Expression of miR-146a and miR-155 in Egyptian patients with Behçet’s disease: clinical significance and relationship with disease activity

Abstract Background Behçet’s disease (BD) is a systemic vasculitis disorder with multifactorial immunopathogenesis and associated with significant morbidity and mortality. MicroRNAs (miRNAs) are involved in the pathogenesis of inflammatory diseases. MiR-146 and miR-155 are known key regulators of immune response. This study was conducted to determine the expression of miRNA-146a and miRNA-155 in patients with BD and to link their possible association with the clinical manifestations and activity of this disease to evaluate their role as diagnostic or prognostic markers. A total of 60 patients with BD and 25 age- and gender-matched healthy controls were examined in a case-control study from October 2017 to September 2018 for the expression levels of miR-146a and miR-155 using singleplexTaqMan two-step stem loop quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR). Results Patients with BD had significantly lower miR-146a levels than control subjects (P < 0.001). Regarding the miR-155 expression level, no statistically significant differences were detected between patients and healthy controls (P = 0.736). The expression level of miR-146a showed no significant association with the different clinical manifestations of patients with BD. Conclusion This study suggests the possibility that miR-146a expression in patients with BD is involved in the pathogenesis of disease. Furthermore, it can be used as a diagnostic biomarker and a therapeutic target for BD in the future.

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Intestinal manifestations of Behçet's disease

Terapevticheskii arkhiv ◽

10.26442/00403660.2019.05.000247 ◽

2019 ◽

Vol 91 (5) ◽

pp. 111-119 ◽

Cited By ~ 1

Author(s):

R G Goloeva ◽

Z S Alekberova ◽

T A Lisitsyna ◽

E A Stepanova ◽

L P Orlova

Keyword(s):

Behçet’S Disease ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Systemic Vasculitis ◽

Clinical Manifestations ◽

Surgical Care ◽

Unknown Origin ◽

Massive Bleeding ◽

Fistula Formation ◽

Behcet's Disease

Behçet's disease (BD) is a systemic vasculitis of unknown origin, characterized by recurrences of the ulcerative process in the oral cavity and on the genitals, inflammatory damage of the eyes, joints, vessels and other organs. The severity and prognosis of BD determines organ pathology. Intestinal manifestations of BD (intestinal BD) are the least studied. Its verification in BD is complicated by the variety of clinical manifestations, their similarity with inflammatory bowel diseases, the lack of informative laboratory tests, pathognomonic endoscopic and histological signs. Intestinal BD can lead to serious complications (massive bleeding, intestinal perforation and fistula formation), which can not only significantly reduce the quality of the patient’s life, but also cause death. Treatment of intestinal BD is not standardized; it is mainly empirical and conducted courses. The purpose of therapy is to achieve clinical remission, healing of intestinal ulcers and prevention of surgery. The article presents a case of severe refractory intestinal BD, requiring twice emergency surgical care - removal of half and then the whole of the colon because of multiple perforations. A brief review of the literature is given and diagnostic difficulties of intestinal BD are discussed.

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Phosphorylation Modulates Survivin Function in Behcet’s Disease

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2020.033 ◽

2020 ◽

Vol 10 (2) ◽

pp. 278-283 ◽

Cited By ~ 1

Author(s):

Yasamin Pahlavan ◽

Naser Samadi ◽

Khalil Ansarin ◽

Alireza Khabbazi

Keyword(s):

Behçet’S Disease ◽

Western Blotting ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Survivin Expression ◽

Expression Level ◽

Healthy Controls ◽

Behcet's Disease ◽

Western Blotting Analysis ◽

Blotting Analysis

Purpose: Survivin is critical for proliferation, maturation, homeostasis and differentiation of effector and memory lymphocytes. In this study the baculoviral inhibitors of apoptosis proteins (IAPs) repeat containing 5 (BIRC5) mRNA, survivin, and phosphorylated survivin expression were evaluated in peripheral blood mononuclear cells (PBMCs), and plasma of patients with Behcet’s disease (BD). Methods: In this study, 26 Iranian Azari patients diagnosed with BD and 30 healthy controls were recruited. Total RNA was extracted from PBMCs. The expression level of survivin was measured by quantitative real-time polymerase chain reaction (PCR). Survivin plasma levels were measured using survivin Enzyme-linked immunosorbent assays. Also, western blotting analysis was performed to measure phosphorylated-survivin and survivin levels in PBMCs and plasma of patients with BD. Results: In a pilot study, we showed that BIRC5 gene expression increased in BD patients compared with healthy controls (P<0.05). Western blotting analysis indicated that there was an increase in phosphorylated survivin expression in PBMCs of BD patients. Our data from western blot analysis showed survivin level in plasma samples of BD patients was similar to healthy controls. No significant differences were observed between plasma survivin levels in the BD patients compared with control group (P>0.05). The expression of phosphorylated survivin at Thr34 in PBMCs of BD patients with active disease was increased. Plasma phosphorylated survivin levels in having BD patients were also downregulated compared to healthy individuals. Conclusion: Analysis of PBMCs indicated increasing expression level of phosphorylated survivin in PBMCs of BD patients. There was also a downregulation in phosphorylated survivin levels in plasma of BD patients.

