Biodiversity and Conservation of Plant Genetic Resources (IN-SITU and EX-SITU)

2018 ◽  
pp. 79-106
Author(s):  
Sheel Yadav ◽  
Chet Ram ◽  
DhammaPrakash Pandhari Wankhede
Author(s):  
Jane Muthoni ◽  
Hussein Shimelis ◽  
Rob Melis

Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.


CORD ◽  
2005 ◽  
Vol 21 (02) ◽  
pp. 34
Author(s):  
Pons Batugal

The International Coconut Genetic Resources Network (COGENT) is a global research network organized by the International Plant Genetic Resources Institute (IPGRI) in 1992 with support from member countries, the Consultative Group on International Agricultural Research (CGIAR), partner institutions, donor agencies, and by regional and international development organizations.                           In the last 12 years, COGENT has been fully operational with 38 member coconut producing countries in five regions (South Asia; Southeast and East Asia; South Pacific; Africa and the Indian Ocean; and Latin America and the Caribbean). It has successfully developed and disseminated to coconut breeders and curators worldwide the International Coconut Genetic Resources Database (CGRD). The CGRD contains characterization data and some pictures of 1,416 accessions which are conserved by national programmes in 28 sites in 23 countries. To further secure conserved germplasm, a COGENT multi-site International Coconut Genebank has been established to conserve 200 important accessions in each region. Coconut varieties with multi-purpose uses are being identified, documented and promoted. The performance of promising 38 high-yielding hybrids are being evaluated in a multilocation  trial involving four African and three Latin America/Caribbean countries to identify suitable varieties and hybrids for resource-poor farmers. Farmers’ varietal preferences in 15 countries are being evaluated. Diversity-linked income-generating activities are being used as a strategy to promote in situ and on-farm conservation and germplasm utilization have been initiated in 15 countries. Protocols for in vitro embryo culture, cryopreservation, morphometric and molecular marker-based methods for locating and characterizing diversity; pest risk assessment and germplasm health management are being developed, tested and upgraded. Strategies and techniques for farmer participatory research, collecting, characterization and ex situ and in situ conservation are being refined.   To strengthen the coconut research capability of COGENT member countries, the COGENT Secretariat and IPGRI have organized 39 country need assessment missions  and conducted 41 workshops and meetings involving 994 coconut researchers to share information and technologies, discuss issues and common problems and opportunities and how to address them; conducted 40 training courses involving 765 participants from 41 countries; supported 274  research and training/capacity building activities  in 30 countries; and led the establishment of the Global Coconut Research for Development Programme (PROCORD). IPGRI and COGENT's current priority involves the further promotion of more effective conservation and use of coconut genetic resources, both regionally and globally.


2017 ◽  
Vol 40 (1) ◽  
pp. 93-101
Author(s):  
Daymara Rodríguez-Alfonso ◽  
Miriam Isidrón-Pérez ◽  
Dubiel Alfonso-González ◽  
María J. Grajal-Martín ◽  
José I. Hormaza-Uroz ◽  
...  

Conservation of plant genetic resources (PGR) is essential to preserve diversity and to provide genes for plant breeding. This paper assesses the current status of pineapple PGR diversity in Cuba and actions are proposed to minimize the loss of diversity. In situ diversity was evaluated through field trips to different locations across the country, evidence was found that pineapple germplasm diversity is low. Only three (Spanish, Cayenne and Pernambuco) out of the five horticultural groups of this crop are presently planted at Cuba. Red Spanish is the predominant cultivar, and White Pineapple is an endangered one. The highest diversity was found at the Eastern region, where it was possible to find at least two different cultivars from each of these three groups. The ex situ pineapple collection contains 56 accessions, 45 % belong to the Spanish group, 20 % to Cayenne and 14 % to Pernambuco, while the rest are hybrids, improved cultivars and other related species. Threats of diversity loss were identified by the Research-Action-Participation method. Farmers and experts agreed that growing of the most common cultivars is being abandoned and consequently, there is high risk of loss of in situ diversity. Results document the low diversity of pineapple genetic resources in the country and the need to use in situ and ex situ conservation approaches as complementary strategies for germplasm preservation for future generations.


2010 ◽  
Vol 11 (1) ◽  
pp. 85 ◽  
Author(s):  
Rubén Alfredo Valencia ◽  
R, Mario Lobo A. ◽  
Gustavo Adolfo Ligarreto M.

