD31 and VEGF expression in SVF group at 7 days and 14 days after surgery was more obvious than that in ADSCs group and PBS group.Conclusion: Subcutaneous injection of autologous SVF or allogeneic ADSCs cells can improve the survival rate of full-thickness skin grafts. In addition, the effect of autologous SVF cells is better than that of allogeneic ADSCs.

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Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

Stem Cells International ◽

10.1155/2016/8281235 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Wu Minjuan ◽

Xiong Jun ◽

Shao Shiyun ◽

Xu Sha ◽

Ni Haitao ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Hair Follicle ◽

Amniotic Membrane ◽

Functional Restoration ◽

Skin Wounds ◽

Full Thickness ◽

Skin Defects ◽

Full Thickness Skin ◽

Thickness Skin

Early repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated and cultured adipose derived mesenchymal stem cells (ADMSCs) onto the human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to the surface of AAM and grew well 24 h after seeding. Comparison of the wound healing rate at days 7, 14, and 28 after transplantation showed that ADMSCs seeded on AAM facilitated the healing of full-thickness skin wounds more effectively as compared with either hAM or AAM alone, indicating that ADMSCs participated in skin regeneration. More importantly, we noticed a phenomenon of hair follicle development during the process of skin repair. Composite ADMSCs and AAM not only promoted the healing of the mouse full-thickness defects but also facilitated generation of the appendages of the affected skin, thus promoting restoration of the skin function. Our results provide a new possible therapy idea for the treatment of skin wounds with respect to both anatomical regeneration and functional restoration.

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