Expression of homeobox gene Hox 1.1 during mouse embryogenesis

Development ◽  
1988 ◽  
Vol 104 (Supplement) ◽  
pp. 187-195 ◽  
Author(s):  
Kathleen A. Mahon ◽  
Heiner Westphal ◽  
Peter Gruss
Keyword(s):  
In Situ Hybridization ◽  
Homeobox Genes ◽  
Homeobox Gene ◽  
Spinal Ganglia ◽  
Spatial And Temporal Patterns ◽  
Rna Transcripts ◽  
Positional Identity ◽  
Specific Manner ◽  
Dorsal Spinal

Many of the genes controlling segmentation and pattern formation in Drosophila contain a conserved 183 bp sequence known as the homeobox. Homeobox sequences have been found in a range of metazoan species, including the vertebrates mouse and man. This striking conservation suggests that homeobox genes may play a fundamental role in developmental processes. If this is the case then it might be expected that vertebrate homeobox genes will be differentially expressed during embryogenesis and that the timing of their expression will coincide with major morphogenetic events. Here the spatial and temporal patterns of expression of murine homeobox genes will be explored, concentrating on the Hox 1.1 gene as an example. Using in situ hybridization to localize RNA transcripts, it has been found that Hox 1.1 is expressed in a region-specific manner during the formation and differentiation of the embryonic anteroposterior axis. Although striking patterns of expression of Hox 1.1 and other homeobox genes are seen in overtly segmented structures of the embryo (i.e. somites, prevertebral elements, neural tube and dorsal spinal ganglia) expression is also seen in tissues with no obvious segmental origin. The results suggest that homeobox genes probably do not play an exclusive role in segmentation in vertebrates, but are consistent with a role in the assignment of positional identity along the axis of the embryo.

scholarly journals Isolation, Characterization, and in Situ Hybridization Studies of the Abundantly Transcribed Potato (Solanum tuberosum `Superior') Homeobox cDNA POTH1

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 453A-453
Author(s):  
Jennifer K. Hart ◽  
David J. Hannapel
Keyword(s):  
In Situ Hybridization ◽  
Potato Plant ◽  
Messenger Rna ◽  
Developmental Process ◽  
Homeobox Genes ◽  
Homeobox Gene ◽  
Vascular Cambium ◽  
Northern Analysis ◽  
Root Tips

Homeobox genes contain sequences coding for DNA-binding motifs. These sequences are highly conserved across both the animal and plant kingdoms. Members of this gene family code for transcription factors that are key regulators of developmental organization. In an attempt to further elucidate the developmental process of tuberization in the potato plant, a full-length homeobox cDNA has been isolated via sequence homology from an early tuberization stage cDNA library constructed from 4-day axillary bud tubers. This cDNA, POTH1, has been sequenced and characterized by Southern blotting, northern analysis, sequence comparison, and in situ hybridization. POTH1 is shown to be a class I homeobox gene with 45% overall similarity to Kn-1 of maize and 73% match in the homeobox region. Messenger RNA accumulation studies indicate that POTH1 mRNA, unlike most homeobox transcripts, is not limited to a particular organ or developmental stage. Instead, POTH1 mRNA accumulates in rapidly growing cells of the potato plant: the apical meristems, the vascular cambium, the edges of young leaves, axillary buds, and root tips. In situ studies indicate accumulation of POTH1 mRNA in the tunica and corpus layers of the apical dome of the shoot apex and the stolon apex. In the stolon, growth and proliferation of the parenchymal cells associated with the vascular cambium contribute to swelling during early stages of tuberization, and this tissue accumulates POTH1 mRNA. It is possible that POTH1 may be posttranscriptionally regulated in a particular organ or stage of growth, or that it is involved in a wider range of growth processes than most plant homeobox genes.

