The distribution of muscle fibre types in chick embryo wings transplanted to the pelvic region is normal

Development ◽  
1983 ◽  
Vol 78 (1) ◽  
pp. 67-82
Author(s):  
N. G. Laing ◽  
A. H. Lamb
Keyword(s):  
Chick Embryo ◽  
Muscle Fibre ◽  
Subsequent Development ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Pelvic Region ◽  
Lateral Motor Column ◽  
Acetylcholinesterase Staining ◽  
Slow Muscle Fibres ◽  
Atpase Staining

Chick embryo wing buds were transplanted to the pelvic region in place of, or in addition to, the hindlimb bud prior to innervation. The wrist muscle ulnimetacarpalis dorsalis (umd) was innervated by middle-dorsal or middle-ventral motoneurons in the lumbar lateral motor column (LMC) in a rostrocaudal position which varied with the rostrocaudal position of the wing. Despite the heterotopic innervation the subsequent development of the distributions of fast and slow muscle fibres, as judged by ATPase staining, was normal in all muscles examined. The pattern of innervation in the umd, as judged by acetylcholinesterase staining also developed normally. It is probable that muscle fibre type is intrinsically, not neurogenically, determined.

Muscle fibre types and innervation of the chick embryo limb following cervical spinal cord removal

Development ◽  
1985 ◽  
Vol 89 (1) ◽  
pp. 209-222
Author(s):  
N. G. Laing ◽  
A. H. Lamb
Keyword(s):  
Spinal Cord ◽  
Chick Embryo ◽  
Latissimus Dorsi ◽  
Cervical Spinal Cord ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Chick Embryos ◽  
Normal Numbers ◽  
Lateral Motor Column ◽  
Fibre Typing

Several segments of spinal cord were removed from the cervical regions of stage-13 or -14 day-2) chick embryos. After further incubation to day 17 or 18, the patterns of end-plate distribution and ATPase typing of muscle fibres in the anterior and posterior latissimus dorsi and the ulnimetacarpalis dorsalis, and the ATPase typing of the forearm muscles were examined. No differences from control embryos were found. The embryos had normal numbers of lateral motor column motoneurons in both the brachial and lumbar enlargements and the positions of motoneurons supplying the biceps as identified with retrograde horseradish peroxidase labelling were consistent with the normal patterns of motor projection into the limb. These results show that the fibre typing of limb muscles and their patterns of innervation are independent of descending inputs until just before hatching in the chick.

scholarly journals Development and motor innervation of a distal pair of fast and slow wing muscles in the chick embryo

Development ◽  
1983 ◽  
Vol 78 (1) ◽  
pp. 53-66
Author(s):  
N. G. Laing ◽  
A. H. Lamb
Keyword(s):  
Slow Muscle ◽  
The Other ◽  
Muscle Fibres ◽  
Motor Innervation ◽  
Lateral Motor Column ◽  
Spinal Segments ◽  
Slow Muscle Fibres ◽  
Acid Stable ◽  
Acid Labile ◽  
Atpase Staining

The chick wrist muscle ulnimetacarpalis dorsalis (umd) has two heads. Using myosin ATPase and acetylcholinesterase (ACh.E) staining it was shown that one of the heads is composed almost entirely of acid-stable muscle fibres with multiple end plates (slow muscle fibres) and the other of acid-labile fibres with single end plates (fast muscle fibres). The development of the muscle was traced from E7 (Stage 32–33) when it is a relatively homogeneous mass, to E18. The two heads of the muscle are first distinguishable, by ATPase staining, at E8 (Stage 33–34) prior to their cleaving. Both heads of the muscle are innervated by motoneurons positioned laterally in the lateral motor column in spinal segments 15 and 16. There is no observable difference in the positions of the motoneuron pools to the two heads. At E18 the motoneurons innervating the fast head tend to be slightly larger than those innervating the slow head.

