The measurement and control of high-risk host cell proteins for polysorbate degradation in biologics formulation

Non-ionic surfactant polysorbates (PS), including PS-80 and PS-20, are commonly used in the formulation of biotherapeutic products for both preventing surface adsorption and acting as stabilizer against protein aggregation. Trace levels of residual host cell proteins (HCPs) with lipase or esterase enzymatic activity have been shown to degrade polysorbates in biologics formulation. The measurement and control of these low-abundance, high-risk HCPs for polysorbate degradation is an industry-wide challenge to achieve desired shelf-life of biopharmaceuticals in liquid formulation, especially for high-concentration formulation product development. Here, we reviewed the challenges, recent advances and future opportunities of analytical method development, risk assessment and control strategies for polysorbate degradation during formulation development with a focus on enzymatic degradation. Continued efforts to advance our understanding of polysorbate degradation in biologics formulation will help develop high-quality medicines for patients.

Modeling and analysis of responsive space launch system based on timed colored Petri NeT

Responsive space launch is currently developing rapidly and has application cases in many fields, with advantages such as fast response speed and flexible networking. Aiming at the efficiency evaluation of responsive space launch system, this article first summarizes the typical process of fast space launch, and then gives a hierarchical model of a space fast launch system based on timed colored Petri nets, including top-level model, demand model, command decision model, launch Model, measurement and control model, and finally use CPN Tools to simulate and verify the model, analyze the basic characteristics of the model and the performance of the system, and provide data support and theoretical basis for the optimization of the fast space launch system.

Method Study on Improving Teaching Efficiency of Compulsory Courses --Taking Laser Principle and Application as an Example

At present, students major in photo electricity information science and engineering, measurement and control technology and instrumentation of colleges and universities are not interested in the compulsory courses which require higher theoretical basis. And teaching efficiency of such courses is bad. Aiming at the issue, we put forward some important measures on how to motivate students’ interest and improve the teaching efficiency of compulsory courses of such majors with a combination of course: Laser Principle and Application. From the aspect of teachers, a teacher shall accumulate rich application examples in various fields and prepare lessons efficiently. It is a precondition for improving compulsory course teaching efficiency. It is also effective guarantee to carry out vivid classroom teaching with a combination of cutting-edge technological development. Besides, it is fundamental guarantee for a teacher to bring in diversification means in the big data era and adopt advanced teaching methods. From the aspect of students, rich learning interest and positive learning attitude are the fundamental power to improve compulsory course teaching efficiency. In addition, necessary engineering practice ability cultivation is the fundamental goal.

Helicopter Training Simulator Measurement and Control System Based on Computer Simulation Technology

With the development of computer software technology and simulation technology, simulation training has been widely used. Helicopter training simulation has become an important part of helicopter training, and its status is becoming more and more important. On the one hand, the air mechanic is responsible for the maintenance and organization of the helicopter during the flight, and on the other hand is responsible for the starting and stopping of the helicopter engine, the operation of related equipment, and the handling of emergencies during the flight. The simulation training has the characteristics of high training efficiency, convenient maintenance and low training cost. It will become an important means for helicopter air mechanics to troubleshoot and deal with special situations. This paper aims to study the helicopter training simulator measurement and control system based on computer software technology. Based on the analysis of the advantages of the simulator instead of the actual installation for training, the helicopter training simulator function and the simulator subsystem, the actual installation simulation instrument and signal are designed. The indicator light, the graphic instrument and the control signal are arranged in the circuit, and then the simulation experiment is carried out on the design. The experimental results show that the design can more accurately simulate the indication of the antenna elevation angle table, which meets the requirements of this article.

Towards temperature controlled retinal laser treatment with a single laser at 10 kHz repetition rate

Laser photocoagulation is one of the most frequently used treatment approaches in ophthalmology for a variety of retinal diseases. Depending on indication, treatment intensity varies from application of specific micro injuries down to gentle temperature increases without inducing cell damage. Especially for the latter, proper energy dosing is still a challenging issue, which mostly relies on the physician’s experience. Pulsed laser photoacoustic temperature measurement has already proven its ability for automated irradiation control during laser treatment but suffers from a comparatively high instrumental effort due to combination with a conventional continuous wave treatment laser. In this paper, a simplified setup with a single pulsed laser at 10 kHz repetition rate is presented. The setup combines the instrumentation for treatment as well as temperature measurement and control in a single device. In order to compare the solely pulsed heating with continuous wave (cw) tissue heating, pulse energies of 4 µJ were applied with a repetition rate of 1 kHz to probe the temperature rise, respectively. With the same average laser power of 60 mW an almost identical temporal temperature course was retrieved in both irradiation modes as expected. The ability to reach and maintain a chosen aim temperature of 41 °C is demonstrated by means of model predictive control (MPC) and extended Kalman filtering at a the measurement rate of 250 Hz with an accuracy of less than ±0.1 °C. A major advantage of optimization-based control techniques like MPC is their capability of rigorously ensuring constraints, e.g., temperature limits, and thus, realizing a more reliable and secure temperature control during retinal laser irradiation.

