Rapid Enumeration of Airborne Microorganisms by Bioluminescent ATP Assay

Author(s):  
Jillian S. Deans ◽  
I. W. Stewart ◽  
T. T. Salusbury
2000 ◽  
Author(s):  
G. Mainelis ◽  
K. Willeke ◽  
S. Grinshpun ◽  
T. Reponen ◽  
S. Trakumas ◽  
...  

2002 ◽  
Author(s):  
I. Agranovski ◽  
V. Agranovski ◽  
S. Grinshpun ◽  
K. Willeke ◽  
T. Reponen

2001 ◽  
Author(s):  
G. Mainelis ◽  
R. Gorny ◽  
K. Willeke ◽  
S. Grinshpun ◽  
T. Reponen ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Cheng Wen ◽  
Yuanyuan Kong ◽  
Jian Zhao ◽  
Yang Li ◽  
Ya Shen ◽  
...  

Abstract Background This study aimed to compare the use of photon-initiated photoacoustic streaming (PIPS) and conventional needle irrigation (CNI) in conjunction with different concentrations of sodium hypochlorite (NaOCl) to remove Enterococcus faecalis (E. faecalis) suspended bacteria and biofilms from root canal systems with different diameters or tapers. Methods Artificial root canal samples (n = 480) were randomly divided into three groups (n = 160/group). The canals were prepared to fit file sizes #10/.02, #25/.02, or #25/.06. The size #10/.02 group was incubated for seven days. The size #25/.02 or #25/.06 group was incubated for 2 days. A stable biological model of E. faecalis infection was established. The root canals were washed with distilled water or with 1%, 2%, or 5.25% NaOCl combined with CNI or PIPS. Bacterial suspensions and biofilms were assessed using an ATP assay kit and fluorescence microscopy. Image-Pro Plus was used to analyse the average fluorescence intensity to determine the most suitable root canal irrigation solution. Results In the CNI and PIPS groups, the ATP value of the 5.25% NaOCl subgroup was the lowest, followed by that of the 2% and 1% NaOCl subgroups. The ATP value of the distilled water subgroup was the highest (P < 0.05). When the root canal taper was 0.02, the ATP value of the #10/.02 + PIPS group was significantly lower than that of the #25/.02 + CNI group (P < 0.05). The average fluorescence intensity of the #10/.02 + PIPS group was lower than that of the #25/.02 + CNI group (P < 0.05). When the apical diameter was #25, the ATP value of the 0.02 taper in the PIPS group was lower than that of the 0.06 taper in the CNI group (P < 0.05), and the average fluorescence intensity of the 0.02 taper + PIPS group was lower than that of the 0.06 taper + CNI group (P < 0.05). PIPS combined with 2% and 5.25% NaOCl effectively improved the long-term antibacterial effect after irrigation and re-culture for 6 h. Conclusions Compared with CNI, PIPS has greater ability to remove bacteria in root canals with a small preparation diameter and a small taper. PIPS with 2% and 5.25% NaOCl exhibited superior antibacterial and bacteriostatic effects.


Author(s):  
Jinbiao Ma ◽  
Manman Du ◽  
Can Wang ◽  
Xinwu Xie ◽  
Hao Wang ◽  
...  

2021 ◽  
Vol 6 (1) ◽  
pp. 53-68
Author(s):  
Chiradeep Basu ◽  
Subarna Bhattacharyya ◽  
Anirban Chaudhuri ◽  
Shaheen Akhtar ◽  
Akash Chatterjee ◽  
...  

Damaging factors such as airborne microorganisms, relative humidity, ventilation, temperature and air pollutants are the major concerns of the tropical climate of Kolkata, India where our study site, 172-year-old St. Paul’s Cathedral is located. In this context, the aim was to develop an equation to assess the management priority and which factors would be more responsible for potentially damaging the heritage building. The temperature varied from 28°C to 31°C, relative humidity was recorded 72% over a period of 14 days in the prayer hall whereas almost constant temperature (27°C) and relative humidity (55%) were recorded in crypt. Air movement was recorded 0.5–3 m s−1 in both crypt and prayer hall. Sulphur dioxide and oxide of nitrogen concentration were lower than the standard mentioned by the Central Pollution Control Board, India. The fungal load was lower inside the crypt (237 CFU m−3) than in the prayer hall (793 CFU m−3). Calculated potential damage for prayer hall and crypt was found to be 48.75% and 37.08%, respectively. Results revealed that microbial load and relative humidity were the potent factors for damage to the building. Continuous air movement, that is, ventilation and building design here played significant roles. The Heritage Conservation Committee can use the data for better management.


1995 ◽  
Author(s):  
Stephen J. Lockett ◽  
Curtis T. Thompson ◽  
James C. Mullikin ◽  
Damir Sudar ◽  
R. Khavari ◽  
...  

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