Distributions of nerve and muscle fibre types in locust jumping muscle

1978 ◽  
Vol 73 (1) ◽  
pp. 205-233 ◽  
Author(s):  
G. Hoyle
Keyword(s):  
Muscle Fibre ◽  
Fibre Type ◽  
Muscle Fibre Type ◽  
Interference Microscopy ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Relaxation Rates ◽  
Mechanical Responses ◽  
Slow Type ◽  
Slow Muscle Fibres

Muscle fibres of the locust extensor tibiae (jumping muscle) were examined by interference microscopy and by electron microscopy. The electrical responses of single fibres and the mechanical responses of bundles or selected regions to the nerve fibres were examined. Four axons innervate the muscle: fast (FETi), slow (SETi), common inhibitor (CI) and dorsal unpaired median (DUMETi). Their distributions were examined by combined electrophysiological tracing and EM sectioning. The mean diameter of muscle fibres in different regions varies from 40 to 140 micrometer and is related to the local leg thickness rather than muscle fibre type. The fine structure of a fibre is related to its innervation. Fibres innervated by FETi but not SETi are of fast type ultrastructurally. Fibres innervated by SETi but not by FETi are of slow type ultrastructurally. Fibres innervated by both axons are generally intermediate between the extremes though more nearly of fast type than slow. Distal slow muscle fibres have much slower relaxation rates than do proximal ones. The most proximal bundles are of mixed muscle fibre type. There is an abrupt transition from a mixed population to homogeneous fast type, in the muscle units immediately distal to the most proximal bundles. This transition is associated with the presence of DUMETi terminals on some of the fibres distal to the transition point. There are no SETi endings on these same fibres. Fibres innervated by both SETi and FETi are scattered throughout the leg, but are commonest in the dorsal bundles. The percentage of these increases progressively passing distally. The most distal muscle fibres are innervated by SETi but not by FETi. It is concluded that different regions of the muscle will play different roles functionally since they are differentially sensitive to the pattern of SETi discharge.

Muscle growth in the Antarctic teleost, Notothenia neglecta (Nybelin)

1991 ◽  
Vol 3 (1) ◽  
pp. 29-33 ◽  
Author(s):  
John C. Battram ◽  
Ian A. Johnston
Keyword(s):  
Muscle Fibre ◽  
Muscle Growth ◽  
Somatic Growth ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Slow Muscle ◽  
Muscle Fibres ◽  
Slow Muscle Fibres ◽  
The Antarctic ◽  
Myotomal Muscle

A histochemical and electron microscopy study was carried out on muscle growth in demersal stages of the Antarctic teleost Notothenia neglecta Nybelin. The total number of myotomal muscle fibres was similar in fish ranging in body mass from 11.9g to 889g. Post-anal myotomes contained around 17,000 slow muscle fibres and 70,000 fast muscle fibres. Myosatellite cells were extremely rare. The diameter of fast fibres varied from <10μm to 130μm in the smallest, and from >40μm to 450μm in the largest fish studied. Slow muscle fibre diameters in the largest fish ranged from >30μm to 260μm. Even the largest diameter slow fibres contained significant numbers of mitochondria, which suggests that the diffusion of oxygen does not limit metabolism. The results confirm that muscle fibre hyperplasia ceases prior to the demersal stages of the life history, and that subsequent muscle growth is entirely via the hypertrophy of existing fibres. Comparative studies suggest that this may be one of the factors contributing to the relatively slow rate of somatic growth in this species.

scholarly journals Fibre types in the skeletal muscle of blackspot seabream (Pagellus bagaraveo, Brunnich) juveniles: a histochemical, immunohistochemical and ultrastructural study

2009 ◽  
Vol 15 (S3) ◽  
pp. 43-44
Author(s):  
P. Silva ◽  
L. M. P. Valente ◽  
F. Malhão ◽  
M. H. Galante ◽  
R. A. F. Monteiro ◽  
...  
Keyword(s):  
Muscle Fibre ◽  
Main Part ◽  
Histochemical Staining ◽  
Fibre Types ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Potential Candidate ◽  
Mitochondrial Content ◽  
Narrow Strip ◽  
Specific Antibodies

