scholarly journals Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods

2014 ◽  
Vol 55 (11) ◽  
pp. 2343-2353 ◽  
Author(s):  
Christian Marsching ◽  
Richard Jennemann ◽  
Raphael Heilig ◽  
Hermann-Josef Gröne ◽  
Carsten Hopf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Mass Spectrometric

scholarly journals Quantitative imaging of selenoprotein with multi-isotope imaging mass spectrometry (MIMS)

2014 ◽  
Vol 46 (S1) ◽  
pp. 154-157 ◽  
Author(s):  
S.S. Tang ◽  
C. Guillermier ◽  
M. Wang ◽  
J. C. Poczatek ◽  
N. Suzuki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry

scholarly journals Multicenter Validation Study of Quantitative Imaging Mass Spectrometry

2019 ◽  
Vol 91 (9) ◽  
pp. 6266-6274 ◽  
Author(s):  
Jeremy A. Barry ◽  
Rima Ait-Belkacem ◽  
William M. Hardesty ◽  
Lydia Benakli ◽  
Clara Andonian ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Validation Study ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Multicenter Validation

scholarly journals Therapeutic Hypothermia Achieves Neuroprotection via a Decrease in Acetylcholine with a Concurrent Increase in Carnitine in the Neonatal Hypoxia-Ischemia

2015 ◽  
Vol 35 (5) ◽  
pp. 794-805 ◽  
Author(s):  
Toshiki Takenouchi ◽  
Yuki Sugiura ◽  
Takayuki Morikawa ◽  
Tsuyoshi Nakanishi ◽  
Yoshiko Nagahata ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Therapeutic Hypothermia ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Brain Regions ◽  
Acetyl Coa ◽  
Neuroprotective Effects ◽  
Neurologic Outcomes ◽  
Hypoxia Ischemia ◽  
The Brain

Although therapeutic hypothermia is known to improve neurologic outcomes after perinatal cerebral hypoxia-ischemia, etiology remains unknown. To decipher the mechanisms whereby hypothermia regulates metabolic dynamics in different brain regions, we used a two-step approach: a metabolomics to target metabolic pathways responding to cooling, and a quantitative imaging mass spectrometry to reveal spatial alterations in targeted metabolites in the brain. Seven-day postnatal rats underwent the permanent ligation of the left common carotid artery followed by exposure to 8% O2 for 2.5 hours. The pups were returned to normoxic conditions at either 38°C or 30°C for 3 hours. The brain metabolic states were rapidly fixed using in situ freezing. The profiling of 107 metabolites showed that hypothermia diminishes the carbon biomass related to acetyl moieties, such as pyruvate and acetyl-CoA; conversely, it increases deacetylated metabolites, such as carnitine and choline. Quantitative imaging mass spectrometry demarcated that hypothermia diminishes the acetylcholine contents specifically in hippocampus and amygdala. Such decreases were associated with an inverse increase in carnitine in the same anatomic regions. These findings imply that hypothermia achieves its neuroprotective effects by mediating the cellular acetylation status through a coordinated suppression of acetyl-CoA, which resides in metabolic junctions of glycolysis, amino-acid catabolism, and ketolysis.

scholarly journals Protocol for Quantitative Imaging Mass Spectrometry

2016 ◽  
Vol 65 (12) ◽  
pp. 745-750
Author(s):  
Shuichi SHIMMA ◽  
Emi TAKEO ◽  
Eiichiro FUKUSAKI
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry

scholarly journals Paradoxical ATP Elevation in Ischemic Penumbra Revealed by Quantitative Imaging Mass Spectrometry

2010 ◽  
Vol 13 (8) ◽  
pp. 1157-1167 ◽  
Author(s):  
Katsuji Hattori ◽  
Mayumi Kajimura ◽  
Takako Hishiki ◽  
Tsuyoshi Nakanishi ◽  
Akiko Kubo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Ischemic Penumbra

scholarly journals Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry

Nitric Oxide ◽  
2015 ◽  
Vol 46 ◽  
pp. 102-113 ◽  
Author(s):  
Mitsuyo Ohmura ◽  
Takako Hishiki ◽  
Takehiro Yamamoto ◽  
Tsuyoshi Nakanishi ◽  
Akiko Kubo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Metabolic Systems

scholarly journals Quantitative imaging of inositol distribution in yeast using multi-isotope imaging mass spectrometry (MIMS)

2014 ◽  
Vol 46 (S1) ◽  
pp. 169-172 ◽  
Author(s):  
A. Saiardi ◽  
C. Guillermier ◽  
O. Loss ◽  
J. C. Poczatek ◽  
C. Lechene
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry
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