scholarly journals A One-Tube Polymerase Chain Reaction Protocol Demonstrates CC Chemokine Overexpression in Graves’ Disease Glands

1999 ◽  
Vol 84 (8) ◽  
pp. 2873-2882
Author(s):  
Yaqoub Ashhab ◽  
Orlando Dominguez ◽  
Mireia Sospedra ◽  
Carme Roura-Mir ◽  
Anna Lucas-Martín ◽  
...  
Keyword(s):  
Human Leukocyte ◽  
Needle Aspiration ◽  
Graves Disease ◽  
Tissue Samples ◽  
Autoimmune Thyroid ◽  
Leukocyte Antigen ◽  
Cc Chemokine ◽  
Inflammatory Protein ◽  
Cc Chemokines ◽  
Polymerase Chain

An adaptation of mixed oligonucleotide primed amplification of complementary DNA to detect the profile of CC chemokines in biological samples is presented. By introducing normalization, two correction coefficients, performing a single amplification reaction, and five parallel hybridizations, intrasample and intersample comparisons can be reliably made. This protocol of single tube PCR CC chemokine profiling was applied to tissue samples from an autoimmune thyroid condition, Graves’ disease, and from a nonautoimmune condition, multinodular goiter. Results demonstrate overexpression of CC chemokines in Graves’ disease, statistically significant for macrophage inflammatory protein-1α and -1β, which correlated with the aberrant human leukocyte antigen class II expression by thyrocytes, as assessed by flow cytometry. Overexpression of CC chemokines probably plays a major role in determining the characteristics of the lymphocytes migrating to the thyroid gland and influences the course of the disease. The study of chemokine profile should be more informative than the study of isolated chemokines and cytokines, and as it can be applied to fine needle aspiration biopsies, it may be useful to clinical research.

scholarly journals Frequency of alleles associated with celiac disease in patients with autoimmune thyroid disease

2021 ◽  
Vol 34 ◽  
Author(s):  
Clédia Silveira Flores da SILVA ◽  
Natalia Rodrigues CARDOZO ◽  
Raíssa ZANATTA ◽  
Augusto SCHNEIDER ◽  
Carlos Castilho de BARROS ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Gastrointestinal Symptoms ◽  
Human Leukocyte ◽  
Thyroid Diseases ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Leukocyte Antigen ◽  
Polymerase Chain ◽  
Genetic Profiles

ABSTRACT Objective To determine the frequency of Human leukocyte antigen alleles and to verify the association of the presence of these alleles with symptoms and other diseases related to celiac disease in patients with autoimmune thyroid diseases. Methods A questionnaire on the symptoms and diseases associated with celiac disease was applied. Genomic deoxyribonucleic acid was extracted by collecting cells from the oral mucosa. The alleles (DQA1*0501; DQB1*0201; DRB1*04) were identified by means of the polymerase chain reaction. Results A total of 110 patients with autoimmune thyroid diseases participated in this study. It was observed that 66.4% of the individuals carried at least one of the alleles assessed and that 58.2% of the individuals were positive for at least one of the DQ2 alleles (DQA1*0501; DQB1*0201) and out of these 18.2% were positive for both DQ2 alleles (DQA1*0501; DQB1*0201). With regard to DQ8 (DRB1*04), 21.8% of the studied population was positive for this allele and 3.6% was positive for both DQ2 (DQA1*0501; DQB1*0201) and DQ8 (DRB1*04). A significant association was found between the presence of the DRB1*04 allele and gastrointestinal symptoms (p=0.02). A significant association of the DRB1*04 allele with type 1 diabetes mellitus (p=0.02) was observed. Conclusion The genetic profiles most commonly associated with celiac disease, such as DQ2 (DQA1*0501; DQB1*0201) and DQ8 (DRB1*04) were around 20.0% prevalent in the studied population. These are risk haplotypes for celiac disease especially when symptoms and diseases related to celiac disease are present. Therefore, it is important to screen patients to investigate a potential diagnosis for celiac disease.

scholarly journals Regression Mapping of Association between the Human Leukocyte Antigen Region and Graves Disease

2005 ◽  
Vol 76 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Matthew J. Simmonds ◽  
Joanna M.M. Howson ◽  
Joanne M. Heward ◽  
Heather J. Cordell ◽  
Helen Foxall ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Graves Disease ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen Region ◽  
Regression Mapping

scholarly journals Binding of Human Thyrotropin Receptor Peptides to a Graves’ Disease-Predisposing Human Leukocyte Antigen Class II Molecule

2000 ◽  
Vol 85 (3) ◽  
pp. 1176-1179 ◽  
Author(s):  
Yoshikuni Sawai ◽  
Leslie J. DeGroot
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Class Ii ◽  
Hla Class Ii ◽  
Graves Disease ◽  
T Cell Repertoire ◽  
Thyrotropin Receptor ◽  
Cell Repertoire ◽  
Peptide Epitopes ◽  
Leukocyte Antigen

