scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

scholarly journals Study on Heavy Metal Resistant Bacteria in Guheswori Sewage Treatment Plant

Our Nature ◽  
2009 ◽  
Vol 6 (1) ◽  
pp. 52-57 ◽  
Author(s):  
A. Rajbanshi
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Resistant Bacteria ◽  
Heavy Metal Resistant ◽  
Staphylococcus Spp ◽  
Multiple Tolerance

Removal of heavy metals from wastewater needs advance chemical technology and is more expensive too. The cheaper alternative for this is the bioremediation using heavy metals resistant microorganisms. In this study, 10 heavy metal resistant bacteria were isolated from oxidation ditch of wastewater treatment plant of Bagmati Area Sewerage Project. These include chromium resistant Staphylococcus spp, Escherichia coli, Klebsiella spp; cadmium resistant Acinetobacter spp, Flavobacterium spp, Citrobacter spp; nickel resistant Staphylococcus spp, Bacillus spp; copper resistant Pseudomonas spp; and cobalt resistant Methylobacterium spp. All the isolates showed high resistance to heavy metals with Minimum Inhibitor Concentration (MIC) for heavy metals ranging from 150 mug/ml to 500 mug/ml. Six resistant isolates showed multiple tolerance to heavy metals. All the 10 isolates also showed antibiotic resistance of which 10% were resistant to single antibiotic and 90% were multi-antibiotic resistant. Heavy metal tolerance test showed maximum microbial tolerance to chromium and minimum tolerance to nickel in mixed liquor sample of oxidation ditch.Keywords: Heavy metal resistant bacteria, multiple tolerance, antibiotic resistance, Guheswori Sewage Treatment Plantdoi: 10.3126/on.v6i1.1655Our Nature (2008)6:52-57

scholarly journals Heavy metal (As, Cd, Cr, Hg, Pb) analysis and identification of heavy metal resistant bacteria in sediments from Manado Bay

2021 ◽  
Vol 926 (1) ◽  
pp. 012096
Author(s):  
B J Kepel ◽  
W Bodhi ◽  
Fatimawali ◽  
T E Tallei
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Average Concentration ◽  
Resistant Bacteria ◽  
Icp Oes ◽  
Sediment Samples ◽  
Heavy Metal Concentrations ◽  
The World ◽  
Heavy Metal Resistant ◽  
16Srrna Gene

Abstract Environmental pollution from heavy metals is becoming a growing concern due to the adverse effects it is causing throughout the world. This study aims to analyze heavy metal concentrations and identify heavy metal resistant bacteria in the bay of Manado. Sediment samples were collected from five bays in Manado. The concentrations of heavy metals As, Cd, Cr, Hg and Pb were analyzed using ICP-OES, and Hg using CV-AFS. Bacteria from the sediment were grown in nutrient broth media containing heavy metals As, Cd, Cr, Hg and Pb respectively. Microbiology and 16SrRNA gene analysis were used to identify the bacteria that grown on media containing varying concentrations of heavy metals. The results showed that the sediments from the five bays in Manado contained heavy metals with an average concentration of As <1mg/kg, Cd 1.8mg/kg), Cr 6.2mg/kg, Hg <0.07mg/kg). and Pb 11.2mg/kg. The results of microbiological and molecular analysis showed that 5 species of heavy metal resistant bacteria were Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus arlettae, Acinobacter sp., and Brevibacterium sp. The five bacteria found to be resistant to heavy metals can be used to detoxify As, Cd, Cr, Hg, and Pb.

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

scholarly journals Diversity and Distribution of Heavy Metal-Resistant Bacteria in Polluted Sediments of the Araça Bay, São Sebastião (SP), and the Relationship Between Heavy Metals and Organic Matter Concentrations

2016 ◽  
Vol 72 (3) ◽  
pp. 582-594 ◽  
Author(s):  
Bruna Del Busso Zampieri ◽  
Aline Bartelochi Pinto ◽  
Leonardo Schultz ◽  
Marcos Antonio de Oliveira ◽  
Ana Julia Fernandes Cardoso de Oliveira
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Organic Matter ◽  
Resistant Bacteria ◽  
Polluted Sediments ◽  
Heavy Metal Resistant ◽  
The Relationship

scholarly journals Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Zhang ◽  
Rongrong Zhu ◽  
Weilin Li ◽  
Junwei Ma ◽  
Hui Lin
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotic Resistance Pattern ◽  
Degrading Bacterium ◽  
Drug Resistant Bacteria ◽  
Behavioural Characteristics ◽  
Degrading Bacteria ◽  
Arthrobacter Nicotianae

AbstractAlthough many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic-degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC-16, which shows substantial biodegradation of oxytetracycline (OTC)/tetracycline. The results showed that this strain could be resistant to at least seven categories of 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC-16 contains one chromosome (3,643,989 bp) and two plasmids (plasmid1, 123,894 bp and plasmid2, 29,841 bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During OTC degradation by the strain OTC-16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene (tetV) in the chromosome were tracked using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only the plasmid-derived resistance genes were up-regulated in the presence of OTC. The presence of OTC increased the tolerance of strain OTC-16 to streptomycin sulphate. The findings of this study can help deepen our understanding of the behavioural characteristics of resistance genes and adaptive evolution of drug-resistant bacteria.

scholarly journals Antibiotic Resistance Pattern of Bacteria Isolated from Nosocomial Infection in Internal Surgery and Neurosurgery Intensive Care Unit (NICU) at a Tertiary Care Hospital in Tehran, Iran

