The proboscis extension response: assessing the sublethal effects of pesticides on the honey bee A . DECOURTYE , AND M . H . PHAM - DELÈGUE

Abstract Background Dinotefuran (CAS No. 165252–70-0), a neonicotinoid insecticide, has been used to protect various crops against invertebrate pests and has been associated with numerous negative sublethal effects on honey bees. Long noncoding RNAs (lncRNAs) play important roles in mediating various biological and pathological processes, involving transcriptional and gene regulation. The effects of dinotefuran on lncRNA expression and lncRNA function in the honey bee brain are still obscure. Results Through RNA sequencing, a comprehensive analysis of lncRNAs and mRNAs was performed following exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, and 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10, respectively. Gene set enrichment analysis (GSEA) indicated that the dinotefuran-treated group showed enrichment in carbohydrate and protein metabolism and immune-inflammatory responses such as glycine, serine and threonine metabolism, pentose and glucuronate interconversion, and Hippo and transforming growth factor-β (TGF-β) signaling pathways. Moreover, the DE lncRNA TCONS_00086519 was shown by fluorescence in situ hybridization (FISH) to be distributed mainly in the cytoplasm, suggesting that it may serve as a competing endogenous RNA and a regulatory factor in the immune response to dinotefuran. Conclusion This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees and provided a framework for further study of the role of lncRNAs in honey bee growth and the immune response.

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Responsiveness to sucrose and habituation of the proboscis extension response in honey bees

Journal of Comparative Physiology A ◽

10.1007/s00359-004-0531-6 ◽

2004 ◽

Vol 190 (9) ◽

Cited By ~ 32

Author(s):

Ricarda Scheiner

Keyword(s):

Honey Bees ◽

Proboscis Extension Response ◽

Proboscis Extension

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Developmental study of the Proboscis Extension Response to heat in Rhodnius prolixus along the life cycle

Journal of Insect Physiology ◽

10.1016/j.jinsphys.2016.11.010 ◽

2017 ◽

Vol 98 ◽

pp. 55-58 ◽

Cited By ~ 3

Author(s):

Carole Labrousse ◽

Claudio R. Lazzari ◽

Nadine Fresquet

Keyword(s):

Life Cycle ◽

Rhodnius Prolixus ◽

Developmental Study ◽

Proboscis Extension Response ◽

Proboscis Extension

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The proboscis extension response

Honey Bees ◽

10.1201/9780203218655.ch5 ◽

2002 ◽

pp. 67-84 ◽

Cited By ~ 2

Author(s):

M Pham-Del√®gue ◽

A Decourtye

Keyword(s):

Proboscis Extension Response ◽

Proboscis Extension

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Behavioral responses of the invasive fly Philornis downsi to stimuli from bacteria and yeast in the laboratory and the field in the Galapagos Islands

10.1101/696492 ◽

2019 ◽

Author(s):

Boaz Yuval ◽

Paola Lahuatte ◽

Arul J. Polpass ◽

Charlotte Causton ◽

Edouard Jurkevitch ◽

...

Keyword(s):

Integrated Management ◽

Gut Bacteria ◽

Growth Media ◽

Proboscis Extension Response ◽

Galapagos Archipelago ◽

Philornis Downsi ◽

Baited Traps ◽

Proboscis Extension ◽

Future Work ◽

Bird Feces

AbstractPhilornis downsi (Diptera: Muscidae) is a nest parasitic fly that has invaded the Galapagos archipelago and exerts an onerous burden on populations of endemic land birds. As part of an ongoing effort to develop tools for the integrated management of this fly, our objective was to determine its long and short-range responses to bacterial and yeast cues associated with adult P. downsi. We hypothesized that the bacterial and yeast communities will elicit attraction at distance through volatiles, and appetitive responses upon contact. Accordingly, we amplified bacteria from guts of adult field-caught individuals and bird feces, and yeasts from fermenting papaya juice (a known attractant of P. downsi), on selective growth media, and assayed the response of flies to these microbes or their exudates. In the field, we baited traps with bacteria or yeast and monitored adult fly attraction. In the laboratory, we used the Proboscis Extension Response (PER) to determine the sensitivity of males and females to tarsal contact with bacteria or yeast. Long range trapping efforts yielded two female flies over 112 trap nights (one in extracts from bird faeces and one in extracts from gut bacteria from adult flies). In the laboratory, tarsal contact with bacterial stimuli from gut bacteria from adult flies elicited significantly more responses than did yeast stimuli. We discuss the significance of these findings in context with other studies in the field and identify targets for future work.

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Seasonal Variation of Proboscis Extension Reflex Conditioning in the Honey Bee(Apis Mellifera)

Journal of Apicultural Research ◽

10.1080/00218839.1997.11100936 ◽

1997 ◽

Vol 36 (2) ◽

pp. 108-110 ◽

Cited By ~ 15

Author(s):

Stephen Ray ◽

Benjamin Ferneyhough

Keyword(s):

Seasonal Variation ◽

Apis Mellifera ◽

Honey Bee ◽

Proboscis Extension Reflex ◽

Proboscis Extension

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Behavioral Responses of the Invasive Fly Philornis downsi to Stimuli from Bacteria and Yeast in the Laboratory and the Field in the Galapagos Islands

Insects ◽

10.3390/insects10120431 ◽

2019 ◽

Vol 10 (12) ◽

pp. 431

Author(s):

Boaz Yuval ◽

Paola Lahuatte ◽

Polpass Arul Jose ◽

Charlotte E. Causton ◽

Edouard Jurkevitch ◽

...

Keyword(s):

Integrated Management ◽

Growth Media ◽

Proboscis Extension Response ◽

Galapagos Archipelago ◽

Philornis Downsi ◽

Baited Traps ◽

Proboscis Extension ◽

Males And Females ◽

Future Work ◽

Bird Feces

Philornis downsi Dodge and Aitken (Diptera: Muscidae) is an avian parasitic fly that has invaded the Galapagos archipelago and exerts an onerous burden on populations of endemic land birds. As part of an ongoing effort to develop tools for the integrated management of this fly, our objective was to determine its long- and short-range responses to bacterial and fungal cues associated with adult P. downsi. We hypothesized that the bacterial and fungal communities would elicit attraction at distance through volatiles, and appetitive responses upon contact. Accordingly, we amplified bacteria from guts of adult field-caught flies and from bird feces, and yeasts from fermenting papaya juice (a known attractant of P. downsi), on selective growth media, and assayed the response of flies to these microbes or their exudates. In the field, we baited traps with bacteria or yeast and monitored adult fly attraction. In the laboratory, we used the proboscis extension response (PER) to determine the sensitivity of males and females to tarsal contact with bacteria or yeast. Long range trapping efforts yielded two female flies over 112 trap-nights (attracted by bacteria from bird feces and from the gut of adult flies). In the laboratory, tarsal contact with stimuli from gut bacteria elicited significantly more responses than did yeast stimuli. We discuss the significance of these findings in context with other studies in the field and identify targets for future work.

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