scholarly journals Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry

Nitric Oxide ◽  
2015 ◽  
Vol 46 ◽  
pp. 102-113 ◽  
Author(s):  
Mitsuyo Ohmura ◽  
Takako Hishiki ◽  
Takehiro Yamamoto ◽  
Tsuyoshi Nakanishi ◽  
Akiko Kubo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Metabolic Systems

scholarly journals Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods

2014 ◽  
Vol 55 (11) ◽  
pp. 2343-2353 ◽  
Author(s):  
Christian Marsching ◽  
Richard Jennemann ◽  
Raphael Heilig ◽  
Hermann-Josef Gröne ◽  
Carsten Hopf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Mass Spectrometric

scholarly journals High-resolution imaging mass spectrometry combined with transcriptomic analysis identified a link between fatty acid composition of phosphatidylinositols and the immune checkpoint pathway at the primary tumour site of breast cancer

2019 ◽  
Vol 122 (2) ◽  
pp. 245-257 ◽  
Author(s):  
Masahiro Kawashima ◽  
Mariko Tokiwa ◽  
Tomomi Nishimura ◽  
Yukiko Kawata ◽  
Masahiro Sugimoto ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Immune Checkpoint ◽  
Imaging Mass Spectrometry ◽  
Invasive Cancer ◽  
Fatty Acid Binding ◽  
Checkpoint Pathway ◽  
Maldi Ims

Abstract Background The fatty acid (FA) composition of phosphatidylinositols (PIs) is tightly regulated in mammalian tissue since its disruption impairs normal cellular functions. We previously found its significant alteration in breast cancer by using matrix-assisted laser desorption and ionisation imaging mass spectrometry (MALDI-IMS). Methods We visualised the histological distribution of PIs containing different FAs in 65 primary breast cancer tissues using MALDI-IMS and investigated its association with clinicopathological features and gene expression profiles. Results Normal ductal cells (n = 7) predominantly accumulated a PI containing polyunsaturated FA (PI-PUFA), PI(18:0/20:4). PI(18:0/20:4) was replaced by PIs containing monounsaturated FA (PIs-MUFA) in all non-invasive cancer cells (n = 12). While 54% of invasive cancer cells (n = 27) also accumulated PIs-MUFA, 46% of invasive cancer cells (n = 23) accumulated the PIs-PUFA, PI(18:0/20:3) and PI(18:0/20:4). The accumulation of PI(18:0/20:3) was associated with higher incidence of lymph node metastasis and activation of the PD-1-related immune checkpoint pathway. Fatty acid-binding protein 7 was identified as a putative molecule controlling PI composition. Conclusions MALDI-IMS identified PI composition associated with invasion and nodal metastasis of breast cancer. The accumulation of PI(18:0/20:3) could affect the PD-1-related immune checkpoint pathway, although its precise mechanism should be further validated.

scholarly journals Development of Laser Ionization Imaging Mass Spectrometry for Multiple Drugs Administered to Cancer Cells

2015 ◽  
Vol 21 (S3) ◽  
pp. 2231-2232
Author(s):  
Hisanao Hazama ◽  
Hiroki Kannen ◽  
Jun Aoki ◽  
Michisato Toyoda ◽  
Tatsuya Fujino ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Imaging Mass Spectrometry ◽  
Laser Ionization ◽  
Multiple Drugs

scholarly journals Quantitative imaging of selenoprotein with multi-isotope imaging mass spectrometry (MIMS)

2014 ◽  
Vol 46 (S1) ◽  
pp. 154-157 ◽  
Author(s):  
S.S. Tang ◽  
C. Guillermier ◽  
M. Wang ◽  
J. C. Poczatek ◽  
N. Suzuki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry

scholarly journals Multicenter Validation Study of Quantitative Imaging Mass Spectrometry

2019 ◽  
Vol 91 (9) ◽  
pp. 6266-6274 ◽  
Author(s):  
Jeremy A. Barry ◽  
Rima Ait-Belkacem ◽  
William M. Hardesty ◽  
Lydia Benakli ◽  
Clara Andonian ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Validation Study ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Multicenter Validation

scholarly journals Therapeutic Hypothermia Achieves Neuroprotection via a Decrease in Acetylcholine with a Concurrent Increase in Carnitine in the Neonatal Hypoxia-Ischemia

2015 ◽  
Vol 35 (5) ◽  
pp. 794-805 ◽  
Author(s):  
Toshiki Takenouchi ◽  
Yuki Sugiura ◽  
Takayuki Morikawa ◽  
Tsuyoshi Nakanishi ◽  
Yoshiko Nagahata ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Therapeutic Hypothermia ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry ◽  
Brain Regions ◽  
Acetyl Coa ◽  
Neuroprotective Effects ◽  
Neurologic Outcomes ◽  
Hypoxia Ischemia ◽  
The Brain

Although therapeutic hypothermia is known to improve neurologic outcomes after perinatal cerebral hypoxia-ischemia, etiology remains unknown. To decipher the mechanisms whereby hypothermia regulates metabolic dynamics in different brain regions, we used a two-step approach: a metabolomics to target metabolic pathways responding to cooling, and a quantitative imaging mass spectrometry to reveal spatial alterations in targeted metabolites in the brain. Seven-day postnatal rats underwent the permanent ligation of the left common carotid artery followed by exposure to 8% O2 for 2.5 hours. The pups were returned to normoxic conditions at either 38°C or 30°C for 3 hours. The brain metabolic states were rapidly fixed using in situ freezing. The profiling of 107 metabolites showed that hypothermia diminishes the carbon biomass related to acetyl moieties, such as pyruvate and acetyl-CoA; conversely, it increases deacetylated metabolites, such as carnitine and choline. Quantitative imaging mass spectrometry demarcated that hypothermia diminishes the acetylcholine contents specifically in hippocampus and amygdala. Such decreases were associated with an inverse increase in carnitine in the same anatomic regions. These findings imply that hypothermia achieves its neuroprotective effects by mediating the cellular acetylation status through a coordinated suppression of acetyl-CoA, which resides in metabolic junctions of glycolysis, amino-acid catabolism, and ketolysis.

scholarly journals Protocol for Quantitative Imaging Mass Spectrometry

2016 ◽  
Vol 65 (12) ◽  
pp. 745-750
Author(s):  
Shuichi SHIMMA ◽  
Emi TAKEO ◽  
Eiichiro FUKUSAKI
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Imaging ◽  
Imaging Mass Spectrometry
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