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Behcet’s Disease: Is There Geographical Variation? A Review Far from the Silk Road

International Journal of Rheumatology ◽

10.1155/2015/945262 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 20

Author(s):

Nieves Marie Leonardo ◽

Julian McNeil

Keyword(s):

Behçet’S Disease ◽

Geographical Variation ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Wide Spectrum ◽

Systemic Vasculitis ◽

Middle Eastern ◽

Clinical Manifestations ◽

Behcet's Disease ◽

Cardiac Manifestations

Behcet’s Disease (BD) is a systemic vasculitis characterized by the triad of recurrent mouth and genital ulcers with eye involvement. To date there are no laboratory tests specific for the disease and diagnosis continues to remain on clinical grounds. Multiple criteria have been created as guides for diagnosis; however, given the wide spectrum of organ involvement, some cases remain undiagnosed. The diagnosis of Behcet’s Disease may only be made over time as the clinical manifestations emerge sometimes separated by months and even years. With an increased recognition of this disease it has become apparent that there is geographical variation in clinical manifestations. In particular cardiac manifestations are not seen commonly in Caucasians compared to Asian and Middle Eastern patients, while neurological manifestations are more common in Caucasians. Use of immunosuppressive and immunomodulatory drugs to suppress inflammation remains the cornerstone of treatment.

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Circulating intercellular adhesion molecules in blood and bronchoalveolar lavage in Behçet's disease

Mediators of Inflammation ◽

10.1155/s0962935195000573 ◽

1995 ◽

Vol 4 (5) ◽

pp. 355-358 ◽

Cited By ~ 2

Author(s):

A. Hamzaoui ◽

K. Hamzaoui ◽

A. Chabbou ◽

K. Ayed

Keyword(s):

Bronchoalveolar Lavage ◽

Behçet’S Disease ◽

Inflammatory Process ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Clinical Manifestations ◽

Intercellular Adhesion ◽

Healthy Controls ◽

Behcet's Disease ◽

Immune System Activation

The aim of this study was to evaluate circulating intercellular adhesion molecule-1 (cICAM-1) in serum and in bronchoalveolar lavage (BAL), as a marker for the inflammatory process in patients with active Behçet's disease (BD). Circulating ICAM-1 was tested by an enzyme linked immuno-sorbent assay in serum and in BAL of patients with BD. These values were compared to those of patients with tuberculosis and to healthy controls. Increased levels of circulating ICAM-1 were found in serum from patients with active BD compared to healthy controls (p < 0.01). Similar levels of serum cICAM-1 were found in BD and tuberculosis. Additionally, both BD and tuberculosis patients exhibited high levels of cICAM-1 in BAL fluid, suggesting that this increase may be a result of the immune system activation in inflammatory sites. Circulating ICAM-1 seemed to have a good discriminative power in identifying active BD, being elevated in all active stages (p < 0.01) compared to remission BD stage. No differences were found in active BD patients depending upon the clinical manifestations. These results suggest that cICAM-1 may be involved in leucocyte adhesion and migration into the vessel wall of the lung. Circulating forms are derived from molecules expressed on the surface of activated cells, as a result of an inflammatory process.

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Old and New Challenges in Uveitis Associated with Behçet’s Disease

Journal of Clinical Medicine ◽

10.3390/jcm10112318 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2318

Author(s):

Julie Gueudry ◽

Mathilde Leclercq ◽

David Saadoun ◽

Bahram Bodaghi

Keyword(s):

Behçet’S Disease ◽

Structural Damage ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Systemic Vasculitis ◽

Retinal Vasculitis ◽

Unknown Origin ◽

Current Data ◽

Ocular Involvement ◽

Behcet's Disease

Behçet’s disease (BD) is a systemic vasculitis disease of unknown origin occurring in young people, which can be venous, arterial or both, classically occlusive. Ocular involvement is particularly frequent and severe; vascular occlusion secondary to retinal vasculitis may lead to rapid and severe loss of vision. Biologics have transformed the management of intraocular inflammation. However, the diagnosis of BD is still a major challenge. In the absence of a reliable biological marker, diagnosis is based on clinical diagnostic criteria and may be delayed after the appearance of the onset sign. However, therapeutic management of BD needs to be introduced early in order to control inflammation, to preserve visual function and to limit irreversible structural damage. The aim of this review is to provide current data on how innovations in clinical evaluation, investigations and treatments were able to improve the prognosis of uveitis associated with BD.