2017 ◽  
Vol 14 (3) ◽  
pp. 1095-1102 ◽  
Author(s):  
Mohammad Javad Hosseini ◽  
Mohamad Kiarsi ◽  
Reza Golmohammadi ◽  
Reza Sadripour
Keyword(s):  
Antibiotic Resistance ◽  
Intensive Care ◽  
Intensive Care Units ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Care Hospital ◽  
Drug Resistant ◽  
Antibiotic Resistance Pattern

ABSTRACT: Today, many studies doneregarding the antibiotic resistance showthose patients in intensive care units (ICUs) are more likely to develop drug resistant pathogens.Pathogens are the major cause of nosocomial infections in intensive care units. Knowledge of antibiotic resistance of pathogens in the hospital is very important for treatment. The recent study aims at identifying drug resistant pathogens and antibiotic resistance pattern in the intensive care units of a tertiary care hospital in Tehran. In other words, the main purpose of the recent study is understanding the degree to which the resistant bacteria, which cause nosocomial infections is spreading in the ICUs of a tertiary care hospital and to determine the antibiotic- resistance pattern of these bacteria in the ICUs of that hospital. The recent research is a retrospective and cross-sectional study that has been conducted on the files of all patients who were hospitalized in ICUs, internal surgery, and neurosurgery sections of Baqiyatallah Hospital in Tehran in a five-year period (March 2009 to February 2015) and who were diagnosed with nosocomial infection caused by resistant bacteria. According to the results of this research, most of the separated bacteria were: Acinetobacterbaumannii 39.45%, Pseudomonas aeruginosa 20.69% and the most common location for infection was respiratory tract (67.11%). Also the most common pathogen was Acinetobacterbaumannii which is resistant to most antibiotics.

scholarly journals Antibiotic Resistance Pattern Among Staphylococcus aureus Isolated From Wound Cultures in Burn Patients: A Five-Year Study

2020 ◽  
Vol 7 (4) ◽  
pp. 104-108
Author(s):  
Bahram Askarpour ◽  
Alireza Sedaghat ◽  
Nazanin Hazrati ◽  
Ali Ahmadabadi ◽  
Masoud Youssefi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Burn Patients ◽  
Burn Wound ◽  
Antibiotic Resistant ◽  
Antibiotic Resistance Pattern ◽  
Study Results

Background: Burn remains a globally significant life-threatening problem, especially in developing countries, and infection is considered as a major complication among burn patients. The rate of antibiotic-resistant bacteria isolated from burn patients has demonstrated a significant increase. In this regard, this study aimed to determine the antibiotic resistance pattern in Staphylococcus aureus isolated from patients’ burn wound infections. Methods: All available wound cultures of burn patients admitted to the burn unit of Emam-Reza hospital/ Mashhad, northeast Iran from March 2012 to March 2017 were included in this retrospective study. Then, the resistance of isolated S. aureus strains against 25 different antibiotic disks was studied based on the aim of the study. Results: Overall, 1973 patients were admitted, out of whom 4758 swab samples were taken from them. Out of 3188 micro-organisms isolated from burn wound cultures, 185 (5.8%) cases were S. aureus. Based on the results, the highest susceptibility rates were related to vancomycin (98.8%), cefazolin (72%), ciprofloxacin (75%), and gentamicin (74.6%). Conclusions: In general, vancomycin, cefazolin, and ciprofloxacin appeared to be the most effective agents among all tested antibiotics for S. aureus. The extensive use of antibiotics in treating infections has resulted in the emergence of resistant strains. Routine microbiological surveillance and careful in vitro testing before antibiotic use may help in the prevention of the ever-increasing antibiotic-resistant pathogens in burn infections.

Isolation and screening of heavy metal resistant bacteria from wastewater: a study of heavy metal co-resistance and antibiotics resistance

2012 ◽  
Vol 66 (10) ◽  
pp. 2041-2048 ◽  
Author(s):  
Benmalek Yamina ◽  
Benayad Tahar ◽  
Fardeau Marie Laure
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vibrio Fischeri ◽  
Micrococcus Luteus ◽  
Toxicity Tests ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Resistant Species ◽  
Luminescence Inhibition ◽  
Heavy Metal Resistant

The uncontrolled discharges of wastes containing a large quantity of heavy metal create huge economical and healthcare burdens particularly for people living near that area. However, the bioremediation of metal pollutants from wastewater using metal-resistant bacteria is a very important aspect of environmental biotechnology. In this study, 13 heavy metal resistant bacteria were isolated from the wastewater of wadi El Harrach in the east of Algiers and characterized. These include zinc-, lead-, chromium- and cadmium-resistant bacteria. The metal-resistant isolates characterized include both Gram-negative (77%) and Gram-positive (23%) bacteria. The Minimum Inhibitory Concentration (MIC) of wastewater isolates against the four heavy metals was determined in solid media and ranged from 100 to 1,500 μg/ml. All the isolates showed co-resistance to other heavy metals and antibiotic resistance of which 15% were resistant to one antibiotic and 85% were multi- and bi-antibiotics resistant. The zinc-resistant species Micrococcus luteus was the much more heavy metal resistant. The results of toxicity tests on Vibrio fischeri showed that the DI50 (5 min) as low as 0.1 carried away luminescence inhibition greater than 50%.

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