zfh-1 is required for germ cell migration and gonadal mesoderm development in Drosophila

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 655-666 ◽  
Author(s):  
H.T. Broihier ◽  
L.A. Moore ◽  
M. Van Doren ◽  
S. Newman ◽  
R. Lehmann
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Ectopic Expression ◽  
Homeodomain Protein ◽  
Mesoderm Development ◽  
Visceral Mesoderm ◽  
Temporal Course ◽  
Germ Cell Migration

In Drosophila as well as many vertebrate systems, germ cells form extraembryonically and migrate into the embryo before navigating toward gonadal mesodermal cells. How the gonadal mesoderm attracts migratory germ cells is not understood in any system. We have taken a genetic approach to identify genes required for germ cell migration in Drosophila. Here we describe the role of zfh-1 in germ cell migration to the gonadal mesoderm. In zfh-1 mutant embryos, the initial association of germ cells and gonadal mesoderm is blocked. Loss of zfh-1 activity disrupts the development of two distinct mesodermal populations: the caudal visceral mesoderm and the gonadal mesoderm. We demonstrate that the caudal visceral mesoderm facilitates the migration of germ cells from the endoderm to the mesoderm. Zfh-1 is also expressed in the gonadal mesoderm throughout the development of this tissue. Ectopic expression of Zfh-1 is sufficient to induce additional gonadal mesodermal cells and to alter the temporal course of gene expression within these cells. Finally, through analysis of a tinman zfh-1 double mutant, we show that zfh-1 acts in conjunction with tinman, another homeodomain protein, in the specification of lateral mesodermal derivatives, including the gonadal mesoderm.

scholarly journals Evidence for a role of matrix metalloproteinases and their inhibitors in primordial germ cell migration

Andrology ◽  
2013 ◽  
Vol 1 (5) ◽  
pp. 779-786 ◽  
Author(s):  
A. Díez-Torre ◽  
M. Díaz-Núñez ◽  
C. Eguizábal ◽  
U. Silván ◽  
J. Aréchaga
Keyword(s):  
Cell Migration ◽  
Matrix Metalloproteinases ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Migration

scholarly journals The role of cadherins during primordial germ cell migration and early gonad formation in the mouse

2000 ◽  
Vol 91 (1-2) ◽  
pp. 143-152 ◽  
Author(s):  
Michael R Bendel-Stenzel ◽  
Miranda Gomperts ◽  
Robert Anderson ◽  
Janet Heasman ◽  
Christopher Wylie
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Migration

Identification of genes controlling germ cell migration and embryonic gonad formation in Drosophila

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 667-678 ◽  
Author(s):  
L.A. Moore ◽  
H.T. Broihier ◽  
M. Van Doren ◽  
L.B. Lunsford ◽  
R. Lehmann
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Cell Types ◽  
Drosophila Embryo ◽  
Specific Cell ◽  
Embryonic Patterning ◽  
Phenotypic Analysis ◽  
Embryonic Gonad ◽  
Germ Cell Migration

Gonadogenesis in the Drosophila embryo is a complex process involving numerous cellular migratory steps and cell-cell interactions. The mechanisms guiding germ cells to move through, recognize and adhere to specific cell types are poorly understood. In order to identify genes that are required for these processes, we have conducted an extensive mutagenesis of the third chromosome and screened for mutations disrupting germ cell migration at any point in embryonic development. Phenotypic analysis of these mutants demonstrates that germ cell migration can be broken down into discrete developmental steps, with each step requiring a specific set of genes. Many of these genes are involved in the development of gonadal mesoderm, the tissue that associates with germ cells to form the embryonic gonad. Moreover, mutations that we isolated affecting embryonic patterning as well as germ cell migration suggest that the origin of gonadal mesoderm lies within the eve domain of the developing mesoderm.

scholarly journals Primordial Germ Cell Migration in the Rat: Preliminary Evidence for a Role of Galactosyltransferase1

