Quantitative imaging of cells with multi-isotope imaging mass spectrometry (MIMS)—Nanoautography with stable isotope tracers

2006 ◽  
Vol 252 (19) ◽  
pp. 6895-6906 ◽  
Author(s):  
Greg McMahon ◽  
Brian J. Glassner ◽  
Claude P. Lechene
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Stable Isotope Tracers ◽  
Isotope Tracers

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

2018 ◽  
Author(s):  
Shazia Khan ◽  
Diego F Cobice ◽  
Dawn EW Livingstone ◽  
C Logan Mackay ◽  
Scott P Webster ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Maldi Mass Spectrometry ◽  
Time Dependent ◽  
Stable Isotope Tracers ◽  
Isotope Tracers

scholarly journals Tracing Cationic Nutrients from Xylem into Stem Tissue of French Bean by Stable Isotope Tracers and Cryo-Secondary Ion Mass Spectrometry

2009 ◽  
Vol 152 (2) ◽  
pp. 1030-1043 ◽  
Author(s):  
Ralf Metzner ◽  
Heike Ursula Schneider ◽  
Uwe Breuer ◽  
Michael Robert Thorpe ◽  
Ulrich Schurr ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Secondary Ion Mass Spectrometry ◽  
French Bean ◽  
Stem Tissue ◽  
Stable Isotope Tracers ◽  
Isotope Tracers ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Nontargeted Elucidation of Metabolic Pathways Using Stable-Isotope Tracers and Mass Spectrometry

2010 ◽  
Vol 82 (15) ◽  
pp. 6621-6628 ◽  
Author(s):  
Karsten Hiller ◽  
Christian M. Metallo ◽  
Joanne K Kelleher ◽  
Gregory Stephanopoulos
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Metabolic Pathways ◽  
Stable Isotope Tracers ◽  
Isotope Tracers

scholarly journals Coupling APEX labeling to imaging mass spectrometry of single organelles reveals heterogeneity in lysosomal protein turnover

2019 ◽  
Vol 219 (1) ◽  
Author(s):  
Derek P. Narendra ◽  
Christelle Guillermier ◽  
Frank Gyngard ◽  
Xiaoping Huang ◽  
Michael E. Ward ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Cell Biology ◽  
Isotope Labeling ◽  
Imaging Mass Spectrometry ◽  
Lateral Resolution ◽  
Dynamic State ◽  
Metabolic Labeling ◽  
Isotope Tracers ◽  
Lysosomal Protein

Quantification of stable isotope tracers after metabolic labeling provides a snapshot of the dynamic state of living cells and tissue. A form of imaging mass spectrometry quantifies isotope ratios with a lateral resolution <50 nm, using a methodology that we refer to as multi-isotope imaging mass spectrometry (MIMS). Despite lateral resolution exceeding diffraction-limited light microscopy, lack of contrast has largely limited use of MIMS to large or specialized subcellular structures, such as the nucleus and stereocilia. In this study, we repurpose the engineered peroxidase APEX2 as the first genetically encoded marker for MIMS. Coupling APEX2 labeling of lysosomes and metabolic labeling of protein, we identify that individual lysosomes exhibit substantial heterogeneity in protein age, which is lost in iPSC-derived neurons lacking the lysosomal protein progranulin. This study expands the practical use of MIMS for cell biology by enabling measurements of metabolic function from stable isotope labeling within individual organelles in situ.

scholarly journals Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods

2014 ◽  
Vol 55 (11) ◽  
pp. 2343-2353 ◽  
Author(s):  
Christian Marsching ◽  
Richard Jennemann ◽  
Raphael Heilig ◽  
Hermann-Josef Gröne ◽  
Carsten Hopf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Mass Spectrometric

Novel and Nontraditional Use of Stable Isotope Tracers To Study Metal Bioavailability from Natural Particles

2013 ◽  
Vol 47 (7) ◽  
pp. 3424-3431 ◽  
Author(s):  
Marie-Noële Croteau ◽  
Daniel J. Cain ◽  
Christopher C. Fuller
Keyword(s):  
Stable Isotope ◽  
Metal Bioavailability ◽  
Stable Isotope Tracers ◽  
Isotope Tracers
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