AbstractThe aim of this work was to gain insights into the muscle fibre phenotype and ultrastructure in blackspot seabream juveniles, a potential candidate for Portuguese aquaculture. Firstly, we used a panel of isoform-specific antibodies as well as histochemical staining for myosin ATPase and for SDH enzyme activity (a marker for mitochondrial content and oxidative metabolism) to identify myosin expression. Secondly, muscle fibre types were also identified by their fine structure where powerful approaches contributed to understand the muscle fibre phenotype of blackspot seabream juveniles. We observed that the main part of the musculature consisted of fast-white fibres, with a narrow strip of slow-red fibres situated superficially adjacent to the lateral line. A zone of intermediate-pink muscle fibres was inserted between those muscle domains (Fig.1).

Organization of skeletal muscle in the urodele Triturus cristatus : muscle fibre types and motor units

1985 ◽  
Vol 223 (1233) ◽  
pp. 495-510 ◽  
Keyword(s):  
Succinate Dehydrogenase ◽  
Motor Unit ◽  
Muscle Fibre ◽  
Lucifer Yellow ◽  
Motor Units ◽  
Fibre Types ◽  
Differential Staining ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Dehydrogenase Reaction

Four muscle fibre types are described in the biceps and extensor digitorum communis muscles of the newt forelimb. The histological criteria forming the basis for the distinctions include differential staining with p -phenylenediamine and succinate dehydrogenase histochemistry and electron microscopy. In addition, three distinctive motor unit types are described for the biceps muscle. These are fast units, slow units and intermediate units. The structure of muscle fibre and the physiological characteristics of muscle fibres belonging to each motor unit, have been correlated by using iontophoretic passage of Lucifer yellow into muscle fibres belonging to physiologically characterized motor units and their subsequent histological identification by the succinate dehydrogenase reaction. The three motor unit types correspond to slow muscle fibres, intermediate muscle fibres and two classes of fast muscle fibres.

scholarly journals The turnover of cytochrome c in different skeletal-muscle fibre types of the rat

1979 ◽  
Vol 178 (3) ◽  
pp. 569-574 ◽  
Author(s):  
R L Terjung
Keyword(s):  
Skeletal Muscle ◽  
Cytochrome C ◽  
Muscle Fibre ◽  
Skeletal Muscle Fibre ◽  
Fibre Types ◽  
Male Rats ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Double Labelling ◽  
Fast Twitch

The turnover of cytochrome c was determined in the three skeletal-muscle fibre types of adult male rats by a kinetic analysis that followed the time course of cytochrome c content change. Confirming evidence was obtained with double-labelling studies using delta-aminolaevulinate. Cytochrome c turnover was most rapid in the low-oxidative fast-twitch white fibre [t1/2 (half-life) about 4 days], slowest in the high-oxidative fast-twitch red fibre (t1/2 9-10 days) and relatively rapid in the high-oxidative slow-twitch red fibre (t1/2 5-6 days). Thus cytochrome c turnover does not strictly conform to either the appearance (i.e. red or white) or the contractile characteristics (i.e. fast or slow) of the muscle fibres. The synthesis rates needed to maintain the corresponding cytochrome c concentrations, however, were similarly high in the two mitochondria-rich red fibre types. These data illustrate that both the synthesis and degradation processes are important in establishing the cytochrome c concentrations that distinguish the different skeletal-muscle fibre types.