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Quality of life and diet: A paired match study on Behçet’s disease

Mediterranean Journal of Nutrition and Metabolism ◽

10.3233/mnm-211571 ◽

2022 ◽

pp. 1-11

Author(s):

Murat Urhan ◽

Reci Meseri ◽

Fahrettin Oksel

Keyword(s):

Quality Of Life ◽

Behçet’S Disease ◽

Diet Quality ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Short Form ◽

Activity Levels ◽

Healthy Controls ◽

Behcet's Disease

BACKGROUND: It has been reported that the quality of life and diet quality in individuals with rheumatological diseases are poor and may adversely affect the course of the disease. OBJECTIVE: This study aims to compare the quality of life and diet of individuals with Behçet’s Disease (BD) compared to healthy controls. METHODS: This study was planned as a case-control study, and 60 adult patients with BD were compared with age (±1) and sex paired match healthy controls concerning the quality of life and diet. Diet quality was assessed using nutrient adequacy ratio (NAR) and the mean adequacy ratio (MAR) values calculated from 24 h dietary food recall and obesity was also evaluated by various anthropometric measurements. The Short Form-36 Health Survey (SF-36) was used to evaluate the quality of life (QoL). In addition, the 24-hour physical activities were recorded to calculate physical activity levels (PAL). Data were analyzed by SPSS 25.0 via paired sample t-test and McNemar (and McNemar-Bowkers) test. p < 0.05 was deemed significant. RESULTS: The findings showed that cases were more obese (p = 0.005), less physically active (p < 0.001), had lower QoL (p < 0.01 for all subscales) and had higher Beck depression scores (p = 0.001). Controls had higher means of energy (p < 0.001), CHO% (p = 0.025), fat% (p = 0.004), and fiber (p = 0.007) intake and mean MAR value (p < 0.001). CONCLUSIONS: Compared to healthy controls, patients with BD were more obese, had lower QoL and lower diet quality. Therefore, people with BD should be evaluated for comorbid diseases and be supported by health professionals, such as dietitians and psychologists.

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Determination of mir-155 and mir-146a expression rates and its association with expression level of TNF-α and CTLA4 genes in patients with Behcet’s disease

Immunology Letters ◽

10.1016/j.imlet.2018.10.012 ◽

2018 ◽

Vol 204 ◽

pp. 55-59 ◽

Cited By ~ 12

Author(s):

Sousan Kolahi ◽

Mohammad-Jahed Farajzadeh ◽

Shahriar Alipour ◽

Alireza Abhari ◽

Jafar Farhadi ◽

...

Keyword(s):

Behçet’S Disease ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Expression Level ◽

Behcet's Disease ◽

Tnf Α

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Behcet’s Disease: An In-depth Review About Pathogenesis, Gastrointestinal Manifestations And Management

Inflammatory Intestinal Diseases ◽

10.1159/000520696 ◽

2021 ◽

Author(s):

Anthony Nguyen ◽

Shubhra Upadhyay ◽

Muhammad Ali Javaid ◽

Abdul Moiz Qureshi ◽

Shahan Haseeb ◽

...

Keyword(s):

Behçet’S Disease ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Age Groups ◽

Clinical Manifestations ◽

Signs And Symptoms ◽

Vascular Disorder ◽

Robust Algorithms ◽

Behcet's Disease ◽

Relapsing Remitting

Background: Behcet’s Disease (BD) is a complex inflammatory vascular disorder that follows a relapsing-remitting course with diverse clinical manifestations. The prevalence of the disease varies throughout the globe and targets different age groups. There are many variations of BD, however, intestinal BD is not only more common but has many signs and symptoms. Summary: BD is a relapsing-remitting inflammatory vascular disorder with multiple system involvement, affecting vessels of all types and sizes that targets young adults. The etiology of BD is unknown but many factors including genetic mechanisms, vascular changes, hypercoagulability and dysregulation of immune function are believed to be responsible. BD usually presents with signs and symptoms of ulcerative disease of the small intestine; endoscopy being consistent with the clinical manifestations. The mainstay of treatment depends upon the severity of the disease. Corticosteroids are recommended for severe forms of the disease and aminosalicylic acids are used in maintaining remission in mild to moderate forms of the disease. Key messages: In this review, we have tried to summarize in the present review the clinical manifestations, differential diagnoses and management of intestinal BD. Hopefully, this review will enable health policymakers to ponder over establishing clear endpoints for treatment, surveillance investigations and creating robust algorithms.

