scholarly journals Estrogen Receptor β Messenger Ribonucleic Acid Expression in the Forebrain of Proestrous, Pregnant, and Lactating Female Rats

Endocrinology ◽  
2003 ◽  
Vol 144 (5) ◽  
pp. 1869-1875 ◽  
Author(s):  
Béatrice Gréco ◽  
Laura S. Lubbers ◽  
Jeffrey D. Blaustein
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Paraventricular Nucleus ◽  
Supraoptic Nucleus ◽  
Preoptic Area ◽  
Female Rats ◽  
Medial Amygdala ◽  
Female Rat ◽  
Physiological Conditions ◽  
Pregnant Females

Estrogen receptor (ER)β is present in hypothalamic and limbic neurons of female rat brains, but little is known about its regulation under physiological conditions. To determine whether ERβ expression varies during physiological conditions in which sex steroid hormone profiles are significantly different, we used in situ hybridization to assess ERβ mRNA expression in the periventricular preoptic area, bed nucleus of stria terminalis, paraventricular nucleus, supraoptic nucleus, and the posterodorsal medial amygdala of female rats on proestrus, on d 22 of pregnancy, or on d 10 of lactation (L10). In the periventricular preoptic area, d-22 pregnant females had fewer ERβ-mRNA-expressing cells than did females at proestrus, but the level of ERβ mRNA expression per cell in pregnant females was higher than in the two other groups. In the paraventricular nucleus, no changes in ERβ mRNA expression were observed; whereas in the supraoptic nucleus, proestrous females had fewer ERβ-mRNA-expressing cells than L10 females. In the posterodorsal medial amygdala, proestrous females had a greater number of ERβ-mRNA-expressing cells than did L10 females. These results demonstrate that ERβ mRNA expression is differentially regulated in a brain-region-specific and temporal manner under physiological conditions and suggest that ERβ may participate in the regulation of estrogen-sensitive reproductive functions in female rats.

scholarly journals Estradiol Dose-Dependent Regulation of Membrane Estrogen Receptor-α, Metabotropic Glutamate Receptor-1a, and Their Complexes in the Arcuate Nucleus of the Hypothalamus in Female Rats

Endocrinology ◽  
2013 ◽  
Vol 154 (9) ◽  
pp. 3251-3260 ◽  
Author(s):  
Matthew Mahavongtrakul ◽  
Martha P. Kanjiya ◽  
Maribel Maciel ◽  
Shrey Kanjiya ◽  
Kevin Sinchak
Keyword(s):  
Estrogen Receptor ◽  
Glutamate Receptor ◽  
Arcuate Nucleus ◽  
Metabotropic Glutamate Receptor ◽  
Estradiol Benzoate ◽  
Estrogen Receptor Α ◽  
Female Rats ◽  
Female Rat ◽  
Metabotropic Glutamate ◽  
Dose Dependent

Sexual receptivity in the female rat is dependent on dose and duration of estradiol exposure. A 2 μg dose of estradiol benzoate (EB) primes reproductive behavior circuits without facilitating lordosis. However, 50 μg EB facilitates lordosis after 48 hours. Both EB doses activate membrane estrogen receptor-α (mERα) that complexes with and signals through metabotropic glutamate receptor-1a (mGluR1a). This mERα-mGluR1a signaling activates a multisynaptic lordosis-inhibiting circuit in the arcuate nucleus (ARH) that releases β-endorphin in the medial preoptic nucleus (MPN), activating μ-opioid receptors (MOP). MPN MOP activation is maintained, inhibiting lordosis for 48 hours by 2 μg EB, whereas 50 μg EB at 48 hours deactivates MPN MOP, facilitating lordosis. We hypothesized that 50 μg EB down-regulates ERα and mERα-mGluR1a complexes in the ARH to remove mERα-mGluR1a signaling. In experiment I, 48 hours after 2 μg or 50 μg EB, the number of ARH ERα-immunopositive cells was reduced compared with controls. In experiment II, compared with oil controls, total ARH ERα protein was decreased 48 hours after 50 μg EB, but the 2 μg dose was not. These results indicate that both EB doses reduced the total number of cells expressing ERα, but 2 μg EB may have maintained or increased ERα expressed per cell, whereas 50 μg EB appeared to reduce total ERα per cell. In experiment III, coimmunoprecipitation and Western blot revealed that total mERα and coimmunoprecipitated mERα with mGluR1a were greater 48 hours after 2 μg EB treatment vs rats receiving 50 μg EB. These results indicate 2 μg EB maintains but 50 μg EB down-regulates mERα-mGluR1a to regulate the lordosis circuit activity.

