Molecular systematics of Keratinophyton: the inclusion of species formerly referred to Chrysosporium and description of four new species

Abstract Four new Keratinophyton species (Ascomycota, Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) region, the combined data analysis of ITS and the nuclear large subunit (LSU) rDNA, and their phenotype. Based on ITS phylogeny, within the Keratinophyton clade, K. lemmensii is clustered with K. durum, K. hubeiense, K. submersum and K. siglerae, while K. gollerae, K. straussii and K. wagneri are resolved in a separate terminal cluster. All four new species can be well distinguished from other asexual morphs in the genus Keratinophyton based on phenotypical characteristics alone. Ten new combinations are proposed for Chrysosporium asexual morphs which are resolved in the monophyletic Keratinophyton clade.

Download Full-text

Four New Keratinophyton Species (Onygenaceae) From Europe

10.21203/rs.3.rs-74808/v1 ◽

2020 ◽

Author(s):

Roman Labuda ◽

Andreas Bernreiter ◽

Doris Hochenauer ◽

Christoph Schüller ◽

Alena Kubatova

Keyword(s):

Internal Transcribed Spacer ◽

Large Subunit ◽

Its Region ◽

Soil Samples ◽

The Other ◽

Lsu Rdna ◽

New Combinations ◽

Internal Transcribed Spacer Region ◽

Asexual Morphs ◽

Terminal Cluster

Abstract Four new Keratinophyton species (Ascomycota: Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) region, the nuclear large subunit (LSU) rDNA, and their phenotype. Within the Keratinophyton clade, K. lemmensii is clustered with K. durum, K. hubeiense, K. submersum and K. siglerae, while K. gollerae, K. straussii and K. wagneri are resolved in a separate terminal cluster along with K. minutisporosum. All four new species can be well distinguished from the other asexual taxa in the genus Keratinophyton based on phenotypical characteristics alone. Ten new combinations are proposed for all other Chrysosporium asexual morphs which are resolved in the monophyletic Keratinophyton clade.

Download Full-text

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽

10.11646/phytotaxa.409.4.1 ◽

2019 ◽

Vol 409 (4) ◽

pp. 189-201 ◽

Cited By ~ 1

Author(s):

MALKA SABA ◽

DANNY HAELEWATERS ◽

MUHAMMAD FIAZ ◽

ABDUL NASIR KHALID ◽

DONALD H. PFISTER

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Pine Forests ◽

Evolutionary Relationships ◽

Internal Transcribed Spacer Region ◽

Bayesian Inferences ◽

A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Download Full-text

Two new rock-inhabiting species of Cyphellophora from karst habitats in China

Phytotaxa ◽

10.11646/phytotaxa.397.1.2 ◽

2019 ◽

Vol 397 (1) ◽

pp. 23

Author(s):

WEI SUN ◽

BINGJIE LIU ◽

RONG FU ◽

XINGZHONG LIU ◽

MEICHUN XIANG

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Morphological Characteristics ◽

Its Region ◽

Closely Related Species ◽

Two New Species ◽

Rock Inhabiting Fungi ◽

Karst Habitats

During survey on rock-inhabiting fungi from karst habitats in Guizhou, China, two new species in Cyphellophora were discovered and identified. Phylogenetic analysis based on combined sequences of the nuclear large subunit (nucLSU) and internal transcribed spacer (ITS) region of ribosomal DNA revealed that the tested isolates, clustered into two clades that well affiliated in the genus of Cyphellophora. Morphological characteristics were also supported the estabolishment of the new species. Herewith Cyphellophora botryose sp. nov. and Cyphellophora guizhouensis sp. nov. were described and their differences from closely related species were discussed.

Download Full-text

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽

10.11646/phytotaxa.401.3.5 ◽

2019 ◽

Vol 401 (3) ◽

pp. 199

Author(s):

MALKA SABA ◽

DANNY HAELEWATERS ◽

MUHAMMAD FIAZ ◽

ABDUL NASIR KHALID ◽

DONALD H. PFISTER

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Pine Forests ◽

Evolutionary Relationships ◽

Internal Transcribed Spacer Region ◽

Bayesian Inferences ◽

A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Download Full-text

Inocybe griseorubida, a new species of Pseudosperma clade from tropical India

Phytotaxa ◽

10.11646/phytotaxa.221.2.6 ◽

2015 ◽

Vol 221 (2) ◽

pp. 166 ◽

Cited By ~ 5

Author(s):

K. P. Deepna Latha ◽

Patinjareveettil Manimohan

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Rna Polymerase Ii ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Similar Species ◽

Internal Transcribed Spacer Region ◽

Comprehensive Description ◽

Kerala State ◽

A New Species

Inocybe griseorubida sp. nov. is described from Kerala State, India. A comprehensive description, photographs, and comparisons with phenetically similar species are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (rpb2) gene of this species were sequenced and analyzed. Phylogenetic analysis of rpb2 sequences confirmed both the novelty of the species and its placement within the Pseudosperma clade.

