scholarly journals Resistance of bacteria isolated from leachate to heavy metals and the removal of Hg by Pseudomonas aeruginosa strain FZ-2 at different salinity levels in a batch biosorption system

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Fauzul Imron ◽  
Setyo Budi Kurniawan ◽  
Siti Rozaimah Sheikh Abdullah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Pseudomonas Aeruginosa ◽  
Fresh Water ◽  
Landfill Leachate ◽  
Saline Water ◽  
Resistant Bacteria ◽  
Sanitary Landfill ◽  
Salinity Levels ◽  
Sanitary Landfill Leachate

AbstractLeachate is produced from sanitary landfills containing various pollutants, including heavy metals. This study aimed to determine the resistance of bacteria isolated from non-active sanitary landfill leachate to various heavy metals and the effect of salinity levels on the removal of Hg by the isolated bacterium. Four dominant bacteria from approximately 33 × 1017 colony-forming units per mL identified as Vibrio damsela, Pseudomonas aeruginosa, Pseudomonas stutzeri, and Pseudomonas fluorescens were isolated from non-active sanitary landfill leachate. Heavy metal resistance test was conducted for Hg, Cd, Pb, Mg, Zn, Fe, Mn, and Cu (0–20 mg L− 1). The removal of the most toxic heavy metals by the most resistant bacteria was also determined at different salinity levels, i.e., fresh water (0‰), marginal water (10‰), brackish water (20‰), and saline water (30‰). Results showed that the growth of these bacteria is promoted by Fe, Mn, and Cu, but inhibited by Hg, Cd, Pb, Mg, and Zn. The minimum inhibitory concentration (MIC) of all the bacteria in Fe, Mn, and Cu was > 20 mg L− 1. The MIC of V. damsela was 5 mg L− 1 for Hg and >  20 mg L− 1 for Cd, Pb, Mg, and Zn. For P. aeruginosa, MIC was > 20 mg L− 1 for Cd, Pb, Mg, and Zn and 10 mg L− 1 for Hg. Meanwhile, the MIC of P. stutzeri was > 20 mg L− 1 for Pb, Mg, and Zn and 5 mg L− 1 for Hg and Cd. The MIC of P. fluorescens for Hg, Pb, Mg, and Zn was 5, 5, 15, and 20 mg L− 1, respectively, and that for Cd was > 20 mg L− 1. From the MIC results, Hg is the most toxic heavy metal. In marginal water (10‰), P. aeruginosa FZ-2 removed up to 99.7% Hg compared with that in fresh water (0‰), where it removed only 54% for 72 h. Hence, P. aeruginosa FZ-2 is the most resistant to heavy metals, and saline condition exerts a positive effect on bacteria in removing Hg.

scholarly journals Application of Electrocoagulation with a New Steel-Swarf-Based Electrode for the Removal of Heavy Metals and Total Coliforms from Sanitary Landfill Leachate

2021 ◽  
Vol 11 (11) ◽  
pp. 5009
Author(s):  
Mayk Teles de Oliveira ◽  
Ieda Maria Sapateiro Torres ◽  
Humberto Ruggeri ◽  
Paulo Scalize ◽  
Antonio Albuquerque ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Landfill Leachate ◽  
Lower Cost ◽  
Sanitary Landfill ◽  
Leachate Treatment ◽  
Removal Of Heavy Metals ◽  
Waste Characteristics ◽  
Electrode Passivation ◽  
Steel Swarf ◽  
Sanitary Landfill Leachate

Sanitary landfill leachate (LL) composition varies according to climate variables variation, solid waste characteristics and composition, and landfill age. Leachate treatment is essentially carried out trough biological and physicochemical processes, which have showed variability in efficiency and appear a costly solution for the management authorities. Electrocoagulation (EC) seems a suitable solution for leachate treatment taking into account the characteristics of the liquor. One of the problems of EC is the electrode passivation, which affects the longevity of the process. One solution to this problem could be the replacement of the electrode by one made of recyclable material, which would make it possible to change it frequently and at a lower cost. The objective of the present work was to evaluate the removal of heavy metals (As, Ba, Cd, Cr, Cu, Fe, Pb, Mn, Ni, Se and Zn) and coliforms from a LL by EC using electrodes made from steel swarf (SfE) up to 8 h. Removal efficiencies of detected heavy metals were 51%(Cr), 59%(As), 71%(Cd), 72%(Zn), 92%(Ba), 95%(Ni) and >99%(Pb). The microbial load of coliforms in leachate was reduced from 10.76 × 104 CFU/mL (raw leachate) to less than 1 CFU/mL (after treatment with SfE) (i.e., approximately 100% reduction). The use of SfE in EC of LL is very effective in removing heavy metals and coliforms and can be used as alternative treatment solution for such effluents.

Scale-up and cost analysis of a photo-Fenton system for sanitary landfill leachate treatment

2016 ◽  
Vol 283 ◽  
pp. 76-88 ◽  
Author(s):  
Tânia F.C.V. Silva ◽  
Amélia Fonseca ◽  
Isabel Saraiva ◽  
Rui A.R. Boaventura ◽  
Vítor J.P. Vilar
Keyword(s):  
Cost Analysis ◽  
Landfill Leachate ◽  
Scale Up ◽  
Sanitary Landfill ◽  
Leachate Treatment ◽  
Fenton System ◽  
Sanitary Landfill Leachate

scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

Post-treatment of sanitary landfill leachate by coagulation–flocculation using chitosan as primary coagulant

2016 ◽  
Vol 74 (1) ◽  
pp. 246-255 ◽  
Author(s):  
Inara Oliveira do Carmo Nascimento ◽  
Ana Rosa Pinto Guedes ◽  
Louisa Wessels Perelo ◽  
Luciano Matos Queiroz
Keyword(s):  
Acute Toxicity ◽  
Removal Efficiency ◽  
Landfill Leachate ◽  
Post Treatment ◽  
Toxicity Tests ◽  
Process Conditions ◽  
Sanitary Landfill ◽  
Average Growth ◽  
Acute Toxicity Tests ◽  
Sanitary Landfill Leachate

Chitosan was chosen as an alternative primary coagulant in a complementary coagulation–flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter. In order to optimize the process conditions, central composite design and response surface methodology were applied. To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al2 (SO4)3.14 H2O) as coagulant. In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms. The analytic hierarchy process was employed for selecting the most appropriate coagulant. Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L−1 at pH 8.5. The acute toxicity tests showed that organisms were sensitive to all samples, mainly after coagulation–flocculation using chitosan. CE50 for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC50 for P. reticulata was 23% (v v−1). Multi-criteria analysis showed that alum was the most appropriate coagulant. Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.

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