scholarly journals Expression of Immune Response Markers in Arab Patients With Lung Cancer

2020 ◽  
pp. 1218-1224
Author(s):  
Abdul Rahman Jazieh ◽  
Adda Bounedjar ◽  
Hanaa Bamefleh ◽  
Turki Alfayea ◽  
Hatim Q. Almaghraby ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Univariate Analysis ◽  
Tumor Infiltrating Lymphocytes ◽  
High Expression ◽  
Arab Population ◽  
Cd8 Cells ◽  
Programmed Death ◽  
Pathologic Features ◽  
Cluster Of Differentiation ◽  
Infiltrating Lymphocytes

PURPOSE Programmed death-ligand 1 (PD-L1) is a marker for checkpoint inhibitor use in the management of solid tumors, especially in non–small-cell lung cancer (NSCLC). Our study was aimed at determining the patterns of PD-L1 expression and cluster of differentiation 8 (CD8) immunostains in patients with NSCLC in the Arab population. METHODS Archival tumor tissue from patients with a confirmed diagnosis of NSCLC were obtained and stained for PD-L1 with antibody 22C3, using immunohistochemistry staining and giving the tumor proportion score (TPS) as a percentage from 0%-100% of stained tumor cells. Tumors were categorized into negative expressers (TPS < 1%), low positive (TPS, 1%-49%), and high positive (TPS, 50%-100%). Correlation of expression with clinical and pathologic features, including CD8-positive (CD8+) lymphocyte density, was also analyzed. RESULTS Two hundred patients with NSCLC were included in the study from 6 centers in Saudi Arabia and Algeria. Median age was 65 years (28-93 years), and the majority were men (75%) with stage 4 NSCLC (64%). The TPS was high in 37 patients (18%), low in 60 patients (30%), and negative in 103 patients (52%). In a univariate analysis, the following were significant predictors of any PD-L1 expression (> 1%): male sex, being Saudi national patients, high expression of CD8+, and presence of tumor-infiltrating lymphocytes. In the multivariate analysis, only high expression of CD8+ cells (≥ 2+) was significant, with an odds ratio of 4.4 (95% CI, 1.5 to 12.9; P = .003) CONCLUSION PD-L1 expression in our population is similar to the published literature and correlated with the density of CD8+ cells. Validation of the predictive value of this marker in our population and identifying easier and reliable methods to test for it are warranted.

Expression of immune response markers in Arab patients with lung cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e21543-e21543
Author(s):  
Abdul Rahman Jazieh ◽  
Adda Bounedjar ◽  
Hanaa Bamefleh ◽  
Turki Al-Fayea ◽  
Hatim Q. Almaghraby ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Checkpoint Inhibitors ◽  
Univariate Analysis ◽  
Tumor Infiltrating Lymphocytes ◽  
High Expression ◽  
P Value ◽  
Arab Population ◽  
Cd8 Cells ◽  
Immuno Histochemistry ◽  
Infiltrating Lymphocytes

e21543 Background: Programmed death-ligand 1 (PD-L1) is a marker for checkpoint inhibitors use in management of solid tumor especially non-small cell lung cancer (NSCLC). Our study aimed at determining the patterns of PD-L1 expression and CD8 immunostains in patients with NSCLC in Arab population. Methods: Archival tumor tissue form patients with confirmed diagnosis of NCSLC were obtained and stained for PD-L1 using antibody 22C3 by Dako using Immuno-histochemistry stain (IHC) and giving Tumor Proportion Score (TPS) a percentage number from 0 to 100% of stained tumor cells. Tumors were categorized into negative expressers (TPS<1%), low positive (TPS 1-49%), and high positive (TPS 50% -100%). Correlation of expression with clinical and pathological features including CD8+ lymphocyte density was analyzed. Results: Two hundred NSCLC cases were included in the study from 6 centers in Saudi Arabia and Algeria. Median age was 65 years (28-93) and majority were males (75%) with stage 4 NSCLC (64%). The TPS was high in 37 patients (18%), low in 60 patients (30%), and negative in 103 patients (52%). In a univariate analysis, the following were significant predictors of any PD-L1 expression (>1%): male gender, being Saudi national patients, high expression of CD8+ and the presence of tumor infiltrating lymphocytes. In the multivariate analysis, only high expression of CD8+ cells (>2+) was significant with Odd Ratio (OR) of 4.4 (95% Confidence interval 1.5-12.9, p value of 0.003). Conclusions: PD-L1 expression in our population is similar to the published literature and correlated with the density of CD8+ cells. Validation of the predictive value of this marker in our population and identifying easier and reliable methods to test for it is warranted.

