Adult Stem Cell Survival Strategies

Spermatogonial stem-cell survival after irradiation injury has been studied in rodents by histological counts of surviving cells. Many studies, including previous work from our laboratory, show that the spermatogonial population demonstrates a heterogeneous response to irradiation. The spermatogonia increase in radio-sensitivity as differentiation proceeds through the sequence As - Apr - A1 - A2 - A3 - A4 - In - B. The stem (As) cell is the most resistant and the B cell is the most sensitive. The purpose of this work is to investigate the response of spermatogonial cell to low doses (less than 10 0 rads) of helium particle irradiation.

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Faculty Opinions recommendation of Polycomb group genes are required for neural stem cell survival in postembryonic neurogenesis of Drosophila.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1087752.540665 ◽

2007 ◽

Author(s):

Leonie Ringrose

Keyword(s):

Stem Cell ◽

Cell Survival ◽

Neural Stem Cell ◽

Polycomb Group ◽

Polycomb Group Genes ◽

Stem Cell Survival

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Faculty Opinions recommendation of Fabrication and characterization of prosurvival growth factor releasing, anisotropic scaffolds for enhanced mesenchymal stem cell survival/growth and orientation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.3435970.3129070 ◽

2010 ◽

Author(s):

Dietmar Werner Hutmacher ◽

Maria Woodruff

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Mesenchymal Stem Cell ◽

Cell Survival ◽

Stem Cell Survival ◽

Fabrication And Characterization

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The enhancement of neural stem cell survival and growth by coculturing with expanded sertoli cells in vitro

Biotechnology Progress ◽

10.1002/btpr.720 ◽

2011 ◽

Vol 28 (1) ◽

pp. 196-205 ◽

Cited By ~ 6

Author(s):

Bingyang Shi ◽

Lei Deng ◽

Xiaolin Shi ◽

Sheng Dai ◽

Hu Zhang ◽

...

Keyword(s):

Stem Cell ◽

Cell Survival ◽

Neural Stem Cell ◽

Sertoli Cells ◽

Stem Cell Survival ◽

Survival And Growth

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Prosurvival and antiapoptotic effects of PGE2in radiation injury are mediated by EP2 receptor in intestine

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00240.2002 ◽

2003 ◽

Vol 284 (3) ◽

pp. G490-G498 ◽

Cited By ~ 52

Author(s):

Courtney W. Houchen ◽

Mark A. Sturmoski ◽

Shrikant Anant ◽

Richard M. Breyer ◽

William F. Stenson

Keyword(s):

Stem Cell ◽

Cell Survival ◽

Cross Section ◽

Radiation Injury ◽

Biological Activities ◽

Receptor Expression ◽

Apoptotic Cells ◽

Wild Type ◽

Γ Irradiation ◽

Stem Cell Survival

The biological activities of PGE2 are mediated through EP receptors (EP1–EP4), plasma membrane G protein-coupled receptors that differ in ligand binding and signal-transduction pathways. We investigated gastrointestinal EP2 receptor expression in adult mice before and after radiation injury and evaluated intestinal stem cell survival and crypt epithelial apoptosis after radiation injury in EP2 null mice. EP2 was expressed throughout the gut. Intestinal EP2 mRNA increased fivefold after γ-irradiation. Crypt survival was diminished in EP2 −/− mice (4.06 crypts/cross section) compared with wild-type littermates (8.15 crypts/cross section). Radiation-induced apoptosis was significantly increased in EP2 −/− mice compared with wild-type littermates. Apoptosis was 1.6-fold higher in EP2 −/− mice (5.9 apoptotic cells/crypt) than in wild-type mice (3.5 apoptotic cells/crypt). The EP2receptor is expressed in mouse gastrointestinal epithelial cells and is upregulated following radiation injury. The effects of PGE2on both crypt epithelial apoptosis and intestinal crypt stem cell survival are mediated through the EP2 receptor.

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