scholarly journals Craniopharyngiomas Express Embryonic Stem Cell Markers (SOX2, OCT4, KLF4, and SOX9) as Pituitary Stem Cells but Do Not Coexpress RET/GFRA3 Receptors

2012 ◽  
Vol 97 (1) ◽  
pp. E80-E87 ◽  
Author(s):  
Montserrat Garcia-Lavandeira ◽  
Carmen Saez ◽  
Esther Diaz-Rodriguez ◽  
Sihara Perez-Romero ◽  
Ana Senra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Human Pituitary ◽  
Cell Markers ◽  
Embryonic Stem Cell Markers

Context: Adult stem cells maintain some markers expressed by embryonic stem cells and express other specific markers depending on the organ where they reside. Recently, stem/progenitor cells in the rodent and human pituitary have been characterized as expressing GFRA2/RET, PROP1, and stem cell markers such as SOX2 and OCT4 (GPS cells). Objective: Our objective was to detect other specific markers of the pituitary stem cells and to investigate whether craniopharyngiomas (CRF), a tumor potentially derived from Rathke's pouch remnants, express similar markers as normal pituitary stem cells. Design: We conducted mRNA and Western blot studies in pituitary extracts, and immunohistochemistry and immunofluorescence on sections from normal rat and human pituitaries and 20 CRF (18 adamantinomatous and two papillary). Results: Normal pituitary GPS stem cells localized in the marginal zone (MZ) express three key embryonic stem cell markers, SOX2, OCT4, and KLF4, in addition to SOX9 and PROP1 and β-catenin overexpression. They express the RET receptor and its GFRA2 coreceptor but also express the coreceptor GFRA3 that could be detected in the MZ of paraffin pituitary sections. CRF maintain the expression of SOX2, OCT4, KLF4, SOX9, and β-catenin. However, RET and GFRA3 expression was altered in CRF. In 25% (five of 20), both RET and GFRA3 were detected but not colocalized in the same cells. The other 75% (15 of 20) lose the expression of RET, GFRA3, or both proteins simultaneously. Conclusions: Human pituitary adult stem/progenitor cells (GPS) located in the MZ are characterized by expression of embryonic stem cell markers SOX2, OCT4, and KLF4 plus the specific pituitary embryonic factor PROP1 and the RET system. Redundancy in RET coreceptor expression (GFRA2 and GFRA3) suggest an important systematic function in their physiological behavior. CRF share the stem cell markers suggesting a common origin with GPS. However, the lack of expression of the RET/GFRA system could be related to the cell mislocation and deregulated growth of CRF.

Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers

2006 ◽  
Vol 184 (3-4) ◽  
pp. 105-116 ◽  
Author(s):  
Irina Kerkis ◽  
Alexandre Kerkis ◽  
Dmitri Dozortsev ◽  
Gaëlle Chopin Stukart-Parsons ◽  
Sílvia Maria Gomes Massironi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Dental Pulp ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Isolation And Characterization ◽  
Embryonic Stem Cell Markers

scholarly journals Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Francesca Diomede ◽  
Thangavelu Soundara Rajan ◽  
Valentina Gatta ◽  
Marco D’Aurora ◽  
Ilaria Merciaro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Proliferation Assay ◽  
Cell Markers ◽  
Late Passage ◽  
Embryonic Stem Cell Markers

Background. Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties and are emerging as a potential therapeutic tool to treat diverse diseases, such as multiple sclerosis, myocardial infarction, and connective tissue damages. In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer. In vitro passages may eventually modify these embryonic marker expressions and other stemness properties, including proliferation. In the present study, we have investigated the expression of proteins involved in cell proliferation/senescence and embryonic stem cell markers during early (passage 2) and late passages (passage 15) in MSCs obtained from human gingiva, periodontal, and dental pulp tissues. Methods. Cell proliferation assay, beta galactosidase staining, immunocytochemistry, and real-time PCR techniques were applied. Results. Cell proliferation assay showed no difference between early and late passages while senescence markers p16 and p21 were considerably increased in late passage. Embryonic stem cell markers including SKIL, MEIS1, and JARID2 were differentially modulated between P2 and P15 cells. Discussion. Our results suggest that the presence of embryonic and proliferation markers even in late passage may potentially endorse the application of dental-derived MSCs in stem cell therapy-based clinical trials.

