scholarly journals ESTROGEN EFFECTS IN NEURAL CELLS IN VITRO: MORPHOLOGIC CHARACTERIZATION OF ESTROGEN RECEPTOR (ER)-TRANSFECTED PC12 CELLS

1993 ◽  
Vol 33 ◽  
pp. S32-S32
Author(s):  
R H Luslig ◽  
P W Baas
Keyword(s):  
Estrogen Receptor ◽  
Pc12 Cells ◽  
Neural Cells ◽  
Estrogen Effects

In vivo characterization of estrogen receptor modulators with reduced genomic versus nongenomic activity in vitro

2008 ◽  
Vol 111 (1-2) ◽  
pp. 95-100 ◽  
Author(s):  
Christiane Otto ◽  
Iris Fuchs ◽  
Helga Altmann ◽  
Mario Klewer ◽  
Gilda Schwarz ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Modulators ◽  
In Vivo Characterization ◽  
Receptor Modulators

Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors

2010 ◽  
Vol 19 (4) ◽  
pp. 471-486 ◽  
Author(s):  
Nataliya Kozubenko ◽  
Karolina Turnovcova ◽  
Miroslava Kapcalova ◽  
Olena Butenko ◽  
Miroslava Anderova ◽  
...  
Keyword(s):  
Embryonic Stem ◽  
Cellular Heterogeneity ◽  
Neural Cells ◽  
Lineage Specification ◽  
Neural Precursors ◽  
Selection Strategies

During the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating NPs from the CCTL14 line of hESCs. These NPs could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and lineage specification. To analyze the population of the derived NPs, we used fluorescence-activated cell sorting (FACS) and characterized the expression of several pluripotent and neural markers, such as Nanog, SSEA-4, SSEA-1, TRA-1-60, CD24, CD133, CD56 (NCAM), β-III-tubulin, NF70, nestin, CD271 (NGFR), CD29, CD73, and CD105 during long-term propagation. The analyzed cells were used for transplantation into the injured rodent brain; the tumorigenicity of the transplanted cells was apparently eliminated following long-term culture. These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies.

[77Br]-17-α-Bromoethynylestradiol: In vivo and In vitro characterization of an estrogen receptor radiotracer

1982 ◽  
Vol 9 (4) ◽  
pp. 245-250 ◽  
Author(s):  
R.E. Gibson ◽  
W.C. Eckelman ◽  
B. Francis ◽  
H.A. O'Brien ◽  
J.K. Mazaitis ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
In Vitro Characterization

Cloning and In Vitro Expression and Characterization of the Androgen Receptor and Isolation of Estrogen Receptor α from the Fathead Minnow (Pimephales promelas)

2004 ◽  
Vol 38 (23) ◽  
pp. 6314-6321 ◽  
Author(s):  
Vickie S. Wilson ◽  
Mary C. Cardon ◽  
Joseph Thornton ◽  
Joseph J. Korte ◽  
Gerald T. Ankley ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Androgen Receptor ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Estrogen Receptor Α ◽  
In Vitro Expression

scholarly journals Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine

2014 ◽  
Vol 34 (2) ◽  
Author(s):  
Tingting Sun ◽  
Chao Yu ◽  
Yuhua Gao ◽  
Chenqiong Zhao ◽  
Jinlian Hua ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Types ◽  
Neural Cells ◽  
Biological Characterization ◽  
Multipotent Stem Cells ◽  
Reverse Transcription Pcr ◽  
Lineage Differentiation

The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.

scholarly journals An in vitro model for the effects of estrogen on neurons employing estrogen receptor-transfected PC12 cells

1994 ◽  
Vol 14 (6) ◽  
pp. 3945-3957 ◽  
Author(s):  
RH Lustig ◽  
P Hua ◽  
W Yu ◽  
FJ Ahmad ◽  
PW Baas
Keyword(s):  
Estrogen Receptor ◽  
Pc12 Cells ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Molecular Characterization of a Nondemyelinating Variant of Daniel’s Strain of Theiler’s Virus Isolated from a Persistently Infected Glioma Cell Line