Abstract Abstract There are many reports that Graves’ disease (GD) is associated with certain human leukocyte antigen (HLA) molecules, in particular DR3. Here we examined the characteristics of binding of human TSH receptor (TSHR) peptides to this disease-associated HLA class II molecule. DR3 molecules bind TSHR immuonodominant peptide epitopes with intermediate affinity. On the contrary, DR3 binds nonimmunogenic peptides either with poor affinity or not at all, with one exceptional peptide that has extremely high affinity. These results suggest that susceptibility to GD associated with inheritance of a specific HLA class II gene is due to the influence of the HLA molecule-TSHR peptide complex on the T cell repertoire.

scholarly journals The Human Leukocyte Antigen HLA DRB3∗0202/DQA1∗0501 Haplotype Is Associated with Graves’ Disease in African Americans

2000 ◽  
Vol 85 (4) ◽  
pp. 1545-1549 ◽  
Author(s):  
Qiao-Yi Chen ◽  
David Nadell ◽  
Xian-Yang Zhang ◽  
Anjli Kukreja ◽  
Yao-Jin Huang ◽  
...  
Keyword(s):  
African Americans ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Graves Disease ◽  
Leukocyte Antigen

scholarly journals The unusual association of Graves' disease, chronic spontaneous urticaria, and premature ovarian failure: report of a case and HLA haplotype characterization

2013 ◽  
Vol 57 (9) ◽  
pp. 748-752 ◽  
Author(s):  
Rosaria Maddalena Ruggeri ◽  
Giuseppe Vita ◽  
Anna Grazia D'Angelo ◽  
Paolina Quattrocchi ◽  
Rosaria Certo ◽  
...  
Keyword(s):  
Premature Ovarian Failure ◽  
Genetic Background ◽  
Marked Improvement ◽  
Human Leukocyte ◽  
Ovarian Failure ◽  
Chronic Spontaneous Urticaria ◽  
Graves Disease ◽  
Leukocyte Antigen ◽  
Unusual Association ◽  
Symptoms And Signs

Chronic spontaneous urticaria (CSU), defined as the occurrence of spontaneous wheals for more than six weeks, has been associated with autoimmune diseases. Herein, we report the unusual association of CSU, Graves' disease, and premature ovarian failure. Human leukocyte antigen (HLA) studies were performed. A 36-year-old woman presented symptoms and signs of hyperthyroidism for three months. In the same period, the patient complained of widespread urticarial wheals, intensely itchy, and poorly responsive to therapy with antihistaminic agents. Hyperthyroidism was confirmed biochemically, and treatment with methimazole was started. As hyperthyroidism improved, a marked improvement in her urticaria was also observed. However, the patient continued to complain of amenorrhea. Endocrine evaluation, at the age 38, was consistent with premature ovarian failure. This is the first report of coexistence of GD, CSU, and POF. The genetic background of such unusual association is a specific combination of HLA.

scholarly journals An Intronic HCP5 Variant Is Associated With Age of Onset and Susceptibility to Graves Disease in UK and Polish Cohorts

2020 ◽  
Vol 105 (9) ◽  
pp. e3277-e3284 ◽  
Author(s):  
Laura Claire Lane ◽  
Aleksander Kuś ◽  
Tomasz Bednarczuk ◽  
Artur Bossowski ◽  
Jacek Daroszewski ◽  
...  
Keyword(s):  
Age Of Onset ◽  
Meta Analysis ◽  
Human Leukocyte ◽  
Graves Disease ◽  
Ribonucleic Acids ◽  
Young Onset ◽  
Leukocyte Antigen ◽  
Genotype Frequencies ◽  
The Uk

Abstract Context The genetic background of young-onset Graves disease (GD) remains largely unknown. An intronic variant in human leukocyte antigen (HLA) complex P5 (HCP5) has previously been associated with GD susceptibility and age of onset in a cohort of Polish patients. Objective We aimed to investigate the association of the HCP5 variant rs3094228 with GD susceptibility and age of onset in a UK cohort and conduct a meta-analysis of UK and Polish data. Design and Participants rs3094228 was genotyped in 469 UK patients with GD using Taqman chemistry. Genotype frequencies were compared with genotypic data available from the Wellcome Trust case-control consortium using logistic regression analysis. To determine whether rs3094228 is independently associated with age of GD onset, the HLA DRB1*0301 tagging variant, rs535777, was also genotyped. Results The C allele of rs3094228 was overrepresented in the UK GD cohort compared with controls (P allele=5.08 × 10–9, odds ratio 1.76; [95% confidence interval, 1.46-2.13]). This association was more marked in young-onset GD (<30 years) (P allele=1.70 × 10–10 vs P allele=0.0008). The meta-analysis of UK and Polish data supported the association of the C allele with GD susceptibility (P allele=1.79 × 10–5) and age of onset (P allele=5.63 × 10–8). Haplotype analysis demonstrated that rs3094228 is associated with age of GD onset (P = 2.39 × 10-6) independent of linkage disequilibrium with HLA DRB1*0301. Conclusion The rs3094228 HCP5 polymorphism is independently associated with GD susceptibility and age of onset in a UK GD cohort. Our findings indicate a potential role of long noncoding ribonucleic acids, including HCP5, in GD pathogenesis, particularly in the younger population.

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