scholarly journals Detection of embryonic stem cell markers in adult human adipose tissue-derived stem cells

2011 ◽  
Vol 54 (3) ◽  
pp. 501 ◽  
Author(s):  
SarasaBharati Arumugam ◽  
OmanaA Trentz ◽  
Devi Arikketh ◽  
Vijayalakshmi Senthinathan ◽  
Barry Rosario ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Human Adipose Tissue ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Adult Human ◽  
Embryonic Stem Cell Markers

The expression of mesenchymal and embryonic stem cell markers by human limbal Stromal cells

2011 ◽  
Author(s):  
Moon Nian Lim ◽  
Umapathy Thiageswari ◽  
Othman Ainoon ◽  
P. J. N. Baharuddin ◽  
R. A. Jamal ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Stromal Cells ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Embryonic Stem Cell Markers

Expression of embryonic stem cell markers in pyogenic granuloma

2016 ◽  
Vol 43 (12) ◽  
pp. 1096-1101 ◽  
Author(s):  
Max G. Blackwell ◽  
Tinte Itinteang ◽  
Alice M. Chibnall ◽  
Paul F. Davis ◽  
Swee T. Tan
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Pyogenic Granuloma ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Embryonic Stem Cell Markers

scholarly journals Expression of embryonic stem cell markers in acute myeloid leukemia

Tumor Biology ◽  
2017 ◽  
Vol 39 (7) ◽  
pp. 101042831771662 ◽  
Author(s):  
Tiphanie Picot ◽  
Carmen Mariana Aanei ◽  
Amandine Fayard ◽  
Pascale Flandrin-Gresta ◽  
Sylvie Tondeur ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Myeloid Leukemia ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Embryonic Stem Cell Markers ◽  
Acute Myeloid

scholarly journals Increased Stem Cell Marker Expressions during the Peri-Implantation Period in the Rat Endometrium: Constructive Role of Exogenous Zinc and/or Progesterone

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Cagdas Sahin ◽  
Ozlem Yilmaz Dilsiz ◽  
Sirin Bakti Demiray ◽  
Ozgur Yeniel ◽  
Mete Ergenoglu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Marker ◽  
Control Group ◽  
Stem Cell Markers ◽  
Cell Marker ◽  
Cell Markers ◽  
Embryonic Stem Cell Markers ◽  
Implantation Period

Background. The aim of this study is to determine the effects of zinc and/or progesterone via the expression ofαvβ5 integrins and Vitronectins and embryonic stem cell markers during the peri-implantation period.Methods. Four experimental groups were organized. All subjects were mated with males of the same strain to induce pregnancy; after 5 days, zinc and/or progesterone were administered. Blood levels of zinc and progesterone were determined on the sixth day and endometrial tissues were obtained in order to evaluate the immunohistochemical expression of integrins and embryonic stem cell markers.Results. Theαvβ5 integrin and vitronectin expression increased in the zinc group compared with the control group and no difference in the progesterone group and zinc + progesterone group. Expression of Klf-4, Sox-2, and c-Myc was found to be increased in the zinc group compared to controls, while no difference was determined between the progesterone, zinc + progesterone, and control groups. Distinctively, expression of the embryonic stem cell marker Oct-4 was increased in all of the experimental groups.Conclusions. Expression ofαvβ5 integrin, vitronectin, and embryonic stem cell markers might be increased by the administration of zinc. Our results suggest that zinc could be useful in the induction of implantation rates.

scholarly journals Stem Cell and Markers

2019 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Adult Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Wide Range

Stem cells have the ability to go through various cell divisions and also maintain undifferentiated state. Stem cells are Embryonic (Pluripotent) and adult stem cells. Pluripotent stem cells give rise to all tissues such as ectoderm, mesoderm and endoderm. Embryonic stem cells isolated from inner cell mass of embryo blastocyst. Adult stem cells are also undifferentiated cells present in adult organisms and repair the tissue when damaged occurs but number in less. Adult stem cells are present in bone marrow, adipose tissue, blood and juvenile state umbilical cord and tissue of specific origin like liver, heart, intestine and neural tissue. Embryonic stem cells from blastocyst have the ethical problems and tumorogenecity. These can be identified by flow cytometry. There are wide range of stem cell markers which are useful in identifying them. Most of the pluripotent cell markers are common with tumor cell markers which throws a challenge for certainty.

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