Download Full-text

A new and intriguing brown-spored Leucocoprinus species

Phytotaxa ◽

10.11646/phytotaxa.479.1.3 ◽

2021 ◽

Vol 479 (1) ◽

pp. 44-54

Author(s):

JUAN M. SUAREZ ◽

ALEXANDRA M. GOTTLIEB ◽

BERNARDO E. LECHNER

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

Buenos Aires ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Nuclear Rdna ◽

Internal Transcribed Spacer Region ◽

A New Species

Leucocoprinus brunneosporus sp. nov., collected at the Santa Catalina Reserve (Buenos Aires, Argentina), is proposed as a new species. We performed separate phylogenetic analyses of the nuclear rDNA large subunit (28S) and the complete nuclear rDNA internal transcribed spacer region (ITS) to establish the placement of the new species in the phylogeny of the Agaricaceae. This new species has a macromorphology similar to that of Lc. birnbaumii, but the spore print is light brown. Detailed descriptions and illustrations of the macro- and microscopic characters are provided.

Download Full-text

Four new species of Entoloma subgenus Pouzarella from India

Phytotaxa ◽

10.11646/phytotaxa.307.2.1 ◽

2017 ◽

Vol 307 (2) ◽

pp. 101 ◽

Cited By ~ 2

Author(s):

K. N. ANIL RAJ ◽

PATINJAREVEETTIL MANIMOHAN

Keyword(s):

New Species ◽

Sequence Alignment ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Morphological Data ◽

Similar Species ◽

Pairwise Sequence Alignment ◽

Internal Transcribed Spacer Region ◽

Kerala State ◽

Different Parts

Four new species of Entoloma s.l. subgenus Pouzarella (Entoloma wayanadense, E. peechiense, E. silvanum, and E. tropicum) collected from different parts of Kerala State, India are documented here. Descriptions, photographs and comparisons with phenetically similar species are provided. Sequences of the nuclear ribosomal internal transcribed spacer region (ITS) and the nuclear ribosomal large subunit (nrLSU) region of the new species were obtained and used in BLASTn searches and pairwise sequence alignment to support the morphological data and the novelty of the species. A key to the species of Entoloma subg. Pouzarella known from Kerala is provided.

Download Full-text

Three new species of Calocybella from India based on morphology and molecular phylogeny

Phytotaxa ◽

10.11646/phytotaxa.255.2.2 ◽

2016 ◽

Vol 255 (2) ◽

pp. 133 ◽

Cited By ~ 4

Author(s):

K. P. DEEPNA LATHA ◽

K. N. ANIL RAJ ◽

THUSHARA CHEROLIL ◽

SHAHINA A. SHARAFUDHEEN ◽

PATINJAREVEETTIL MANIMOHAN

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Molecular Phylogeny ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Its Region ◽

First Record ◽

Three New Species ◽

Kerala State ◽

Blastn Search

Three new species of Calocybella, C. lohitha, C. babruka and C. swetha are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions of these species are provided along with photo-illustrations and comparisons with phenetically similar and phylogenetically related species. Nuclear ribosomal internal transcribed spacer (ITS) region of the three species were sequenced and analyzed. In addition, nuclear ribosomal large subunit (nrLSU) region of Calocybella swetha was sequenced and used in BLASTn search to find similar sequences. ITS-based phylogenetic analysis using Maximum likelihood (ML) method supported both the novelty of these species and their placement within the genus. The discovery of these three species forms the first record of Calocybella outside Europe.

Download Full-text

Two new species of Barssia from China

Phytotaxa ◽

10.11646/phytotaxa.374.2.4 ◽

2018 ◽

Vol 374 (2) ◽

pp. 129

Author(s):

YU-YAN XU ◽

LI-JIE GUO ◽

TING LI ◽

LI FAN

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

North China ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Internal Transcribed Spacer Region ◽

Two New Species ◽

Molecular Phylogenetic

Two new truffle species Barssia guozigouensis and B. luyashanensis are described and illustrated from North China based on morphological and molecular evidences. Morphologically, B. guozigouensis is diagnosed by its distinctly warty ascomata and solid gleba with small and irregular chambers, and B. luyashanensis is by its red brown ascomata with fine warts and gleba without chambers. Molecular phylogenetic analyses based on the ribosomal internal transcribed spacer region and 28S large subunit nrDNA supported the placement of the new species.

Download Full-text