Tacrolimus enhances the recovery of normal laryngeal muscle fibre distribution after reinnervation

2012 ◽  
Vol 126 (11) ◽  
pp. 1155-1158 ◽  
Author(s):  
P Gorphe ◽  
N Guerout ◽  
M Birchall ◽  
G Terenghi ◽  
J-P Marie
Keyword(s):  
Muscle Fibre ◽  
Low Dose ◽  
Immunohistochemical Analysis ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Healthy Group ◽  
Laryngeal Muscle ◽  
Laryngeal Reinnervation ◽  
Posterior Cricoarytenoid ◽  
Resection And Anastomosis

AbstractObjectives:To assess the recovery of various muscle fibre types in the posterior cricoarytenoid muscle after laryngeal reinnervation in the rat, and to determine the influence of tacrolimus on this process.Methods:Four groups of rats underwent resection and anastomosis of the left vagus nerve, and were administered either tacrolimus at a low dose or an immunosuppressive dose, or cyclosporin A at a low dose or an immunosuppressive dose. A fifth group received surgery alone, and a sixth group received neither surgery nor drug treatment (healthy group). Muscles were removed for immunohistochemical analysis 45 days after surgery.Results:There was no difference in the proportion of types 1, 2a and 2b muscle fibres, comparing the immunosuppressive tacrolimus group and the healthy group, whereas there were fewer type 1 fibres in the group receiving surgery alone, compared with the healthy group (7 vs 12.1 per cent, respectively; p = 0.0303).Conclusion:Tacrolimus enhanced the recovery of normal laryngeal muscle fibres after reinnervation in the rat, indicating a possible role in laryngeal transplantation.

scholarly journals Composition and cross-sectional area of muscle fibre types in relation to daily gain and lean and fat content of carcass in Landrace and Yorkshire pigs

1996 ◽  
Vol 5 (6) ◽  
pp. 593-600 ◽  
Author(s):  
Marita Ruusunen ◽  
Marja-Liisa Sevon-Aimonen ◽  
Eero Puolanne
Keyword(s):  
Muscle Fibre ◽  
Live Weight ◽  
Carcass Composition ◽  
Cross Sectional Area ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Type I ◽  
Sectional Area ◽  
Cross Sectional ◽  
Yorkshire Pigs

The muscle fibre-type properties of longissimus were compared between Landrace and Yorkshire breeds and between the sexes in an attempt to shed light on the relationship of these histochemical parameters to animal growth and carcass composition. Muscle fibres were classified into three groups, type I, type lIA and type 11B, using the myosin ATPase method. At a given live weight, the cross-sectional area of type I fibres (CSA1) was smaller (p

scholarly journals Preferred metabolic pathway of bovine muscle fibre revealed by synchrotron–deep ultraviolet fluorescence imaging

2019 ◽  
Author(s):  
Thierry Astruc ◽  
Olivier Loison ◽  
Frédéric Jamme ◽  
Matthieu Réfrégiers ◽  
Annie Vénien
Keyword(s):  
Fluorescence Imaging ◽  
Muscle Fibre ◽  
Cross Sections ◽  
Metabolic Pathway ◽  
Fibre Types ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Label Free ◽  
Bovine Muscle ◽  
Deep Ultraviolet

The different bovine muscle fibre types I, IIA and IIX are characterised by their preferred metabolic pathway, either oxidative (I, IIA) or glycolytic (IIX), and their contraction speed, either slow-twitch (I) or fast-twitch (IIA, IIX). These physiological specificities are associated with variations in intracellular composition and their fluorescence spectra signatures. We hypothesised that these slight differences in autofluorescence responses could be used to discriminate the muscle fibre types by fluorescence imaging. Serial histological cross-sections of beef longissimus dorsi were performed: the start set was used to identify the metabolic and contractile type of muscle fibres by both immunohistoenzymology and immunohistofluorescence, and the following set was used to acquire synchrotron–deep ultraviolet (UV) autofluorescence images after excitation in the UV range (275 nm and 315 nm). This strategy made it possible to explore the label-free autofluorescence of muscle cells previously subtyped by histochemistry. Glycolytic cells (IIX) showed more intense fluorescence than oxidative cells (I and IIA) with near-90 % accuracy. This discrimination is more specifically assigned to the fluorescence of nicotinamide adenine dinucleotide. UV autofluorescence was unable to discriminate contractile type.

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