Circus movement in dissociated embryonic cells of a teleost, Oryzias latipes

1975 ◽  
Vol 19 (1) ◽  
pp. 169-182
Author(s):  
N. Fujinami ◽  
T. Kageyama
Keyword(s):  
Oryzias Latipes ◽  
Time Lapse ◽  
Cell Diameter ◽  
Fresh Water Fish ◽  
C Cells ◽  
Embryonic Cells ◽  
Salt Solutions ◽  
Circus Movement ◽  
Water Fish ◽  
Effects Of Ph

The dissociated early embryonic cells of the fresh water fish, Oryzias latipes, protrude hyaline lobopodia, which tend to rotate around the cell circumference in a propagating wave. Cells from late blastula or gastrula continuously show this “circus movement”, while most cells up to early blastula are rounded. The linear velocity of the lobopodium was estimated by means of time-lapse cinemicrography. The velocity increases slightly as cell diameter increases. The effects of pH, temperature and osmotic pressure of the immersion media on the movement were also quantitatively investigated. Cells become rounded and do not form lobopodial blebs when immersed in media below pH 5. The velocity is reduced by decreasing temperature, but the movement continues even at 5 degrees C. Cells placed in hypertonic salt solutions become crenated and do not continuously demonstrate the circus movement.

Studies on the mechanism of circus movement in dissociated embryonic cells of a teleost, Oryzias latipes: fine-structural observations

1976 ◽  
Vol 22 (1) ◽  
pp. 133-147
Author(s):  
N. Fujinami
Keyword(s):  
Cytochalasin B ◽  
Oryzias Latipes ◽  
Cortical Layer ◽  
Dense Material ◽  
Dense Layer ◽  
Cell Periphery ◽  
Embryonic Cells ◽  
Advancing Front ◽  
Circus Movement

The fine structure of lobopodia in dissociated embryonic cells of the freshwater fish, Oryzias latipes, was observed with the electron microscope in order to understand the mechanism of the circus movements which they display. Dense material (grandular or fibrillar) is present in the zone between the lobopodium and the endoplasm, as well as in the cortical layer around the cell circumference. The direction of lobopodial movement is related to the distribution of this dense material. The band between the lobopodium and the endoplasm is conspicuous and is connected to the cortical dense layer around the cell periphery at the advancing front of the lobopodium, while the dense material is usually almost absent beneath the cell membrane in the anterior region of the lobopodium. The band between lobopodium and endoplasm is blurred or disrupted near the hind end of the lobopodiu, where the peripheral dense layer is well developed. In situ localization of actin/heavy meromyosin complexes in the cell showed that the dense material has actin-like properties. Cytochalasin B(0-5 mug/ml) induced constriction of the neck of the bleb, shrinkage of the endoplasm, and herniation of the endoplasmic contents to the enlarged hemispherical bleb, and thus arrested the circus movement. On the basis of these results, an hypothesis concerning the mechanism of circus movement is proposed and discussed.

scholarly journals Fresh-water Fish Chemoreceptors Responsive to Dilute Solutions of Electrolytes

1966 ◽  
Vol 49 (6) ◽  
pp. 1241-1264 ◽  
Author(s):  
JIHEI KONISHI
Keyword(s):  
Supporting Electrolyte ◽  
Fresh Water Fish ◽  
Distilled Water ◽  
Monovalent Cations ◽  
Water Effect ◽  
Salt Solutions ◽  
Dilute Solutions ◽  
Water Fish ◽  
Solutions Of Electrolytes ◽  
Positively Charged

The existence of the palatal chemoreceptors responding specifically to dilute solutions of salts with monovalent cations was demonstrated in carp. The distilled water effect (a response produced by the application of distilled water after chemoreceptors had been rinsed out with hypertonic salt solutions) was assigned to the activity of the same receptor. Intensity of the response to dilute solutions of salts depended on the valency of the anion: the larger the valency, the greater the response. Positively charged sites of the receptor responsive to dilute salt solutions were suggested by previous treatments with acid and alkali, and dye salts. Increase in the ionic strength of the stimulating solution by the addition of supporting electrolytes caused a depression of response. In particular, strong depression of response was caused by the addition of a supporting electrolyte with a divalent cation. Effects of polarizing current on the chemoreceptor activity were investigated. Based upon the findings in this paper, a hypothesis is presented, which explains mechanisms underlying chemoreceptor responses to dilute solutions of electrolytes in terms of an interfacial electro-kinetic process.

