The effect of 5-bromodeoxyuridine on cell division and differentiation of preimplantation mouse embryos
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Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from 0·01 to 1·0 μg/ml in vitro for two days from the 8-cell stage exhibit a concentration-dependent decrease in the frequency of normal blastocysts and a decrease in average cell number per embryo. A 20-h exposure was adequate to achieve the full BUdR response. Both effects were eliminated in the presence of excess thymidine. Autoradiographs demonstrated that BUdR[3H] was incorporated into DNA during the first and second day of culture. Thus, BUdR appears to act through incorporation into DNA; and, in this system, cell division is at least as sensitive to BUdR as is differentiation.
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1993 ◽
Vol 5
(3)
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pp. 271
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1972 ◽
Vol 30
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pp. 268-269
1988 ◽
Vol 76
(3)
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pp. 467-472
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