scholarly journals Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

2013 ◽  
Vol 4 (5) ◽  
pp. 125 ◽  
Author(s):  
Andreia Ribeiro ◽  
Paula Laranjeira ◽  
Sandrine Mendes ◽  
Isabel Velada ◽  
Cristiana Leite ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
T Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Natural Killer ◽  
Umbilical Cord ◽  
Peripheral Blood ◽  
Umbilical Cord Matrix

Co-Transplantation of Autologous Umbilical Cord Matrix Mesenchymal Stem Cells Improves Engraftment of Umbilical Cord Blood in NOD/SCID Mice.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2569-2569
Author(s):  
Robb Friedman ◽  
Monica Betancur ◽  
Hande Tuncer ◽  
Laurent Boissel ◽  
Curtis Cetrulo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Peripheral Blood ◽  
Scid Mice ◽  
Umbilical Cord Matrix ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Abstract Umbilical cord blood (UCB) is a viable source of hematopoietic stem cells for transplantation of children and adults undergoing treatment for hematological malignancies. However only 4% of adults 70kg and over have a UCB unit available which contains the widely accepted minimum cell dose of 1.5x107 mononuclear cells per kilogram. Co-transplantation of hematopoietic stem cells with mesenchymal stem cells may enhance engraftment and therefore decrease transplant-related morbidity and mortality from delayed leukocyte recovery associated with a low pre-transplant cell dose. Umbilical cord matrix (UCM) cells, found in the Wharton’s Jelly, were easily and reliably extracted from minced pieces of cord by culture in RPMI + 20% fetal bovine serum at 37° and 5% humidified CO2. UCM expand best in 20% FBS but can also be expanded in human serum, autologous serum, and X-VIVO10. Small (1–3mm) minced pieces of umbilical cord can be cyropreserved at the time of delivery in 10% DMSO solution. UCM cells exhibit a fibroblast morphology and express markers common to mesenchymal stem cells: CD73 (SH3), CD105 (SH2), CD 29, CD44, CD49b, CD117, CD166, STRO-1 and HLA-DR. UCM are negative for CD14, CD 19, CD34, and CD45. Morphology and cell surface marker expression is stable after greater than fifteen passages. UCM cells grown in culture were shown to produce more GM-CSF and G-CSF than similar numbers of adult bone marrow mesenchymal stem cells, GM-CSF 178 pg/mL versus 77 pg/mL and G-CSF 82.6 pg/mL versus 7.9 pg/mL. NOD/SCID mice treated with anti-NK 1.1 antibodies and irradiated with 350 cGy were injected with suboptimal (1x104) numbers of cord blood CD34+ cells with and without 1x106 autologous UCM cells, extracted from the same umbilical cord as the cord blood CD34+ cells. Bone marrow was harvested at six weeks post transplant from both femurs and tibias and peripheral blood obtained via cardiac puncture. The percentage of human CD45+ cells in the bone marrow and the peripheral blood was assessed by flow cytometry. NOD/SCID mice transplanted with 1x104 cord blood CD34+ cells alone had 3.0% human CD45+ cell engraftment in the bone marrow and 3.6% human CD45+ cells in the peripheral blood, while NOD/SCID mice transplanted with 1x104 CD34+ cells and 1x106 UCM cells had an average of 27.3% human CD45+ cell engraftment in the bone marrow and 3.9% human CD45+ cells in the peripheral blood. These results indicate a trend towards improved engraftment in vivo with co-transplantation of suboptimal numbers of umbilical cord blood CD34+ cells and autologous umbilical cord matrix cells versus transplantation of suboptimal numbers of umbilical cord CD34+ cells alone.

Mesenchymal Stem Cells are of Recipient Origin in Pediatric Transplantations Using Umbilical Cord Blood, Peripheral Blood, or Bone Marrow

2007 ◽  
Vol 29 (6) ◽  
pp. 388-392 ◽  
Author(s):  
Javier Garc??a-Castro ◽  
Antonio Balas ◽  
Manuel Ram??rez ◽  
Antonio P??rez-Mart??nez ◽  
Luis Madero ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood

scholarly journals Potential of Human Umbilical Cord Matrix and Rabbit Bone Marrow–Derived Mesenchymal Stem Cells in Repair of Surgically Incised Rabbit External Anal Sphincter

2009 ◽  
Vol 52 (10) ◽  
pp. 1753-1761 ◽  
Author(s):  
Mahmoud Aghaee-afshar ◽  
Mohammad Rezazadehkermani ◽  
Alireza Asadi ◽  
Reza Malekpour-afshar ◽  
Armita Shahesmaeili ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Anal Sphincter ◽  
Umbilical Cord ◽  
External Anal Sphincter ◽  
Human Umbilical Cord ◽  
Umbilical Cord Matrix ◽  
Rabbit Bone

Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue

2008 ◽  
Vol 233 (7) ◽  
pp. 901-913 ◽  
Author(s):  
C. K. Rebelatto ◽  
A. M. Aguiar ◽  
M. P. Moretão ◽  
A. C. Senegaglia ◽  
P. Hansen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential

2014 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Danielle Barberini ◽  
Natália Pereira Freitas ◽  
Mariana Magnoni ◽  
Leandro Maia ◽  
Amanda Listoni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Differentiation Potential ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue

scholarly journals Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue

Stem Cells ◽  
2006 ◽  
Vol 24 (5) ◽  
pp. 1294-1301 ◽  
Author(s):  
Susanne Kern ◽  
Hermann Eichler ◽  
Johannes Stoeve ◽  
Harald Klüter ◽  
Karen Bieback
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Comparative Analysis ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood
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