Immunohistochemical Localization of a Human Chorionic Gonadotropin-Like Substance in the Human Pituitary Gland*

A monoclonal mouse antibody to human chorionic gonadotropin (hCG) was used in a modified unlabeled antibody enzyme-bridge staining method to demonstrate the localization of hCG in normal human placenta, pituitary gland, and six pituitary chromophobe adenomas. Mouse ascitic fluid containing monoclonal antibody could be diluted up to 1:500,000 for detection of hCG in the syncytiotrophoblast, whereas no staining was observed in the pituitary or adenomas even with high antibody concentrations (dilutions from 1:500 upward). Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies are suitable for immunohistochemical localization of antigens in tissues.

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Effects of Ovaprim, Pituitary Gland Extract and Human Chorionic Gonadotropin on Testosterone, 11-Ketotestosterone,and 17β-Estradiol hormones of catfish (Clarias gariepinus)

Egyptian Journal for Aquaculture ◽

10.21608/eja.2020.52848.1043 ◽

2020 ◽

Vol 0 (0) ◽

pp. 1-16

Author(s):

Eman Zaki ◽

Haytham Abd El-Ghaffar

Keyword(s):

Pituitary Gland ◽

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Clarias Gariepinus ◽

Gland Extract ◽

17Β Estradiol

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IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN CHORIONIC GONADOTROPIN

Journal of Experimental Medicine ◽

10.1084/jem.115.2.289 ◽

1962 ◽

Vol 115 (2) ◽

pp. 289-294 ◽

Cited By ~ 149

Author(s):

A. R. Midgley ◽

G. B. Pierce

Keyword(s):

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Hydatidiform Mole ◽

Immunohistochemical Localization ◽

Formalin Fixation ◽

Cell Of Origin ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Immunohistochemical Techniques ◽

Formalin Fixed

Through the use of immunohistochemical techniques, human chorionic gonadotropin has been localized to syncytiotrophoblastic cells of immature placenta, hydatidiform mole, chorioadenoma destruens, and choriocarcinoma. No gonadotropin has been detected in cytotrophoblast. Evidence is discussed which suggests that syncytiotrophoblast is the cell of origin of human chorionic gonadotropin. The observation that formalin fixation did not alter the ability of human chorionic gonadotropin to react with its specific antibody permitted the study of formalin-fixed paraffin-embedded tissues stored in the tissue collection. In addition, the excellence of histologic preparations following formalin fixation facilitated cytologic identification.

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The corticotrophic cells of the canine pituitary gland in pituitary-dependent hyperadrenocorticism

Journal of Endocrinology ◽

10.1677/joe.0.0960303 ◽

1983 ◽

Vol 96 (2) ◽

pp. 303-309 ◽

Cited By ~ 7

Author(s):

A. M. McNicol ◽

H. Thomson ◽

C. J. R. Stewart

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Pituitary Gland ◽

Cushing’S Disease ◽

Cushing's Disease ◽

Human Pituitary ◽

Human Pituitary Gland ◽

Single Entity ◽

Pituitary Glands ◽

Corticotrophic Cells

The distribution of specifically stained corticotrophic cells has been studied in the pituitary glands of 11 dogs with pituitary-dependent hyperadrenocorticism. The results suggest that the disease is not a single entity, and that some cases are caused by primary abnormality of the pituitary gland whereas others appear to be the result of dysfunction of the hypothalamus or central nervous system. The patterns correspond closely to those demonstrated in the human pituitary gland in Cushing's disease, and confirm that the canine disease is a useful model for the study of the pathogenesis of the variants of the condition.

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