DESCRIPTION OF CIRCUMONCOBOTHRIUM SINGHII N.SP. FROM CLARIAS BATRACUS OF SAVITRI RIVER AT DAPOLI DISTRICT RATNAGIRI (M.S.) INDIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
R.R. DANDAWATE
Keyword(s):  
Life Cycle ◽  
Fresh Water ◽  
Summer Season ◽  
Fresh Water Fish ◽  
Water Fish

Present paper deals with study of cestode parasites of genus Circumoncobothrium from fresh water fish Clarius batracus at Savitri river, (Dapoli) for the percentages of infection occurance during summer season,minimum during winter and tolarate during rainy season.The parasite mainly infected the intestine of host and fed on nutrients from digested food. It completed its life cycle in the intestine of host. By camparing different characters of it to identify that the species is new

PREVALENCE OF PSEUDOINVERTA ORAIENSIS IN FRESH WATER FISH, CLARIAS BATRACHUS

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
ADITYA NARAYAN
Keyword(s):  
Fresh Water ◽  
Monsoon Season ◽  
Winter Season ◽  
Uttar Pradesh ◽  
Summer Season ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Water Fish

The present investigation deals with the prevalence of infection of cestode, Pseudoinverta oraiensis19 parasitizing Clarias batrachus from Bundelkhand Region (U.P.) India. The studies were recorded from different sampling stations of Bundelkhand region of Uttar Pradesh. For this study 360 fresh water fish, Clarias batrachus were examined. The incidence of infection, monsoon season (17.50%) followed by winter season (20.00%) whereas high in summer season (30.00%).

Impact of Chromium on Lactate in different types of muscles of a fresh water fish, Labeorohita

2012 ◽  
Vol 3 (6) ◽  
pp. 483-484
Author(s):  
M. Cynthia Sailaja ◽  
◽  
G. Vijay Kumar ◽  
K. Jayantha Rao
Keyword(s):  
Fresh Water ◽  
Fresh Water Fish ◽  
Water Fish ◽  
Different Types

Toxic effects of sub lethal concentration of dioctyl phthalate on the histology of liver of Clarias batrachus (Linn.).

2016 ◽  
Vol 5 (09) ◽  
pp. 4874
Author(s):  
Manisha Satpathi* ◽  
Ravinder Singh
Keyword(s):  
Acute Toxicity ◽  
Light Microscopy ◽  
Dioctyl Phthalate ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Water Fish ◽  
Toxicity Effects ◽  
Plastic Products

Dioctyl phthalate (DOP) commonly used as plasticizer enters into aquatic environment from the industries manufacturing plastic products, PVC resins, cosmetics and many other commercial products. Therefore, this study was designed to evaluate the acute toxicity effects of Dioctyl phthalate on fresh water fish Clarias batrachus. The 96 hour LC50 of Dioctyl phthalate in Clarias batrachus was estimated as 14.5ml/L. Histopathological changes in liver of Clarias batrachus were determined by exposing them to a fraction of LC50(1/5th) dose with every change of water for 30 days.The liver of Clarias batrachus was excised at every interval of 30 days and histological changes of liver were studied under light microscopy. Liver tissues showed abnormalities. Centrilobular vacuolation, necrosis, eccentric nuclei and enlarged nuclei, Centrilobular degeneration of hepatocytes were observed in liver tissue of fish.

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