scholarly journals A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

2002 ◽  
Vol 115 (21) ◽  
pp. 4191-4200 ◽  
Author(s):  
Y. Saito
Keyword(s):  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Periodontal Ligament ◽  
Bone Morphogenetic Protein 2 ◽  
Periodontal Ligament Fibroblasts ◽  
Morphogenetic Protein ◽  
A Cell

Differentiation of human pluripotent teratocarcinoma stem cells induced by bone morphogenetic protein-2

1998 ◽  
Vol 10 (8) ◽  
pp. 551 ◽  
Author(s):  
Martin F. Pera ◽  
Daniella Herszfeld
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Retinoic Acid ◽  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Morphological Changes ◽  
Bone Morphogenetic Protein 2 ◽  
Stem Cell Markers ◽  
Potent Inducer ◽  
Morphogenetic Protein

Pluripotent human teratocarcinoma stem cells cultured in vitro provide a resource for the study of early embryonic development in man, as well as a means for discovery of novel factors controlling cell differentiation and commitment. We previously reported that the human teratocarcinoma stem cell line GCT 27X-1 could be induced to differentiate into an endodermal progenitor cell by treatment with high doses of retinoic acid. A search for polypeptide inducers of differentiation in this system has identified bone morphogenetic protein-2 (BMP-2) as a potent inducer of differentiation. In cell line GCT 27X-1, treatment with BMP-2 reduces proliferation, induces morphological changes similar to obtained following treatment with retinoic acid, and causes a decrease in the expression of transcripts for the stem cell markers CD30 and Oct-4. Preliminary immunochemical studies indicate that the differentiated cells produced by BMP-2 are endodermal precursors with a pattern of marker expression similar to that found in retinoic acid treated cells. Models of endoderm differentiation in humans will be useful for identifying the molecules which mediate cell interactions in development, and in achieving directed differentiation of cells for use in transplantation.

Establishment of bone morphogenetic protein 2 responsive chondrogenic cell line

2009 ◽  
Vol 11 (4) ◽  
pp. 544-553 ◽  
Author(s):  
Tomonao Aikawa ◽  
Kanemitsu Shirasuna ◽  
Masahiro Iwamoto ◽  
Kazuya Watatani ◽  
Takashi Nakamura ◽  
...  
Keyword(s):  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein
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