Rice Transformation

2020 ◽  
pp. 119-188
Author(s):  
Paul Christou
Keyword(s):  
Rice Transformation

Rice Transformation: Methods and Applications

1995 ◽  
pp. 53-64
Author(s):  
Junko Kyozuka ◽  
Ko Shimamoto
Keyword(s):  
Rice Transformation ◽  
Transformation Methods

scholarly journals Efficient Genetic Transformation of Rice for CRISPR/Cas9 Mediated Genome-Editing and Stable Overexpression Studies: A Case Study on Rice Lipase 1 and Galactinol Synthase Encoding Genes

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 179
Author(s):  
Tanika Thakur ◽  
Kshitija Sinha ◽  
Tushpinder Kaur ◽  
Ritu Kapoor ◽  
Gulshan Kumar ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Crop Improvement ◽  
Rice Cultivar ◽  
Climatic Conditions ◽  
Rice Transformation ◽  
Transformation Protocol ◽  
Galactinol Synthase ◽  
Biotic Stresses ◽  
Putative Transformants ◽  
Efficient Genetic Transformation

Rice is a staple food crop for almost half of the world’s population, especially in the developing countries of Asia and Africa. It is widely grown in different climatic conditions, depending on the quality of the water, soil, and genetic makeup of the rice cultivar. Many (a)biotic stresses severely curtail rice growth and development, with an eventual reduction in crop yield. However, for molecular functional analysis, the availability of an efficient genetic transformation protocol is essential. To ensure food security and safety for the continuously increasing global population, the development of climate-resilient crops is crucial. Here, in this study, the rice transformation protocol has been effectively optimized for the efficient and rapid generation of rice transgenic plants. We also highlighted the critical steps and precautionary measures to be taken while performing the rice transformation. We further assess the efficacy of this protocol by transforming rice with two different transformation constructs for generating galactinol synthase (GolS) overexpression lines and CRISPR/Cas9-mediated edited lines of lipase (Lip) encoding the OsLip1 gene. The putative transformants were subjected to molecular analysis to confirm gene integration/editing, respectively. Collectively, the easy, efficient, and rapid rice transformation protocol used in this present study can be applied as a potential tool for gene(s) function studies in rice and eventually to the rice crop improvement.

Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium

Plant Science ◽  
2004 ◽  
Vol 166 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Zhengquan He ◽  
Yaping Fu ◽  
Huamin Si ◽  
Guocheng Hu ◽  
Shihong Zhang ◽  
...  
Keyword(s):  
Selectable Marker ◽  
Phosphomannose Isomerase ◽  
Rice Transformation

The relationship between PMI (manA) gene expression and optimal selection pressure in Indica rice transformation

2014 ◽  
Vol 33 (7) ◽  
pp. 1081-1090 ◽  
Author(s):  
Huaping Gui ◽  
Xia Li ◽  
Yubo Liu ◽  
Kai Han ◽  
Xianggan Li
Keyword(s):  
Gene Expression ◽  
Selection Pressure ◽  
Indica Rice ◽  
Optimal Selection ◽  
Rice Transformation ◽  
The Relationship

Initial function determination for the open reading frame (ORF) region of Pib gene via rice transformation

2007 ◽  
Vol 1 (3) ◽  
pp. 250-254
Author(s):  
Hui Yang ◽  
Shihu Yang ◽  
Qing Wang ◽  
Tong Zhou ◽  
Jianmin Wan
Keyword(s):  
Initial Function ◽  
Open Reading Frame ◽  
Rice Transformation ◽  
Reading Frame

Improvement of rice transformation using bombardment of scutellum-derived calli

2003 ◽  
Vol 21 (4) ◽  
pp. 429-437 ◽  
Author(s):  
Miguel Martinez-Trujillo ◽  
José Luis Cabrera-Ponce ◽  
Luis Herrera-Estrella
Keyword(s):  
Rice Transformation

scholarly journals Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens

2016 ◽  
Vol 5 (2) ◽  
pp. 49
Author(s):  
Atmitri Sisharmini ◽  
Aniversari Apriana ◽  
Wening Enggraini ◽  
Kurniawan R. Trijatmiko
Keyword(s):  
Insertional Mutagenesis ◽  
Agronomic Traits ◽  
Rice Genome ◽  
Sequence Information ◽  
Rice Transformation ◽  
Rice Genome Sequence ◽  
Rice Mutant ◽  
System A ◽  
Agrobacterium Vector ◽  
Genome Sequence Information

<p>The rice transformation technology<br />is not only provides valuable methods for the introduction<br />of useful genes into rice plant to improve important<br />agronomic traits, but also helps in studying gene function<br />and regulation based on rice genome sequence information.<br />Knockout of genes by insertional mutagenesis is a straightforward<br />method to identify gene functions. One of the<br />methods to develop rice mutants is through genetic transformation<br />mediated by Agrobacterium using activation<br />tagging by Ac-Ds system. A study was done with an objective<br />to obtain mutant rice of local tropical japonica cv. Asemandi<br />through genetic trans-formation mediated by Agrobacterium<br />tumefaciens. The transformation was conducted using<br />Agrobacterium vector with the strain of Agl-1 containing<br />activation tag construct. The result of experiment showed<br />that it has been obtained 17 independent line (304 plants)<br />transgenic Asemandi containing activation tag construct.<br />These starter lines will be used as materials to develop<br />several generations of stabil rice mutant through selfing.</p>

scholarly journals New chimeric promoter useful for expression of selectable marker genes in rice transformation

2005 ◽  
Vol 22 (4) ◽  
pp. 287-294
Author(s):  
Hiromi Higo ◽  
Kayo Tsuruya ◽  
Hironori Mano ◽  
Kana Hasegawa ◽  
Yuzo Minobe
Keyword(s):  
Selectable Marker ◽  
Marker Genes ◽  
Rice Transformation ◽  
Chimeric Promoter ◽  
Selectable Marker Genes

scholarly journals Rapid Agrobacterium-mediated genetic rice transformation method using liquid media

2013 ◽  
Vol 40 (1) ◽  
pp. 37-42
Author(s):  
Dae-Hwa Yang ◽  
Ahn-Cheol Chang ◽  
Il-Pyung Ahn ◽  
Hae-Jung Kim ◽  
Dong-Hern Kim ◽  
...  
Keyword(s):  
Transformation Method ◽  
Liquid Media ◽  
Rice Transformation

Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits

PROTOPLASMA ◽  
2019 ◽  
Vol 256 (4) ◽  
pp. 997-1011
Author(s):  
Pallavi Singh ◽  
Hussain Ara ◽  
Sumaira Tayyeba ◽  
Chandana Pandey ◽  
Alok Krishna Sinha
Keyword(s):  
Map Kinase ◽  
Phenotypic Traits ◽  
Rice Transformation
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