<p>Colombia es reconocida en el mundo por su megadiversidad en fauna, flora y microorganismos, atribuida a su gran complejidad ecosistémica y a procesos evolutivos de los Andes, la Orinoquia, Amazonia y de sus costas Pacífica y Caribe, en los que se encuentran páramos, selvas tropicales, humedales, llanuras y desiertos, entre otros. Con una superficie continental de alrededor del 0,77% del área terrestre del mundo, alberga aproximadamente el 10% de las especies vegetales y animales conocidas. Estos recursos genéticos tienen un valor estratégico importante para el país, y sus inventarios son una herramienta fundamental para el análisis del estado actual y potencial de ellos y para la toma de decisiones sobre medidas de conservación y renovación. En este ámbito, el Gobierno colombiano facilitó la conformación del Sistema de Bancos de Germoplasma de la Nación para la Alimentación y la Agricultura, el cual figura en cabeza del ICA y es manejado por Corpoica por medio de un convenio de Cooperación Técnica y Científica, suscrito con el ICA. Del total de accesiones vegetales que posee Colombia, mantenidas en condiciones ex situ, el 70% se maneja en Corpoica. El 30% restante corresponde a bancos activos. En general, en Colombia existen bancos de germoplasma donde se conservan las especies en la modalidad ex situ, lo que complementa procesos de mantenimiento in situ, que incluyen materiales en fincas de los productores.</p><p><strong><br /></strong></p><p><strong>State of Research of Plant Genetic Resources in Colombia: Germplasm Banks System</strong></p><p>Colombia is recognized worldwide for its megadiversity, which includes fauna, flora and microorganisms. The above is attributed to its highly ecosystemic complexity, derived from evolutionary processes in the Andes, the Orinoco, the Amazon and its Pacific and Caribbean coasts; regions where are located highlands, tropical jungles, wetlands, plains and deserts, among others. With about 0.77%, of the world’s land area, the country holds approximately 10% of the plant and animal species known around the world. These genetic resources hold an important strategic value for the country, and their inventories are a fundamental tool for the analysis of their current option and use values, as well as, for taking actions related to their conservation, renewal and utilization. In this context, the Colombian government promoted the establishment of a National Germplasm Bank System for Food and Agriculture, which comprises vegetal, animal and microorganism species, The System is administrated by ICA and managed by Corpoica, through a Technical and Scientific Cooperation Agreement subscribed by the last two entities. From all the plant species accessions, held under ex situ conditions at Colombia, 70% of those are included in the above System. The remaining 30% correspond, mainly to species, held under the criteria of active banks. From the above and, in relation to plant genetic resources, Colombia has an ex situ National Germplasm Bank System, for Food and Agriculture, which complements in situ conservation processes done in farm by local producers.</p>


2020 ◽  
Vol 15 (2) ◽  
pp. 107-126
Author(s):  
M Sankaran ◽  
M R Dinesh

India is one of the 12 mega biodiversity centres with 2 biodiversity hotspots which are the reservoirs of plant genetic resources. India stands at 7thplace in the global agricultural biodiversity status. Among fruit and nut crops, there are about 117 cultivated species with 175 wild relatives of which only 25 species have been domesticated. Genetic resources conservation of fruit trees is intricate and complex as they are belonging to various genera and species which require specific climate. Hence, in situ and ex situ conservation can go simultaneously. The western ghat and North eastern India are centers of diversity for several important native fruits including Mango, Jackfruit and Citrus. Apart from the major fruit crops, India is home to several underutilized fruit crops. However, due to increased pressure on land use several of the wild types, which are a great source of genes governing useful traits, are disappearing. Thus, there is an urgent need to conserve them in both in situ and ex situ conditions. The genetic diversity and modes of conservation of tropical fruits are discussed in this paper.


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  

2012 ◽  
Vol 10 (3) ◽  
pp. 242-253 ◽  
Author(s):  
Holly Vincent ◽  
Roland von Bothmer ◽  
Helmut Knüpffer ◽  
Ahmed Amri ◽  
Jan Konopka ◽  
...  

To facilitate the updating ofin situandex situconservation strategies for wild taxa of the genusHordeumL., a combined ecogeographic survey and gap analysis was undertaken. The analysis was based on the Global Inventory of Barley Plant Genetic Resources held by ICARDA plus additional datasets, resulting in a database containing 17,131 wildHordeumaccessions. The analysis concluded that a genetic reserve should be established in the Mendoza Province of Argentina, as this is the most species-rich area globally forHordeum. A network of reserves should also be set up across the Fertile Crescent in Israel, Palestine, Syria, Jordan, Lebanon and Turkey to provide effective conservation within the centres of diversity for gene pools 1B (Hordeum vulgaresubsp.spontaneum(C. Koch) Thell.) and 2 (Hordeum bulbosumL.). The majority of the species were deemed under-collected, so further collecting missions are required worldwide where possible. Althoughex situandin situconservation strategies have been developed, there needs to be further investigation into the ecological environments thatHordeumspecies occupy to ensure that any adaptive traits expressed are fully conserved. Additionally, studies are required to characterize existing collections and test the viability of rare species accessions held in genebanks to determine whether furtherex situcollections are required alongside the proposedin situconservation.


Author(s):  
Roland Bourdeix ◽  
Steve Adkins ◽  
Vincent Johnson ◽  
Lalith Perera ◽  
Sisunandar

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 221-227
Author(s):  
Jelena Aleksic ◽  
Sasa Orlovic

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.


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