2004 ◽  
Vol 71 (6) ◽  
pp. 1822-1827 ◽  
Author(s):  
Soma Bandyopadhyay ◽  
Sutapa Banerjee ◽  
Alok K. Pal ◽  
Sourendra K. Goswami ◽  
Baidyanath Chakravarty ◽  
...  
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Preliminary Evidence ◽  
Germ Cell Migration

Identification of X-Linked Genes Required for Migration and Programmed Cell Death of Drosophila melanogaster Germ Cells

Genetics ◽  
2002 ◽  
Vol 162 (1) ◽  
pp. 273-284 ◽  
Author(s):  
Clark R Coffman ◽  
Rachel C Strohm ◽  
Fredrick D Oakley ◽  
Yukiko Yamada ◽  
Danielle Przychodzin ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Posterior Pole ◽  
Developmentally Regulated ◽  
Regulated Cell Death ◽  
Somatic Gonad ◽  
Programmed Death ◽  
Germ Cell Migration

Abstract Drosophila germ cells form at the posterior pole of the embryo and migrate to the somatic gonad. Approximately 50% of the germ cells that form reach their target. The errant cells within the embryo undergo developmentally regulated cell death. Prior studies have identified some autosomal genes that regulate germ cell migration, but the genes that control germ cell death are not known. To identify X-linked genes required for germ cell migration and/or death, we performed a screen for mutations that disrupt these processes. Here we report the identification of scattershot and outsiders, two genes that regulate the programmed death of germ cells. The scattershot gene is defined by a mutation that disrupts both germ cell migration and the death of germ cells ectopic to the gonad. Maternal and zygotic expression of scattershot is required, but the migration and cell death functions can be genetically uncoupled. Zygotic expression of wild-type scattershot rescues germ cell pathfinding, but does not restore the programmed death of errant cells. The outsiders gene is required zygotically. In outsiders mutant embryos, the appropriate number of germ cells is incorporated into the gonad, but germ cells ectopic to the gonad persist.

scholarly journals ABC-transporter activity and autocrine eicosanoid-signaling are required for germ cell migration a basal chordate

2018 ◽  
Author(s):  
Susannah H. Kassmer ◽  
Delany Rodriguez ◽  
Anthony DeTomaso
Keyword(s):  
Arachidonic Acid ◽  
Cell Migration ◽  
Germ Cell ◽  
Abc Transporter ◽  
Germ Cells ◽  
Signaling Molecules ◽  
Germline Stem Cells ◽  
Colonial Ascidian ◽  
Germ Cell Migration ◽  
12 Lipoxygenase

AbstractIn the colonial ascidian Botryllus schlosseri, long-lived germline stem cells (GSCs) migrate to new germline niches as they develop during repetitive cycles of asexual reproduction. ABC-transporters are involved in the export of lipid-signaling molecules, but their roles in germ cell migration are poorly understood. Here, we show that in Botryllus, abcc1 and abcb1 are highly expressed in germ cells, and inhibition of ABC-transporter activity leads to failure of germ cell migration. Phospholipase A2 (PLA2) produces arachidonic acid, which is further metabolized to eicosanoid signaling molecules. In humans, 12-lipoxygenase (LOX) metabolizes arachidonic acid to12-Hydroxyeicosatetraenoic acid (12-S-HETE), which stimulates migration of mammalian cancer cells and smooth muscle cells. We show that PLA2 and LOX activity are required for germ cell migration. A potential homolog to the human receptor for 12-S-HETE, BSgpr31, is expressed in germ cells. 12-S-HETE rescues migration towards S1P in the presence of inhibitors of ABCC1, ABCB1, PLA2 or LOX, and a gradient of 12-S-HETE enhances chemotaxis towards S1P and stimulates motility. We conclude that 12-S-HETE is a secondary chemoattractant exported by ACB-transporters that is required for migration of germ cells towards S1P. We also find that in the presence of S1P, detection of an 12-S-HETE gradient initiates an autologous positive feedback loop that may sustain migration. This is the first report of an eicosanoid-signaling molecule regulating germ cell migration.