The testis-specific transcript (ferT) of the tyrosine kinase FER is expressed during spermatogenesis in a stage-specific manner

1990 ◽  
Vol 10 (9) ◽  
pp. 5021-5025
Author(s):  
E Keshet ◽  
A Itin ◽  
K Fischman ◽  
U Nir
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Kinase ◽  
Tyrosine Kinases ◽  
Meiotic Prophase ◽  
Hybridization Analysis ◽  
Pachytene Stage ◽  
Specific Transcript ◽  
Specific Manner

ferT is a testis-specific transcript of FER encoding a truncated version of the potential tyrosine kinase. Using in situ hybridization analysis, we found that ferT was transiently expressed during spermatogenesis and that expression was restricted to spermatocytes at the pachytene stage of meiotic prophase. This pattern of expression is unprecedented by other tyrosine kinases and suggests a role for ferT in a particular stage of spermatogenesis.

scholarly journals In Situ Hybridization Analysis of ZPK Gene Expression During Murine Embryogenesis

1997 ◽  
Vol 45 (1) ◽  
pp. 107-118 ◽  
Author(s):  
André Nadeau ◽  
Gilles Grondin ◽  
Richard Blouin
Keyword(s):  
In Situ Hybridization ◽  
Northern Blot Analysis ◽  
Spatial And Temporal Patterns ◽  
Serine Threonine Kinase ◽  
Teratocarcinoma Cell ◽  
Threonine Kinase ◽  
Cell Lineages ◽  
Polymerase Chain

ZPK is a recently described protein serine/threonine kinase that has been originally identified from a human teratocarcinoma cell line by the polymerase chain reaction and whose function in signal transduction has not yet been elucidated. To investigate the potential role of this protein kinase in developmental processes, we have analyzed the spatial and temporal patterns of expression of the ZPK gene in mouse embryos of different gestational ages. Northern blot analysis revealed a single mRNA species of about 3.5 KB from Day 11 of gestation onwards. In situ hybridization studies demonstrated strong expression of ZPK mRNA in brain and in a variety of embryonic organs that rely on epithelio-mesenchymal interactions for their development, including skin, intestine, pancreas, and kidney. In these tissues, the ZPK mRNA was localized primarily in areas composed of specific types of differentiating cells, and this expression appeared to be upregulated at a time concomitant with the onset of terminal differentiation. Taken together, these observations raise the possibility that the ZPK gene product is involved in the establishment and/or maintenance of a fully cytodifferentiated state in a variety of cell lineages.

Ghox 4.7: a chick homeobox gene expressed primarily in limb buds with limb-type differences in expression

Development ◽  
1991 ◽  
Vol 112 (3) ◽  
pp. 791-806 ◽  
Author(s):  
S. Mackem ◽  
K.A. Mahon
Keyword(s):  
Expression Patterns ◽  
Homeobox Genes ◽  
Homeobox Gene ◽  
Limb Bud ◽  
Limb Buds ◽  
Limb Morphogenesis ◽  
Degenerate Oligonucleotide ◽  
Polymerase Chain ◽  
Anterior Posterior

Homeobox genes play a key role in specifying the segmented body plan of Drosophila, and recent work suggests that at least several homeobox genes may play a regulatory role during vertebrate limb morphogenesis. We have used degenerate oligonucleotide primers from highly conserved domains in the homeobox motif to amplify homeobox gene segments from chick embryo limb bud cDNAs using the polymerase chain reaction. Expression of a large number of homeobox genes (at least 17) is detected using this approach. One of these genes contains a novel homeobox loosely related to the Drosophila Abdominal B class, and was further analyzed by determining its complete coding sequence and evaluating its expression during embryogenesis by in situ hybridization. Based on sequence and expression patterns, we have designated this gene as Ghox 4.7 and believe that it is the chick homologue of the murine Hox 4.7 gene (formerly Hox 5.6). Ghox 4.7 is expressed primarily in limb buds during development and shows a striking spatial restriction to the posterior zone of the limb bud, suggesting a role in specifying anterior-posterior pattern formation. In chick, this gene also displays differences in expression between wing and leg buds, raising the possibility that it may participate in specifying limb-type identity.

scholarly journals The testis-specific transcript (ferT) of the tyrosine kinase FER is expressed during spermatogenesis in a stage-specific manner.

1990 ◽  
Vol 10 (9) ◽  
pp. 5021-5025 ◽  
Author(s):  
E Keshet ◽  
A Itin ◽  
K Fischman ◽  
U Nir
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Kinase ◽  
Tyrosine Kinases ◽  
Meiotic Prophase ◽  
Hybridization Analysis ◽  
Pachytene Stage ◽  
Specific Transcript ◽  
Specific Manner

ferT is a testis-specific transcript of FER encoding a truncated version of the potential tyrosine kinase. Using in situ hybridization analysis, we found that ferT was transiently expressed during spermatogenesis and that expression was restricted to spermatocytes at the pachytene stage of meiotic prophase. This pattern of expression is unprecedented by other tyrosine kinases and suggests a role for ferT in a particular stage of spermatogenesis.