1998 ◽  
Vol 72 (2) ◽  
pp. 1262-1269 ◽  
Author(s):  
Xiaoqi Lin ◽  
Shigeru Sato ◽  
Amy K. Patick ◽  
Larry R. Pease ◽  
Raymond P. Roos ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Point Mutations ◽  
Glioma Cell Line ◽  
Neural Cells ◽  
Immune Recognition ◽  
Theiler's Virus

ABSTRACT Wild-type Daniel’s strain of Theiler’s virus (wt-DA) induces a chronic demyelination in susceptible mice which is similar to multiple sclerosis. A variant of wt-DA (designated DA-P12) generated during the 12th passage of persistent infection of a G26-20 glioma cell line failed to persist and induce demyelination in SJL/J mice. To identify the determinants responsible for this change in phenotype, we sequenced the capsid coding sequence (nucleotides [nt] 2991 to 3994) and found three mutations in VP1: residues 99 (Gly to Ser), 100 (Gly to Asp), and 103 (Asn to Lys). To study the role of these mutations in neurovirulence and demyelination, we prepared a recombinant virus, DAP-1C-2A/DA, with replacement of wt-DA nt 2991 to 3994 with the corresponding region of DA-P12, and viruses with individual point mutations at VP1 residues 99(Ser), 100(Asp), and 103(Lys). DAP-1C-2A/DA and viruses with a mutation at VP1 residue 99 or 100 (but not 103) completely attenuated the ability of wt-DA to induce demyelination. Failure to induce demyelination was not due to a general failure in growth, since DA-P12 and other mutant viruses lysed L-2 cells in vitro as effectively as wt-DA. The change in disease phenotype was independent of the specific B- or T-cell immune recognition because a decrease in the neurovirulence of mutant viruses was observed in neonatal mice and immune-deficient RAG1 −/− mice. This difference in neurovirulence is not the complete explanation for the failure of DA-P12 to demyelinate, since virus with a mutation at residue 103(Lys) had decreased neurovirulence but did induce demyelination. Therefore, point mutation at VP1 residue 99 or 100 altered the ability of wt-DA to demyelinate, perhaps related to a disruption in interaction between virus and receptor on certain neural cells.

scholarly journals Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Jiao Liu ◽  
Kailin Jiao ◽  
Qian Zhou ◽  
Jun Yang ◽  
Keke Yang ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Locomotor Behavior ◽  
Sirtuin 1 ◽  
Neural Cells ◽  
Zebrafish Model ◽  
Zebrafish Larvae ◽  
Stat3 Signaling ◽  
Bv2 Cells

The oxysterol 27-hydroxycholesterol (27HC) is the first identified endogenous selective estrogen receptor modulator (SERM), which like endogenous estrogen 17β-estradiol (E2) induces the proliferation of estrogen receptor- (ER-) positive breast cancer cells in vitro. However, 27HC differs from E2 in that it shows adverse effects in the nervous system. Our previous study confirmed that 27HC could induce neural senescence by activating phosphorylated signal transducer and activator of transcription, which E2 could not. The purpose of the present study is to investigate whether STAT3 acetylation was involved in 27HC-induced neural senescence. Microglia (BV2 cells) and rat pheochromocytoma cells (PC12 cells) were used in vitro to explore the effect of resveratrol (REV) on 27HC-induced neural senescence. Senescence-associated β-galactosidase (SA-β-Gal) staining was performed using an SA-β-Gal Staining Kit in cells and zebrafish larvae. Zebrafish were used in vivo to assess the effect of 27HC on locomotor behavior and aging. We found that 27HC could induce senescence in neural cells, and REV, which has been employed as a Sirtuin-1 (SIRT1) agonist, could attenuate 27HC-induced senescence by inhibiting STAT3 signaling via SIRT1. Moreover, in the zebrafish model, REV attenuated 27HC-induced locomotor behavior disorder and aging in the spinal cord of zebrafish larvae, which was also associated with the activation of SIRT1-mediated STAT3 signaling. Our findings unveiled a novel mechanism by which REV alleviates 27HC-induced senescence in neural cells and affects zebrafish locomotor behavior by activating SIRT1-mediated STAT3 signaling.

Sign in / Sign up

Export Citation Format

Share Document