scholarly journals In vivo and in vitro studies on the early embryonic lethal oligosyndactylism (Os) in the mouse

Development ◽  
1979 ◽  
Vol 52 (1) ◽  
pp. 115-125
Author(s):  
Hugh F. Paterson
Keyword(s):  
Mutant Gene ◽  
Giant Cells ◽  
In Utero ◽  
Mouse Embryos ◽  
In Vitro Studies ◽  
Embryonic Lethal ◽  
Large Numbers

The development of mouse embryos homozygous for oligosyndactylism (Os) is arrested during implantation. Histological investigations confirm a previous report that cells become blocked in mitosis, and air-dried spreads of the mutant embryos reveal that large numbers of cells accumulate in metaphase. Trophoblastic giant cells appearunaffected by the action of the mutant gene both in utero and during culture over the lethal phase. It is proposed that the form of endoreduplication undergoneby giant cells renders them refractory to the metaphase block.

scholarly journals A critical role of VLA-4 in erythropoiesis in vivo

Blood ◽  
1996 ◽  
Vol 87 (6) ◽  
pp. 2513-2517 ◽  
Author(s):  
K Hamamura ◽  
H Matsuda ◽  
Y Takeuchi ◽  
S Habu ◽  
H Yagita ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fetal Liver ◽  
Critical Role ◽  
In Utero ◽  
In Vitro Studies ◽  
Specific Interactions ◽  
Erythroid Progenitors

Hematopoiesis requires specific interactions with the microenvironments, and VLA-4 has been implicated in these interactions based on in vitro studies. To study the role of VLA-4 in hematopoiesis in vivo, we performed in utero treatment of mice with an anti-VLA-4 monoclonal antibody. Although all hematopoietic cells in fetal liver expressed VLA-4, the treatment specifically induced anemia. It had no effect on the development of nonerythroid lineage cells, including lymphoids and myeloids. In the treated liver almost no erythroblast was detected, whereas the erythroid progenitors, which give rise to erythroid colonies in vitro, were present. These results indicate that VLA-4 plays a critical role in erythropoiesis, while it is not critical in lymphopoiesis in vivo.

Différentiation du trophoblaste au cours du développement in vitro d'embryon de Souris

Development ◽  
1971 ◽  
Vol 25 (2) ◽  
pp. 175-187
Author(s):  
Par D. Hernandez-Verdu ◽  
C. Legrand
Keyword(s):  
Cell Types ◽  
Giant Cells ◽  
In Utero ◽  
Trophoblast Cell ◽  
Mouse Embryos ◽  
Annulate Lamellae ◽  
Placental Barrier ◽  
In Vitro Development ◽  
Vitro Development

Differentiation of the trophoblast during in vitro development of the mouse embryo Mouse embryos obtained at the presomitic stage develop in vitro up to 70 h. During the first 24 h development remains comparable in vitro and in utero. Afterwards, the chronology of embryonic development is modified and in our successful prolonged experiments (70 h culture), foetal organogenesis reaches a stage corresponding only to 1½ days in utero. In some instances a chorioallantoic circulation has been observed in the umbilical cord. The three trophoblast cell types present in egg-cylinders at 8 days post coitum, develop in the same way in utero or in vitro. After 48 h culture electron microscopy shows that numerous annulate lamellae appear in the nuclei and in the cytoplasm of some of the giant cells. The syncytium does not differentiate between the trophoblast and the allantoic mesoderm. The haemo-trichorial placental barrier does not differentiate in vitro, although foetal capillaries form. In vitro, the endoderm cells (from the splanchnopleure or from the omphalopleure) actively proliferate and form an homogeneous substance located between the giant and the polyhedric trophoblast cells.

In vitro studies on the control of trophoblast outgrowth in the mouse

Development ◽  
1973 ◽  
Vol 30 (1) ◽  
pp. 21-30
Author(s):  
E. J. Jenkinson ◽  
I. B. Wilson
Keyword(s):  
Blastocyst Stage ◽  
Mouse Embryos ◽  
In Vitro Studies ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Uterine Environment ◽  
Adhesive Interactions

When placed in serum-free medium on reconstituted collagen surfaces re-implantation mouse embryos are capable of producing characteristic trophoblast outgrowths. Previously this pattern of differentiation has been considered to be essentially dependent on the presence of serum macromolecules. Such activity is expressed only at the late blastocyst stage and is qualitatively different from the adhesive interactions between blastomeres earlier in development. The development of the properties responsible for outgrowth is intrinsic to the blastocyst, being independent of stimulation by exposure either to the uterine environment or to whole serum. The significance of these observations related to implantation control in vivo is discussed.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

Ciamexon in low-dose streptozotocin induced diabetes mellitus. In vivo and in vitro studies

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S120-S121
Author(s):  
TH. LINN ◽  
H. GERMANN ◽  
B. HERING ◽  
R. BRETZEL ◽  
K. FEDERLIN
Keyword(s):  
Diabetes Mellitus ◽  
Low Dose ◽  
In Vitro Studies ◽  
Streptozotocin Induced Diabetes

Delayed hypersensitivity to tetanus toxoid in man: in vivo and in vitro studies

Pathology ◽  
1983 ◽  
Vol 15 (4) ◽  
pp. 369-372 ◽  
Author(s):  
Christine Johnson ◽  
R.S. Walls ◽  
A. Ruwoldt
Keyword(s):  
Tetanus Toxoid ◽  
In Vitro Studies ◽  
Delayed Hypersensitivity
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