scholarly journals Mouse intraflagellar transport proteins regulate both the activator and repressor functions of Gli transcription factors

Development ◽  
2005 ◽  
Vol 132 (13) ◽  
pp. 3103-3111 ◽  
Author(s):  
A. Liu
Keyword(s):  
Transcription Factors ◽  
Intraflagellar Transport ◽  
Transport Proteins ◽  
Gli Transcription Factors

Hedgehog-induced ciliary trafficking of kinesin-4 motor KIF7 requires intraflagellar transport but not KIF7’s microtubule binding

2021 ◽  
Author(s):  
Yang Yue ◽  
Martin F. Engelke ◽  
T. Lynne Blasius ◽  
Kristen J. Verhey
Keyword(s):  
Transcription Factors ◽  
Signaling Pathway ◽  
Primary Cilium ◽  
Hedgehog Signaling ◽  
Intraflagellar Transport ◽  
Hedgehog Signaling Pathway ◽  
Microtubule Binding ◽  
Pathway Activation ◽  
Gli Transcription Factors ◽  
Ciliary Localization

The kinesin-4 motor KIF7 is a conserved regulator of the Hedgehog signaling pathway. In vertebrates, Hedgehog signaling requires the primary cilium, and KIF7 and Gli transcription factors accumulate at the cilium tip in response to Hedgehog activation. Unlike conventional kinesins, KIF7 is an immotile kinesin and its mechanism of ciliary accumulation is unknown. We generated KIF7 variants with altered microtubule binding or motility. We demonstrate that microtubule binding of KIF7 is not required for the increase in KIF7 or Gli localization at the cilium tip in response to Hedgehog signaling. In addition, we show that the immotile behavior of KIF7 is required to prevent ciliary localization of Gli transcription factors in the absence of Hedgehog signaling. Using an engineered kinesin-2 motor that enables acute inhibition of intraflagellar transport (IFT), we demonstrate that kinesin-2 KIF3A/KIF3B/KAP mediates the translocation of KIF7 to the cilium tip in response to Hedgehog pathway activation. Together, these results suggest that KIF7’s role at the tip of the cilium is unrelated to its ability to bind to microtubules.

scholarly journals Intraflagellar transport is deeply integrated in hedgehog signaling

2018 ◽  
Vol 29 (10) ◽  
pp. 1178-1189 ◽  
Author(s):  
Thibaut Eguether ◽  
Fabrice P. Cordelieres ◽  
Gregory J. Pazour
Keyword(s):  
Transcription Factors ◽  
Membrane Protein ◽  
Hedgehog Signaling ◽  
Primary Cilia ◽  
Intraflagellar Transport ◽  
Hedgehog Pathway ◽  
Gli Transcription Factors ◽  
Cilia Formation ◽  
Mutant Cells

The vertebrate hedgehog pathway is organized in primary cilia, and hedgehog components relocate into or out of cilia during signaling. Defects in intraflagellar transport (IFT) typically disrupt ciliary assembly and attenuate hedgehog signaling. Determining whether IFT drives the movement of hedgehog components is difficult due to the requirement of IFT for building cilia. Unlike most IFT proteins, IFT27 is dispensable for cilia formation but affects hedgehog signaling similarly to other IFTs, allowing us to examine its role in the dynamics of signaling. Activating signaling at points along the pathway in Ift27 mutant cells showed that IFT is extensively involved in the pathway. Similar analysis of Bbs mutant cells showed that BBS proteins participate at many levels of signaling but are not needed to concentrate Gli transcription factors at the ciliary tip. Our analysis showed that smoothened delivery to cilia does not require IFT27, but the role of other IFTs is not known. Using a rapamycin-induced dimerization system to sequester IFT-B proteins at the mitochondria in cells with fully formed cilia did not affect the delivery of Smo to cilia, suggesting that this membrane protein may not require IFT-B for delivery.

Pirfenidone exerts anti-fibrotic effects through Inhibition of GLI transcription factors

Pneumologie ◽  
2018 ◽  
Vol 72 (S 01) ◽  
pp. S114-S115
Author(s):  
M Wygrecka ◽  
M Didiasova ◽  
R Singh ◽  
J Wilhelm ◽  
G Kwapiszewska ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gli Transcription Factors

scholarly journals Fuzzy modeling reveals a dynamic self-sustaining network of the GLI transcription factors controlling important metabolic regulators in adult mouse hepatocytes

2015 ◽  
Vol 11 (8) ◽  
pp. 2190-2197 ◽  
Author(s):  
Wolfgang Schmidt-Heck ◽  
Madlen Matz-Soja ◽  
Susanne Aleithe ◽  
Eugenia Marbach ◽  
Reinhard Guthke ◽  
...  
Keyword(s):  
Fuzzy Logic ◽  
Transcription Factors ◽  
Regulatory Network ◽  
Adult Mouse ◽  
Dynamic Features ◽  
Hedgehog Signalling ◽  
Gli Transcription Factors ◽  
Mouse Hepatocytes ◽  
Metabolic Regulators ◽  
Modelling Approach

The Hedgehog signalling-driven Gli transcription factors in hepatocytes form a regulatory network identified by a fuzzy-logic modelling approach. The network explains dynamic features important for hepatocyte function and fate.

Epigenetic control of KRAS-induced transformation by GLI transcription factors

Pancreatology ◽  
2013 ◽  
Vol 13 (2) ◽  
pp. e25
Author(s):  
M.G. Fernandez-Barrena ◽  
L.L. Almada ◽  
M.E. Fernandez-Zapico
Keyword(s):  
Transcription Factors ◽  
Epigenetic Control ◽  
Gli Transcription Factors

Hedgehog signalling in the mouse requires intraflagellar transport proteins

Nature ◽  
2003 ◽  
Vol 426 (6962) ◽  
pp. 83-87 ◽  
Author(s):  
Danwei Huangfu ◽  
Aimin Liu ◽  
Andrew S. Rakeman ◽  
Noel S. Murcia ◽  
Lee Niswander ◽  
...  
Keyword(s):  
Intraflagellar Transport ◽  
Transport Proteins ◽  
Hedgehog Signalling

scholarly journals Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis

2016 ◽  
Vol 291 (49) ◽  
pp. 25749-25760 ◽  
Author(s):  
Meng Wu ◽  
Lishann Ingram ◽  
Ezequiel J. Tolosa ◽  
Renzo E. Vera ◽  
Qianjin Li ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Androgen Receptor ◽  
Basal Cells ◽  
Oncogenic Transformation ◽  
Gli Transcription Factors

scholarly journals Dissecting the functions of Gli transcription factors in T-cell development

Cell Cycle ◽  
2010 ◽  
Vol 9 (22) ◽  
pp. 4429-4428
Author(s):  
Alberto Varas ◽  
Rosa Sacedón
Keyword(s):  
Transcription Factors ◽  
T Cell ◽  
T Cell Development ◽  
Cell Development ◽  
Gli Transcription Factors
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