Automatic Optical Measurement and Control of Benzene and Benzenoids in Natural Gas Pipelines

The presence of benzene and similar aromatic compounds in civil environments is due to anthropic actions but also to natural sources. Natural gas consists of a gas mixture where benzene and related compounds are usually presents. Thus, the detection of these compounds in natural gas pipelines is of the utmost importance as well as the control of the concentration level, which must remain below the limits consented by law. In this regard, it is of striking interest to engineer devices able to detect these compounds by automatic and continuous remote control. Here, we discuss the application of an optical device designed for the measurement of sulfured odorizing agents in natural gas pipelines aiming at the detection and the measurement of benzene, toluene, and xylenes (BTX) in the same contexts. The instrument consists of a customized UV spectrophotometer connected to an automatic control system able to provide in-field detections of BTX through a continuous and remote check of the gaseous mixture. Relatively to benzene, the instrument is characterized by values of LOD (level of detection) and LOQ (level of quantification) equal to 0.55 and 1.84 mg/Sm3, respectively. Similar limits are found for toluene and xylenes (LOD of 0.81, 1.05, 1.41, and 1.00 mg/Sm3 for toluene, meta-, ortho-, and para-xylene, respectively).

The Design of an IEEE 1588 End-to-End Transparent Ethernet Switch

<p>In measurement and control systems there is often a need to synchronise distributed clocks. Traditionally, synchronisation has been achieved using a dedicated medium to convey time information, typically using the IRIG-B serial protocol. The precision time protocol (IEEE 1588) has been designed as an improvement to current methods of synchronisation within a distributed network of devices. IEEE 1588 is a message based protocol that can be implemented across packet based networks including, but not limited to, Ethernet. Standard Ethernet switches introduce a variable delay to packets that inhibits path delay measurements. Transparent switches have been introduced to measure and adjust for packet delay, thus removing the negative effects that these variations cause. This thesis describes the hardware and firmware design of an IEEE 1588 transparent end-to-end Ethernet switch for Tekron International Ltd based in Lower Hutt, New Zealand. This switch has the ability to monitor all Ethernet traffic, identify IEEE 1588 timing packets, measure the delay that these packets experience while passing through the switch, and account for this delay by adjusting a time-interval field of the packet as it is leaving the switch. This process takes place at the operational speed of the port, and without introducing significant delay. Time-interval measurements can be made using a high-precision timestamp unit with a resolution of 1 ns. The total jitter introduced by this measurement process is just 4.5 ns through a single switch.</p>

The Design of an IEEE 1588 End-to-End Transparent Ethernet Switch

<p>In measurement and control systems there is often a need to synchronise distributed clocks. Traditionally, synchronisation has been achieved using a dedicated medium to convey time information, typically using the IRIG-B serial protocol. The precision time protocol (IEEE 1588) has been designed as an improvement to current methods of synchronisation within a distributed network of devices. IEEE 1588 is a message based protocol that can be implemented across packet based networks including, but not limited to, Ethernet. Standard Ethernet switches introduce a variable delay to packets that inhibits path delay measurements. Transparent switches have been introduced to measure and adjust for packet delay, thus removing the negative effects that these variations cause. This thesis describes the hardware and firmware design of an IEEE 1588 transparent end-to-end Ethernet switch for Tekron International Ltd based in Lower Hutt, New Zealand. This switch has the ability to monitor all Ethernet traffic, identify IEEE 1588 timing packets, measure the delay that these packets experience while passing through the switch, and account for this delay by adjusting a time-interval field of the packet as it is leaving the switch. This process takes place at the operational speed of the port, and without introducing significant delay. Time-interval measurements can be made using a high-precision timestamp unit with a resolution of 1 ns. The total jitter introduced by this measurement process is just 4.5 ns through a single switch.</p>