A preliminary study on inhibitory effect of selenium against Penicillium expansum and Botrytis cinerea

Author(s):  
Zhilin Wu ◽  
Qian Wang ◽  
Miao Li ◽  
Linxi Yuan ◽  
Ying Liu
2010 ◽  
Vol 40 (No. 4) ◽  
pp. 128-134 ◽  
Author(s):  
H. Bryk ◽  
B. Dyki ◽  
P. Sobiczewski

The influence of antagonistic Pseudomonas spp. on the development of Botrytis cinerea and Penicillium expansum was studied in liquid cultures. Two strains of Pseudomonas spp. (B194 and B224), originally isolated from apple leaves and fruits, respectively, inhibited spore germination and germ tube elongation of B. cinerea or P. expansum. The inhibitory effect depended on the concentration of bacteria in the cultures. After a prolonged time of incubation (48–76 h) lysis and fragmentation of hyphae of both fungi was observed. In some cases the hyphae of B. cinerea developed abnormally if the bacterial strains were present – the hyphal tips were swollen and ball-shaped spore-like structures aggregated in chains were formed.


2016 ◽  
Vol 12 (33) ◽  
pp. 112
Author(s):  
Hakim Alilou ◽  
Bouchaib Bencharki ◽  
Mohammed Akssira ◽  
Lalla Mina Idrissi Hassani ◽  
Noureddine Barka

Two sesquiterpenes of Asteriscus graveolens subsp. odorus, have been extracted, separated, purified and identified by NMR and IR: the naupliolide and asteriscunolide 3c. The valuation of these compounds is part of the search for a preventive approach of integrated control to reduce significantly the use of pesticides and especially the fungicide ones. The results revealed a strong inhibitory effect on naupliolide against Botrytis cinerea, Penicillium digitatum and Penicillium expansum dice concentration of 200 ppm. The asteriscunolide 3c showed a remarkable effect against Botrytis cinerea from the concentration of 150 ppm.


2015 ◽  
Vol 181 ◽  
pp. 121-128 ◽  
Author(s):  
E. Abouraïcha ◽  
Z. El Alaoui-Talibi ◽  
R. El Boutachfaiti ◽  
E. Petit ◽  
B. Courtois ◽  
...  

2008 ◽  
Vol 3 (5) ◽  
pp. 644-650 ◽  
Author(s):  
Juan Calvo ◽  
Viviana Calvente ◽  
María E. Orellano ◽  
Delia Benuzzi ◽  
Maria I. Sanz

2019 ◽  
Vol 167 (7-8) ◽  
pp. 470-478 ◽  
Author(s):  
Anwei Luo ◽  
Junqing Bai ◽  
Rui Li ◽  
Yimeng Fang ◽  
Lin Li ◽  
...  

2019 ◽  
Vol 67 (3) ◽  
pp. 109-118
Author(s):  
Lidia Kołodziejczyk ◽  
Kinga Mazurkiewicz-Zapałowicz ◽  
Magdalena Twarużek ◽  
Jan Grajewski ◽  
Łukasz Łopusiewicz ◽  
...  

The aim of the study was to evaluate the potential use of selected species of soil fungi (Fusarium oxysporum, F. sulphureum, F. verticillioides, and Penicillium expansum) for the bioregulation of the dispersive stages of a parasitic nematode – the large roundworm of pig (Ascaris suum). Experimental cultures containing A. suum eggs with soil fungi and control cultures without fungi were incubated at 26°C for 28 days. Microscopic observations of the developmental stages of the A. suum eggs (zygote, 2-8 blastomeres, morula/blastula, gastrula, and larva) were performed at 7, 14, 21, and 28 days. The API-ZYM test was used to semi-quantitatively determine the activity of 19 hydrolytic fungal enzymes. The cytotoxicity of the fungi was determined with a tetrazole salt MTT assay. Microscopic observations of A. suum eggs incubated in the presence of fungi up to day 28 did not show any signs of destruction to egg shells and/or penetration of the fungi into the eggs. The ovistatic effect of all tested fungi (F. sulphureum, P. expansum, F. verticillioides, and F. oxysporum; p<0.05) was seen only on the 7th day of incubation, whereas on the 14th day, only F. verticillioides and F. oxysporum showed an inhibitory effect on the embryogenesis of A. suum, and by the 28th day, only P. expansum. The API-ZYM test showed differences in the hydrolytic activity of the tested strains, while the MTT assay showed the high cytotoxicity of F. sulphureum, the moderate cytotoxicity of F. verticillioides and P. expansum, and the low cytotoxicity of F. oxysporum. Among the fungal strains studied, F. sulphureum showed the highest ovistatic effect, which may be related to its enzymatic activity and cytotoxicity.


2015 ◽  
Vol 4 (3) ◽  
pp. 128 ◽  
Author(s):  
Naresh Shahi ◽  
ByungJin Min ◽  
Eunice A. Bonsi

<p>This study was carried out to determine the effect of natural antimicrobial washing solutions against microbial growths on fresh produce specifically strawberries. Selected washing solutions used for strawberry washing, and treatments were sterile water (control), white vinegar (VI), crude lemon juice extract (LE), VI+Origanum oil (VIO), LE+Origanum oil (LEO), and VI+LE+Origanum oil (VILEO). From the preliminary study of antimicrobial activity of washing solutions in aqueous model system tested at 2, 5, 10, 15, 20 and 25 min against <em>S.</em> Typhimurium, washing time was determined as 5 min to be used for this study. After the washing, strawberries were stored at 4 °C for 5 days. Results showed that all natural washing solutions exhibited inhibitory effect against total aerobic bacteria, yeast and mold. On day 5, compared to the control, all washing solutions significantly reduced <em>S.</em> Typhimurium by 2.7 Log CFU/g (P&lt;0.05). Color results showed that samples color were slightly changed by washing with VIO and VILEO. However, there was no significant difference in total color change on strawberries compared to the control (P&gt;0.05). Based on the results, it is indicated that the combination of vinegar with crude lemon juice extract and essential oil might be suitable as natural sanitizer for decontamination of fresh produce.</p>


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