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Acute Agitation as an Initial Manifestation of Neuro-Behçet’s Disease

Case Reports in Emergency Medicine ◽

10.1155/2018/5437027 ◽

2018 ◽

Vol 2018 ◽

pp. 1-3

Author(s):

Yuki Otsuka ◽

Tetsuya Yumoto ◽

Hiromi Ihoriya ◽

Namiko Matsumoto ◽

Kota Sato ◽

...

Keyword(s):

Behçet’S Disease ◽

Behcet’S Disease ◽

Parietal Lobe ◽

Psychiatric Symptoms ◽

Behçet's Disease ◽

Physical Restraint ◽

Clinical Manifestations ◽

Initial Manifestation ◽

Behcet's Disease ◽

Acute Agitation

Managing acutely agitated or violent patients in the emergency department (ED) represents a significant challenge. Acute agitation as an initial manifestation of neuro-Behcet’s disease (NBD) is an extremely rare clinical entity. A 44-year-old male, who had been complaining about a severe headache and fever for several days, was admitted to our ED due to acutely presented incontinence and agitation. On admission, physical restraint and sedation with sevoflurane and propofol were required for his combative and violent behavior. Cerebrospinal fluid examination revealed increased cell count. Fluid attenuated inversion recovery magnetic resonance imaging showed a high intensity signal in the left parietal lobe and bilateral occipital lobe. As infectious meningoencephalitis was suspected, empirical therapy was immediately started. He recovered uneventfully without neurological defect in seven days. Based on positive human leukocyte antigen B-51 and clinical manifestations, the diagnosis of NBD was made and remitted by steroid therapy. Although acute NBD commonly presents with focal neurological symptoms, psychiatric symptoms could be considered the first manifestation. A focused and thorough examination coupled with appropriate management strategies can assist emergency clinicians safely and effectively manage these patients.

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Leukocyte toll-like receptor expression in pathergy positive and negative Behçet’s disease patients

Rheumatology ◽

10.1093/rheumatology/keaa251 ◽

2020 ◽

Vol 59 (12) ◽

pp. 3971-3979

Author(s):

Tim B van der Houwen ◽

Willem A Dik ◽

Marco Goeijenbier ◽

Manizhah Hayat ◽

Nicole M A Nagtzaam ◽

...

Keyword(s):

T Lymphocytes ◽

Behçet’S Disease ◽

Behcet’S Disease ◽

Behçet's Disease ◽

Receptor Expression ◽

Healthy Controls ◽

Toll Like Receptor ◽

Behcet's Disease ◽

Tnf Α ◽

Classical Monocytes

Abstract Objectives To investigate whether the auto-inflammatory nature and the pathergic reaction in Behçet’s disease (BD) are driven by a disturbed toll-like receptor (TLR) response. Methods We compared both TLR expression by flow-cytometry and TLR response by stimulation assay in 18 BD patients (both pathergy positive and pathergy negative) with 15 healthy controls. Results Expression of TLR1 and 2 was significantly elevated in B-lymphocytes of BD patients compared with healthy controls. TLR1, 2 and 4 were significantly more highly expressed in both CD4+ and CD8+ T-lymphocytes of BD patients. Granulocytes of BD patients displayed significantly higher expression of TLR1, 2, 4 and 6. TLR2, 4 and 5 expression was significantly increased on classical monocytes of BD patients. Intermediate monocytes of BD patients showed an increase in expression of TLR2. Furthermore, TLR2 and 5 were significantly more highly expressed in non-classical monocytes of BD patients. In pathergy positive patients, TLR5 was even more highly expressed compared with pathergy negative patients on B- and T-lymphocytes and granulocytes. Furthermore, TLR2 and 5 showed an elevated TNF-α response to stimulation with their cognate ligands. Conclusion Immune cells of BD patients overexpress TLR1, 2, 4, 5 and 6. Furthermore, after stimulation of TLR2 and 5, BD patients demonstrate a more potent TNF-α response. Although this is a small cohort, in the pathergy positive patients, TLR5 expression is even further augmented, suggesting that a microbial (flagellin) or damage (HMGB1) associated signal may trigger the exaggerated immune response that is characteristic for the pathergy phenomenon in BD. In conclusion, these results point to an exaggerated TLR response in the auto-inflammatory nature of BD.

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