scholarly journals High expression of PD-L1 Predicts Worse Overall Survival in the Cavitary Lung Adenocarcinoma

2020 ◽  
Author(s):  
Jiangyong Liu ◽  
Mingming Gu ◽  
Yang Xue ◽  
Yong Ren ◽  
Wencai Huang
Keyword(s):  
Lung Cancer ◽  
Overall Survival ◽  
Lung Adenocarcinoma ◽  
Prognostic Significance ◽  
Tumor Infiltrating Lymphocytes ◽  
Good Prognosis ◽  
High Expression ◽  
Quantitative Immunofluorescence ◽  
L1 Protein ◽  
Infiltrating Lymphocytes

Abstract Objective Solitary cavitary lung cancer is one of the rare types of lung cancer. Generally, the relationship between cavitary lung adenocarcinoma and immunotherapy remains unknown. We aimed to assess programmed cell death ligand-1(PD-L1) expression and CD8-positive (CD8+) tumor infiltrating lymphocytes (TILs) density, and evaluate their prognostic significance of patients with cavitary lung adenocarcinoma (LUAD). Methods 65 patients diagnosed as solitary cavitary LUAD were included in this study, 30 cases of noncavitary LUAD patients were collected as controls, and their specimens from surgery or biopsy were obtained. Expression of PD-L1 protein and CD8+ TILs were detected by traditional immunohistochemistry and multiplex quantitative immunofluorescence technology. The correlations of PD-L1 expression and clinicopathological features, including overall survival in cavitary LUAD patients was evaluated based on the follow-up data. Results Overexpression of PD-L1 protein was detected in the tumor tissues of cavitary LUAD patients compared to the noncavitary LUAD controls. PD-L1 expression level was significantly related to the lymph node (P = 0.001), TNM stage (P = 0.024), and CD8+ TIL status (rs= -0.272, P = 0.025). High PD-L1 expression predicted high mortality rate (P < 0.001), but CD8+ TIL group showed better survival in cavitary LUAD patients (P = 0.011). This phenotype with high PD-L1 expression and low CD8 + TIL predicted poorer overall survival of the patients with cavitary LUAD, compared to the other phenotypes. Moreover, CD8+ TIL was an independent good prognosis factor. Conclusion We firstly demonstrated that PD-L1 is upregulated in the cavitary LUAD patients, and high expression of PD-L1 negatively correlated with CD8 T cell infiltrating status. High PD-L1 expression and low CD8 + TIL can predict poorer overall survival of the patients with cavitary LUAD.

scholarly journals Clinical Significance of Tumor Microenvironment in Acral Melanoma: A Large Single-Institution Study of Caucasians

2021 ◽  
Vol 10 (7) ◽  
pp. 1452
Author(s):  
Aneta Maria Borkowska ◽  
Anna Szumera-Ciećkiewicz ◽  
Maria Chraszczewska ◽  
Kamil Sokół ◽  
Tomasz Goryń ◽  
...  
Keyword(s):  
Univariate Analysis ◽  
Tumor Infiltrating Lymphocytes ◽  
Lymph Node Status ◽  
Survival Outcomes ◽  
Acral Melanoma ◽  
Kaplan Meier ◽  
Programmed Death ◽  
Impact On Survival ◽  
Melanoma Patients ◽  
Infiltrating Lymphocytes

Background: The presence of tumor-infiltrating lymphocytes (TILs) in many studies is associated with a better prognosis in melanoma patients. Programmed death-ligand 1 (PD-L1) expression has a significant value in predicting several cancers, but its role in melanoma remains ambiguous. The study aims to report a comprehensive analysis of TILs characteristics and their impact on survival in primary acral melanoma (AM). Methods: Clinical and pathological features and survival outcomes were investigated in 70 patients with AM. Immunohistochemical quantitative analysis of TILs, including expression of CD4, CD8, FOXP3, PD-1, and PD-L1, on melanoma cells was performed. Results: Kaplan-Meier analysis showed significant differences in overall survival (OS) for CD4+ (p = 0.021), CD8+ (p = 0.037), FOXP3+ (p = 0.007), and TILs density (p = 0.043). In univariate analysis of immunohistochemical features, FOXP3, CD4, CD8, PD-1, and Melanoma Institute of Australia (MIA) grading TILs (grade, density, and distribution) were correlated with survival. The higher density of FOXP3-positive cells was an independent factor associated with better survival. Conclusions: High TILs content (classed as brisk Clark scale and marked/diffuse TILs MIA grade) regardless of its immunophenotype was associated with better survival outcomes in AM. PD-L1 expression on tumor cells did not influence OS and was independent of clinical and pathological characteristics. We demonstrated that TILs are significant biomarkers in sentinel lymph node status prediction.

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