Effects of 2,2',5,5'-tetrachlorobiphenyl (PCB52) on migration of chicken primordial germ cells

2005 ◽  
Vol 17 (5) ◽  
pp. 587 ◽  
Author(s):  
Yixiang Zhang ◽  
Xiumei Jin ◽  
Haitang Han ◽  
Zandong Li
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Direct Action ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Initial Stage ◽  
Germ Cell Migration ◽  
Cell Migration And Proliferation ◽  
Migration And Development

Polychlorinated biphenyls cause developmental and physiological anomalies in the reproductive system. This study investigated the effects of 2,2′,5,5′-tetrachlorobiphenyl (PCB52), which can produce oestrogenic effects on the homeostasis of chicken primordial germ cells from the initial stage until completion of their settlement in the gonadal primordium. The blastoderm of chicken embryos was injected with 1 μL PCB52 (10 µmol/L) and oestradiol (100 µmol/L) before incubation, and the number of primordial germ cells was determined during their migration and development. The number of primordial germ cells in germinal crescents in PCB52-treated groups was slightly decreased (P = 0.068), but it was reduced significantly at stages 13–15 and 28–30 (P < 0.01, respectively) compared with controls. No obvious effects on primordial germ cell migration were observed with oestradiol treatments. The present results suggest that the influence of PCB52 on chicken primordial germ cell migration and proliferation may be via its toxic effect, not its oestrogen-mimicking effect, and provide information on the sensitivity of primordial germ cells to the direct action of PCB52.

Mouse primordial germ cells lacking beta1 integrins enter the germline but fail to migrate normally to the gonads

Development ◽  
1999 ◽  
Vol 126 (8) ◽  
pp. 1655-1664 ◽  
Author(s):  
R. Anderson ◽  
R. Fassler ◽  
E. Georges-Labouesse ◽  
R.O. Hynes ◽  
B.L. Bader ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Migration ◽  
Germ Cell ◽  
Germ Cells ◽  
Fluorescent Protein ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Integrin Subunit ◽  
Integrin Beta1 ◽  
Germ Cell Migration

Primordial germ cells are the founder cells of the gametes. They are set aside at the initial stages of gastrulation in mammals, become embedded in the hind-gut endoderm, then actively migrate to the sites of gonad formation. The molecular basis of this migration is poorly understood. Here we sought to determine if members of the integrin family of cell surface receptors are required for primordial germ cell migration, as integrins have been implicated in the migration of several other motile cell types. We have established a line of mice which express green fluorescent protein in germline cells that has enabled us to efficiently purify primordial germ cells at different stages by flow cytometry. We have catalogued the spectrum of integrin subunit expression by primordial germ cells during and after migration, using flow cytometry, immunocytochemistry and RT-PCR. Through analysis of integrin beta1(−/−)--&gt;wild-type chimeras, we show that embryonic cells lacking beta1 integrins can enter the germline. However, integrin beta1(−/−) primordial germ cells do not colonize the gonad efficiently. Embryos with targeted deletion of integrin subunit alpha3, alpha6, or alphaV show no major defects in primordial germ cell migration. These results demonstrate a role for beta1-containing integrins in the development of the germline, although an equivalent role for * integrin subunit(s) has yet to be established.

scholarly journals Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration

Development ◽  
2016 ◽  
Vol 143 (12) ◽  
pp. 2111-2120 ◽  
Author(s):  
Girish Deshpande ◽  
Diane Manry ◽  
Nicholas Jourjine ◽  
Vladic Mogila ◽  
Henny Mozes ◽  
...  
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Abc Transporter ◽  
Hedgehog Signaling ◽  
Germ Cell Migration
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