Regulation of insulin-like growth factor (IGF)-binding protein-4 availability in normal human osteoblast-like cells: role of endogenous IGFs.

1995 ◽  
Vol 80 (1) ◽  
pp. 104-110
Author(s):  
S K Durham ◽  
D D De León ◽  
R Okazaki ◽  
B L Riggs ◽  
C A Conover
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Human Osteoblast ◽  
Igf Binding ◽  
Normal Human ◽  
Normal Human Osteoblast ◽  
Igf Binding Protein

scholarly journals Insulin-Like Growth Factor (IGF) Binding Protein-2, Independently of IGF-1, Induces GLUT-4 Translocation and Glucose Uptake in 3T3-L1 Adipocytes

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Biruhalem Assefa ◽  
Ayman M. Mahmoud ◽  
Andreas F. H. Pfeiffer ◽  
Andreas L. Birkenfeld ◽  
Joachim Spranger ◽  
...  
Keyword(s):  
Growth Factor ◽  
Glucose Uptake ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Glut 4 ◽  
Igf Binding ◽  
Glut 4 Translocation ◽  
Igf Binding Protein ◽  
The Impact

Insulin-like growth factor binding protein-2 (IGFBP-2) is the predominant IGF binding protein produced during adipogenesis and is known to increase the insulin-stimulated glucose uptake (GU) in myotubes. We investigated the IGFBP-2-induced changes in basal and insulin-stimulated GU in adipocytes and the underlying mechanisms. We further determined the role of insulin and IGF-1 receptors in mediating the IGFBP-2 and the impact of IGFBP-2 on the IGF-1-induced GU. Fully differentiated 3T3-L1 adipocytes were treated with IGFBP-2 in the presence and absence of insulin and IGF-1. Insulin, IGF-1, and IGFBP-2 induced a dose-dependent increase in GU. IGFBP-2 increased the insulin-induced GU after long-term incubation. The IGFBP-2-induced impact on GU was neither affected by insulin or IGF-1 receptor blockage nor by insulin receptor knockdown. IGFBP-2 significantly increased the phosphorylation of PI3K, Akt, AMPK, TBC1D1, and PKCζ/λand induced GLUT-4 translocation. Moreover, inhibition of PI3K and AMPK significantly reduced IGFBP-2-stimulated GU. In conclusion, IGFBP-2 stimulates GU in 3T3-L1 adipocytes through activation of PI3K/Akt, AMPK/TBC1D1, and PI3K/PKCζ/λ/GLUT-4 signaling. The stimulatory effect of IGFBP-2 on GU is independent of its binding to IGF-1 and is possibly not mediated through the insulin or IGF-1 receptor. This study highlights the potential role of IGFBP-2 in glucose metabolism.

Role of insulin-like growth factor-I (IGF-I) receptor, IGF-I, and IGF binding protein-2 in human colorectal cancers

1998 ◽  
Vol 8 (6) ◽  
pp. 473-479 ◽  
Author(s):  
L. Mishra ◽  
B. Bass ◽  
B.S. Ooi ◽  
A. Sidawy ◽  
L. Korman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Colorectal Cancers ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Igf Binding ◽  
Growth Factor I ◽  
Igf Binding Protein
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