scholarly journals Effects of Prepubertal or Adult Site-Specific Knockdown of Estrogen Receptor β in the Medial Preoptic Area and Medial Amygdala on Social Behaviors in Male Mice

eNeuro ◽  
2016 ◽  
Vol 3 (2) ◽  
pp. ENEURO.0155-15.2016 ◽  
Author(s):  
Mariko Nakata ◽  
Kazuhiro Sano ◽  
Sergei Musatov ◽  
Naoko Yamaguchi ◽  
Toshiro Sakamoto ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Preoptic Area ◽  
Social Behaviors ◽  
Medial Preoptic Area ◽  
Estrogen Receptor Β ◽  
Medial Amygdala ◽  
Male Mice ◽  
Site Specific

scholarly journals Nesfatin-1 Neurons in Paraventricular and Supraoptic Nuclei of the Rat Hypothalamus Coexpress Oxytocin and Vasopressin and Are Activated by Refeeding

Endocrinology ◽  
2007 ◽  
Vol 149 (3) ◽  
pp. 1295-1301 ◽  
Author(s):  
Daisuke Kohno ◽  
Masanori Nakata ◽  
Yuko Maejima ◽  
Hiroyuki Shimizu ◽  
Udval Sedbazar ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Mrna Expression ◽  
Paraventricular Nucleus ◽  
Supraoptic Nucleus ◽  
Energy Homeostasis ◽  
Rat Hypothalamus ◽  
Hypothalamic Nuclei ◽  
Vasopressin Neurons ◽  
Fine Localization ◽  
Supraoptic Nuclei

Nesfatin-1, a newly discovered satiety molecule, is located in the hypothalamic nuclei, including the paraventricular nucleus (PVN) and supraoptic nucleus (SON). In this study, fine localization and regulation of nesfatin-1 neurons in the PVN and SON were investigated by immunohistochemistry of neuropeptides and c-Fos. In the PVN, 24% of nesfatin-1 neurons overlapped with oxytocin, 18% with vasopressin, 13% with CRH, and 12% with TRH neurons. In the SON, 35% of nesfatin-1 neurons overlapped with oxytocin and 28% with vasopressin. After a 48-h fast, refeeding for 2 h dramatically increased the number of nesfatin-1 neurons expressing c-Fos immunoreactivity by approximately 10 times in the PVN and 30 times in the SON, compared with the fasting controls. In the SON, refeeding also significantly increased the number of nesfatin-1-immunoreactive neurons and NUCB2 mRNA expression, compared with fasting. These results indicate that nesfatin-1 neurons in the PVN and SON highly overlap with oxytocin and vasopressin neurons and that they are activated markedly by refeeding. Feeding-activated nesfatin-1 neurons in the PVN and SON could play a role in the postprandial regulation of feeding behavior and energy homeostasis.

IMMUNOREACTIVE LUTEINIZING HORMONE RELEASING FACTOR IN PITUITARY STALK BLOOD FROM FEMALE RATS: SEX STEROID MODULATION OF RESPONSE TO ELECTRICAL STIMULATION OF PREOPTIC AREA OR MEDIAN EMINENCE

1976 ◽  
Vol 70 (3) ◽  
pp. 501-511 ◽  
Author(s):  
NANCY M. SHERWOOD ◽  
SHARON A. CHIAPPA ◽  
G. FINK
Keyword(s):  
Electrical Stimulation ◽  
Median Eminence ◽  
Preoptic Area ◽  
Neural Mechanism ◽  
Female Rats ◽  
Pituitary Stalk ◽  
Facilitatory Effect ◽  
Sex Steroid ◽  
Female Rat ◽  
Stimulation Of

SUMMARY The effects of sex steroid hormones on the responsiveness of the neural mechanism responsible for the secretion of LH-RF have been examined in the female rat. Responsiveness was determined at pro-oestrus by measuring the increments in immunoreactive LH-RF of pituitary stalk blood produced by electrical stimulation of the medial preoptic area or median eminence. Ovariectomy on the morning of dioestrus reduced the LH-RF response to preoptic stimulation while oestradiol benzoate (OB) or testosterone propionate (TP) administered immediately after ovariectomy significantly augmented the response. The facilitatory effect of TP was possibly due to its conversion to an aromatized derivative since 5α-dihydrotestosterone monobenzoate was ineffective. Progesterone did not facilitate preoptic responsiveness, and, when administered to animals ovariectomized at 12.00 h of pro-oestrus, reduced the LH-RF response at 18.00 h the same day. Stimulation of the median eminence produced a significantly greater increment in LH-RF than stimulation of the preoptic area. The facilitatory action of OB on the LH-RF response was less marked for median eminence compared with preoptic stimulation. The administration of ICI 46474 at 17.00 h of dioestrus did not reduce preoptic responsiveness on the morning of the next day, suggesting that this compound does not act as an 'antioestrogen' at the level of the preoptic area.

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