In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas

2011 ◽  
Vol 58 (2) ◽  
pp. 225-233 ◽  
Author(s):  
Tatiana N Libório ◽  
Thais Acquafreda ◽  
Luciana F Matizonkas-Antonio ◽  
Maria G Silva-Valenzuela ◽  
Alberto R Ferraz ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Squamous Cell ◽  
Expression Patterns ◽  
Homeobox Genes ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas ◽  
Hybridization Detection

Analysis of the Dorsal Spinal Cord Synaptic Architecture by Combined Proteome Analysis and in Situ Hybridization

2005 ◽  
Vol 4 (2) ◽  
pp. 238-249 ◽  
Author(s):  
Mathias Dreger ◽  
Joanna Mika ◽  
Annette Bieller ◽  
Ricarda Jahnel ◽  
Clemens Gillen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
In Situ Hybridization ◽  
Proteome Analysis ◽  
Dorsal Spinal Cord ◽  
Dorsal Spinal

scholarly journals Region-specific expression in early chick and mouse embryos of Ghox-lab and Hox 1.6, vertebrate homeobox-containing genes related to Drosophila labial

Development ◽  
1990 ◽  
Vol 108 (1) ◽  
pp. 47-58
Author(s):  
O.H. Sundin ◽  
H.G. Busse ◽  
M.B. Rogers ◽  
L.J. Gudas ◽  
G. Eichele
Keyword(s):  
In Situ Hybridization ◽  
Homeobox Gene ◽  
Mouse Embryos ◽  
Mouse Development ◽  
Temporal And Spatial Distribution ◽  
Specific Expression ◽  
Early Embryos ◽  
Close Relationship ◽  
Additional Sequence

A chick gene homologous to the Drosophila homeobox gene labial has been cloned and sequenced. Regions of additional sequence identity outside of the homeobox reveal a close relationship to the mouse gene Hox 1.6. Northern blot analysis demonstrates that Ghox-lab and Hox 1.6 transcripts are both present at high levels during early stages of chick and mouse development, with a subsequent decline in abundance to very low levels by the time limb mesenchyme begins to differentiate. In situ hybridization analysis of chick embryos shows intense expression of Ghox-lab mRNA by Hamburger and Hamilton stage 4 (avian ‘mid gastrula’) and by stage 6 (pre-somitic neural plate) with expression decreasing shortly thereafter. The pattern of Ghox-lab RNA expression in these early embryos divides the embryo into an anterior and a posterior compartment. At stage 6, considerable signal is observed in the posterior two thirds of the embryo, while none is detected in the anterior third which is fated to become the head. This pattern is purely regional in nature, and does not follow boundaries defined by known tissue types. In situ hybridization of Hox 1.6 probes to mouse embryos of day 7.5 or 8.0 indicate that the Hox 1.6 transcript has a temporal and spatial distribution very similar to that of Ghox-lab in the chick embryo.

scholarly journals A technical review and guide to RNA fluorescence in situ hybridization

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8806
Author(s):  
Alexander P. Young ◽  
Daniel J. Jackson ◽  
Russell C. Wyeth
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Messenger Rna ◽  
Cultured Cells ◽  
Tissue Preparation ◽  
Functional Roles ◽  
Rna Transcripts ◽  
Methods Development ◽  
Background Removal

RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations. As the technique has been developed over time, an ever-increasing number of divergent protocols have been published. There is now a broad selection of options available to facilitate proper tissue preparation, hybridization, and post-hybridization background removal to achieve optimal results. Here we review the technical aspects of RNA-FISH, examining the most common methods associated with different sample types including cytological preparations and whole-mounts. We discuss the application of commonly used reagents for tissue preparation, hybridization, and post-hybridization washing and provide explanations of the functional roles for each reagent. We also discuss the available probe types and necessary controls to accurately visualize gene expression. Finally, we review the most recent advances in FISH technology that facilitate both highly multiplexed experiments and signal amplification for individual targets. Taken together, this information will guide the methods development process for investigators that seek to perform FISH in organisms that lack documented or optimized protocols.

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